Seed germination of five Helianthemum species: Effect of temperature and presowing treatments

The germination behaviour of five Helianthemum species (H. almeriense, H. appeninum, H. cinereum, H. hirtum, H. squamatum) has been studied under controlled conditions. Constant 15, 20 or 25 °C and alternating 25/15 °C temperature regimes and 16/8 h light/dark photoperiod conditions were used. Presowing treatments applied were manual scarification, boiling water, hot water, dry heat and sulphuric acid. Germination values recorded were final germination percentage and germination rate expressed as days to reach 50% of the final germination percentage (T₅₀). Incubation temperature had no significant effect on final germination percentage for untreated seeds of the five Helianthemum species. However, variation due to temperature was significant for scarified seeds, with the lowest germination percentage attained at 25 °C. In all Helianthemum species studied, the highest germination percentages were obtained by manual scarification of seeds. Germination rate of scarified seeds decreased as germination temperature increased. The different presowing treatments investigated allowed some germination in some species, but none were any better than manual scarification. The high germination among most species studied, following mechanical rupture of the seed coat, shows that the mechanism of dormancy lies in the seed coat. The physical dormancy caused by impermeable seed-coat appears to be the main reason of poor germination of untreated seeds of Helianthemum species studied.     

Plant life-form and germination in a Mexican inter-tropical desert: effects of soil water potential and temperature

We investigated the effects of soil water potential (SWP) and temperature on seed germination of six coexisting species of an inter-tropical desert. These species include three life-forms; the shrubs Cercidium praecox andProsopis laevigata ; the columnar succulents Neobuxbaumia tetetzo and Pachycereus hollianus; and the arborescent semi-succulents Beaucarnea gracilis and Yucca periculosa. In the six species germinability increased and germination time and speed of germination (t⁵⁰) decreased as SWP decreased. The SWP treatments were 0 MPa, −0·12 MPa, −0·2 MPa, −0·41 MPa and −0·66 MPa. There was, however, a SWP threshold below which germination time and t⁵⁰increased and germinability decreased. The shrubs had the highest germinability whereas the columnar succulents had the lowest. The shrubs also had shorter germination time andt⁵⁰ than arborescent semi-succulents whereas seeds of the columnar succulents were indeterminate. In all species except P. laevigata germinability increased and the germination time and t⁵⁰decreased as temperature increased. The temperature treatments were 12°C, 20°C and 26°C. The shrubs had the shortest t⁵⁰and germination time and the highest germinability at all temperatures. Arborescent semi-succulents had the lowest germinability and longest germination time and t⁵⁰at the three temperatures treatments. Our results support the hypothesis that in desert environments different plant life-forms utilise different germination strategies to persist.     

Seed germination of five mountain steppe species of Central Asia

This paper presents data on the germination traits of five perennials (Allium polyrrhizum, Agropyron cristatum, Arenaria meyeri, Artemisia frigida and Artemisia santolinifolia) widespread in the mountain steppes of southern Mongolia. Germination and seed viability were assessed at three alternate temperatures (8/4 °C, 20/10 °C, 32/20 °C), three levels of osmotic stress (deionized water; −0.5 MPa, −1 MPa Mannitol solution), and under conditions of alternate light/darkness versus complete darkness. The results of a factorial ANOVA with treatments and species as main effects showed that all five species germinated best at higher temperatures, with only Agropyron cristatum showing some seed mortality. Osmotic stress reduced seed viability and total germination in all five species. Darkness had no influence on viability, but positively affected seed germination of Allium polyrrhizum and Agropyron cristatum. We therefore conclude that, in the field, germination of all five species is mainly controlled by ambient temperatures and water availability, both of which drop towards the end of summer when dispersal takes place and effectively delay seedling recruitment until the next vegetation period.     

Phenology, and seed production and germination of seven endemic Mimosa species (Fabaceae-Mimosoideae) of the Tehuacán-Cuicatlán Valley, Mexico

We report on the phenology, seed production, and three assays (field, greenhouse and laboratory) to detect the optimal temperature for germination (OPT) and the temperature with fastest germination rate (TFASG) of seven Mimosa species, all endemic to Mexico: Mimosa adenantheroides (M. Martens and Galeotti) Benth., M. calcicola B. L. Rob., M. lacerata Rose, M. luisana Brandegee, M polyantha Benth., M. purpusii Brandegee and M. texana (A. Gray) Small var. filipes (Britton and Rose) Barneby. Flowering and fruiting take place during the wet season (May–September). Though flowering periods overlap among species, no hybrid species were found. The reproductive strategy includes a large number of seeds produced (4000–15000 per plant); however, a high percentage (30–75%) is not viable due to bruchid predation (Acanthoscelides mexicanus (Sharp) and Stator pruininus Horn). In the laboratory, OPT was established at (23°C)24°C(27–28°C) and TFASG at (20°C)25°C(30°C), with 100% of germination in six of the seven Mimosa species. In the field, the maximum germination rate (MAXG) was 92% to 100%, whereas in the greenhouse this value was 56% to 80%. Our results show that heat and moisture do not necessarily stress Mimosa seedling germination, but shade seems to be an important factor influencing germination.     

Germination of Callitris seeds in relation to temperature, water stress, priming, and hydration–dehydration cycles

The effects of temperature, water stress, hydration–dehydration cycles and seed priming on the germination of Callitris verrucosa and Callitris preissii, two Australian semi-arid coniferous tree species, were investigated. Optimum germination occurred at 18°C, with a minimum germination time of 8–9 days for both species. At this temperature, germination was inhibited at osmotic potentials lower than −1·0 MPa, but the capacity to germinate at low osmotic stress increased as the temperature decreased. Seed priming and hydration–dehydration cycles did not reduce seed viability, and Callitris seeds appear to retain the physiological changes induced by short-term hydration, as the time to the onset of germination was decreased to about 3 days. The capacity of Callitris seeds for incremental germination is likely to increase overall germination success in a low rainfall environment.     

Effects of salinity, temperature and light on germination of invasive Prosopis juliflora (Sw.) D.C.

Effects of salinity, temperature, light and their interactions on the rate and final percentage of germination were evaluated for the invasive shrub Prosopis juliflora, grown under arid environmental conditions of the United Arab Emirates (UAE). Seeds that were not treated with NaCl germinated well in a wide range of temperatures and in both full light and complete dark. Seed germination decreased significantly with the increase in both NaCl concentration and temperature. Optimal germination percentage occurred at 25 °C. The inhibitory effect of high salinity on final germination percentage was greater at 40 °C than at 15 and 25 °C. Germination was completely inhibited in 400 mM NaCl at 40 °C and in 600 mM NaCl at 25 °C. However, germination rate was significantly greater at 40 °C than at 15 and 25 °C in lower salinities and the reverse was true at higher salinities. Germination in light was significantly greater than in the dark at lower salinity levels and high temperature.     

Effects of body mass and temperature on standard metabolic rate of the herbivorous desert lizardUromastyx philbyi

The standard metabolic rate ofUromastyx philbyiwas measured at 20–40 °C, using constant pressure manometric respirometers. Standard metabolic rate was mass-dependent. Values for the mass exponent ‘b’ ranged from 0·75 at 20 °C to 0·82 at 40 °C. Standard metabolic rate increased as temperature increased with high Q₁₀values at low temperatures (20–25 °C). Low standard metabolic rates in this species are probably an adaptation to herbivory in hot desert environments.     

Effects of indole-3-acetic acid and zinc on the growth, osmotic potential and soluble carbon and nitrogen components of soybean plants growing under water deficit

Three-week old soybean (Glycine max) plants were subjected to a factorial combination of four regimes of soil matric water potential (ψ_m=−0·03, −0·5, −1·0 and −1·5 MPa), two levels of supplementary Zn (O and 20 mgl⁻¹) and two levels of foliar IAA application (O and 10 mgl⁻¹). Under control conditions (no Zn, no IAA), increasing soil drying progressively retarded shoot and root growth (length and dry mass production), reduced leaf relative water content (RWC) and decreased the contents of chlorophyll (Chl) and shoot soluble sugars (SS), but increased soluble sugar content of roots and lowered osmotic water potential of shoots and roots (osmotic adjustment). Total free amino acid (TAA) content increased in shoots but decreased in roots whereas contents of soluble proteins (SP) decreased in shoots and roots. The effect of water stress was statistically significant (p<0·05) and had a major effect (as indicated by η²values) on leaf RWC, shoot and root dry masses and osmotic potential. Supplementary Zn improved root growth at all levels of stress and shoot growth under severe stress. Improvement of growth was positively correlated with the internal tissue Zn concentrations (r=0·91 and 0·86 for shoot and 0·94 and 0·82 for root length and dry mass respectively). Exogenous IAA raised (p<0·05) RWC, Chl, DM (slightly), root SS, and SP, whereas shoot TAA was lowered. Effects on root TAA and shoot SS were more complex: they were lowered at zero stress and raised under severe stress. IAA and Zn in combination had additive effects on Chl, growth and osmotic potential, but their combined effects on SP and TAA were more complex. It is concluded that the treatment of soybean plants grown under conditions of low soil water potentials and Zn deficiency with Zn and IAA solutions counteracted the deleterious effects of stress, especially at high stress levels, and helped stressed plants to grow successfully under these adverse unfavourable conditions.     

Water requirements for germination and early seedling establishment in four African savanna woody plant species

The aim of this study was to determine the water requirements for germination and early seedling establishment of four African savanna tree species, namelyAcacia karroo, A. nilotica, A. tortilisandMundulea sericea. The acacias are characteristic of nutrient-rich, andM. sericeaof nutrient-poor savannas. Imbibition times of scarified seeds ofA. karroo(4 h),M. sericea(6 h) andA. tortilis(8 h) were rapid relative toA. nilotica(28 h) and were inversely correlated with seed size. Imbibed water is lost in about 2 h after drying at 25°C for all species exceptA. nilotica(only 70% moisture loss). Seeds ofA. karrooandM. sericeakept at 40°C dried to below their original moisture contents within 2 h. Seeds ofA. niloticaandA. tortilisdried at 40°C lost viability relative to (undried) control seeds, while there was no significant loss of viability forA. karrooandM. sericea. For fully imbibed seeds to germinate in sandy savanna soils, all four species required at least the equivalent of 3 mm rainfall every 2 days under mild greenhouse conditions. However, most ungerminated seeds were still viable despite drying-out from a fully imbibed state. Maintenance of soil at 50% field capacity (FC), or watering to FC every 9th day is the maintenance requirements for 2-week-old seedlings ofA. niloticaandM. sericeato continue growth for a further 5 weeks under greenhouse conditions, althoughA. niloticamostly survived 25% FC with one seedling surviving 12·5% FC. Root penetration was rapid in sandy soils, withA. niloticaandM. sericeaattaining a depth of 40 cm within 15 days. Frequent, but not necessarily high, rainfall appears to be essential for germination and seedling survival over the first 7 weeks.     

Effect of light on germination of seven species of cacti from the Zapotitlán Valley in Puebla, México

Seed germination of seven species of cacti from Zapotitlán Valley, Puebla, Mexico, were compared at four different light treatments (red light, far-red light, white light and darkness) at a constant temperature of 25°C, and at two light treatments (white light and darkness) at two fluctuating temperatures (15–30°C and 20–30°C). Results allowed us to divide the studied species into two groups: positive photoblastic and indifferent to light. Positive photoblastics were barrel cacti and indifferent to light species were columnar cacti. Although barrel cacti had a light requirement for germination, they germinated in far-red light. Probable relationships among life-form, species distribution and light requirements for germination are discussed.     

Soil seed flora, germination and regeneration pattern of woody species in an Acacia woodland of the Rift Valley in Ethiopia

The objectives of this study were to determine the composition, density and spatial distribution of the soil seed bank of woody species, as well as their regeneration pattern in two different land use systems, controlled (ranch) and open grazing, in an Acacia woodland of the Rift Valley in Ethiopia. We also compared the species composition of the soil seed bank and the above-ground vegetation to find out if differences exist in the soil seed bank and advance regeneration between the two land use systems. The germination requirements of seeds of the woody species were also investigated under laboratory conditions. Acacia senegal, Acacia seyal, Acacia tortilis, Dichrostacys cinerea and Balanites aegyptiaca were encountered in the above-ground vegetation in both systems. Seeds of only A. tortilis (90±32 seeds m⁻²) from the ranch, and A. senegal (5±3 seeds m⁻²) and A. tortilis (72±34 seeds m⁻²) from the open system were found in the soil seed bank along transects with patchy horizontal pattern. The two systems were not significantly different in density of soil seed banks of A. senegal andA. tortilis . Jaccard's similarity index showed that only a few woody species were common in the soil seed flora and above ground vegetation. However, all of the species accumulated seeds (58±43–331±130 seeds m⁻²) in/on the soil under the canopy. Very large numbers of seeds of A. tortilis (19382±9722 seeds m⁻²) and D. cinerea (1278±494 seeds m⁻²) were also found in barns. Most of the seeds recovered from the soil samples (60–80%) were found in the litter layer. Acid and mechanical scarification improved legume germination (36–99% and 60–99%, respectively) over boiling water (0–48%). Treatment means differed significantly for all the legumes (p<0·001) but not for Balanites. Height and diameter class distribution of regeneration of A. tortilis and D. cinerea in both systems and A. senegal in the open system had a negative exponential correlation (r_s=−0·5, −0·25 and −0·86, respectively). A. seyal and B. aegyptiaca showed poor regeneration. Horizontal distribution of advance regeneration of all the species was patchy. Advance regeneration of A. seyal, A. tortilis and B. aegyptiaca were not significantly different, while that of A. senegal and D. cinerea were significantly different between the two systems. Poor representation of species in the soil seed bank along transects and in the different height and diameter classes may be attributed to the low density of mature trees as well as the mode and strategy of seed dispersal. Ungulate and wind dispersed species (e.g. A. senegal, A. tortilis and D. cinerea) were highly favoured. Patchiness in the distribution of seeds and advance regeneration was also a result of endozoochory. Dispersal of non-ungulate dispersed seeds (A. senegal, A. seyal and B. aegyptiaca) was restricted to the canopy zone. Piles of seeds ofA. tortilis and D. cinerea that were found in barns were a result of consumption of their pods by cattle. High concentration of seeds in the litter layer may be due to low soil disturbance and larger size of seeds. The height and diameter class distribution of A. senegal (in the open system), A. tortilis and D. cinerea also indicated that the species have good regeneration. Results from the germination tests indicated that seeds of the legumes require pre-sowing treatments to give a rapid, uniform and improved germination. Intervention through artificial regeneration should be employed to improve the density and regeneration capacity of those species with hampered regeneration at both systems.     

Critical conditions for successful regeneration of an endangered annual plant,Cryptantha minima: A modeling approach

Tiny cryptanthe (Cryptantha minima Rydb.) is an endangered annual plant adapted to prairie sand dunes of Canada. Thermal time and hydrothermal time models were used to quantify the effect of temperature and water potential on seed germination and to relate it to field conditions. Tiny cryptanthe seeds had a base temperature of ?3.9 °C for germination. Final germination percentage was highest at temperatures near the freezing point. Seeds required approximately 130.6 °C d to reach 50% germination under optimal water conditions. Seed germination was sensitive to water stress and large seeds had lower germination than small ones especially at reduced water potentials and higher temperatures. The high potential germination and low seed dormancy indicate that tiny cryptanthe lacks a dormancy mechanism, but the unique germination response to temperature enables it to fully utilize water from spring snowmelt before drought in the summer. Future climate change with higher temperatures could reduce regeneration success of this species unless there is an associated increase in winter and spring precipitation.     

Microclimate, freezing tolerance, and cold acclimation along an elevation gradient for seedlings of the Great Basin Desert shrub, Artemisia tridentata

Vegetation, microclimate, seedling frequency, freezing tolerance, and cold acclimation were compared for seedlings of Artemisia tridentata collected from 1775, 2175, and 2575 m elevation in the eastern Sierra Nevada, California. Data were used to test the hypothesis that ecotypic differences in stress physiology are important for seedling survival along gradients from desert to montane ecosystems. The vegetation canopy cover and A. tridentata seedling frequency were greatest at 2575 m, compared to 1775 and 2175 m. Snow cover ameliorated temperatures near the soil surface for part of the winter and depth varied across elevations. Freezing tolerance was compared for seedlings maintained in growth chambers at day/night air temperatures of 25°C/15°C. The temperature at which electrolyte leakage and Photosystem II function (F_V/F_M) from leaves were half-maximum was approximately −13·5°C for leaves of seedlings from all three elevations. Shifting day/night air temperatures from 25°C/15°C to 15°C/5°C initiated about 1·5° of acclimation by plants from all three altitudes, with seedlings from the highest elevation exhibiting the greatest acclimation change. Measurements of ambient air and canopy temperatures at the three elevations indicated that wintertime average low temperatures were consistent with the measured degree of freezing tolerance. At small spatial scales used in this study, pollen and seed dispersal between study sites may have precluded resolution of ecotypic differences. Patterns of freezing tolerance and cold acclimation may depend on a combination of mesoclimate and microclimate temperatures, canopy cover, snow depth, and snow melt patterns.     

Selective grazing by cattle on spatially and seasonally heterogeneous rangeland in Sahel

The selective use by cattle of range resources in the Sahel was assessed in terms of quantity and nutritional quality of the herbage grazed and diet selected. Peak standing crop was 1951 kg ha⁻¹dry matter (DM) with 47·9 kg nitrogen (N) ha⁻¹and 7·4 kg phosphorus (P) ha⁻¹. Esophageally fistulated cattle diets were consistently (p<0·05) higher in N, P and organic matter digestibility (OMD) than the herbage grazed. Cattle consumed 48% of the mean annual herbage production. The results indicate grazing ruminants select a higher quality diet from Sahelian rangelands than can be predicted on the basis of pasture evaluation alone. Therefore, the foraging behaviour of the animals needs to be considered in the evaluation of Sahelian rangelands for animal production.     

Biodegradation of municipal refuse and chicken manure in a winter-wheat ecosystem in Saudi Arabia

Addition and incorporation of organic fertilizers is known to improve the properties of agricultural soils. Therefore, during 22 weeks of field incubation we studied the biodegradation (measured by static absorption of CO₂) of two organic manures viz. municipal refuse and chicken manure incorporated into sandy soils, under sprinker irrigation, at the rates of 0, 16·5, 33·0, 49·5 and 66·0, and 0, 4·1, 8·25, 16·5, and 33·0 tonnes/ha, respectively.The results obtained indicated that the cumulative amount of CO₂ respired was linearly correlated with the rate of organic material applied (p < 0.001). Statistical analysis of the data obtained showed that both the intercept (rate of decomposition) and the slope (rate of reaction with time) increased with increasing rate of application of organic manure. The magnitude of increment was highest at the application rates of 33·0 and 8·25 tonnes/ha for municipal refuse and chicken manure, respectively. The rate of increment, generally, declined thereafter thus indicating that these two rates are the optimum levels of incorporation of the appropriate organic manures under the conditions of this study.The maximum organic-carbon mineralized, accounting for 10·5 and approximately 50%, was obtained at the optimum rates for municipal refuse and chicken manure, respectively. Both simple and multiple regression equations describing the relationship between soil temperature and soil-moisture content each separately and in combination, as independent variables, and the cumulative rate of CO₂ production were computed. A substantially strong relationship with soil temperature but inconsistent and contradicting results with soil moisture were obtained. Inclusion of soil temperature and moisture in a single model as an independent variable in relation to cumulative CO₂ as a dependent variable improved the soil moisture-CO₂ production relationship.     

Seedling recruitment in a semi-arid steppe: The role of microsite and post-dispersal seed predation

Grasslands dominated by Stipa tenacissima are important ecosystems in the arid and semiarid climates of western Mediterranean, where they have originated by degradation of open dry forests and shrublands. At present, although the level of exploitation in these ecosystems is very low, succession processes seem to have stopped. In this study, we explore the role of microsite and post-dispersal seed predation on the seedling emergence of two species from advanced successional stages (Rhamnus lycioides, Quercus coccifera), three species from degraded stages (Cistus clusii, Helianthemum violaceum, Anthyllis terniflora), and of the dominant species (Stipa tenacissima). Seeds of these species were sown in plots divided into two microsites: (i) under tussocks of S. tenacissima and (ii) in the bare soil between tussocks. Soil moisture was significantly higher in the tussock microsites. The interaction observed between microsite and time, reflected the slower drying process under tussocks. Seed predation was in general high and differed significantly between species but not between microsites. Q. coccifera was especially affected by predation. The only germination detected in gap areas was that of H. violaceum. The results pointed to a direct facilitative effect of tussock grass on the germination in R. lycioides (20.4±8.0%) and S. tenacissima (41.3±5.7%) while the corresponding figures for bare ground were 0%. These results suggest that the recovery of potential vegetation in this ecosystem is not possible or, at least, is very slow because the residual shrub patches do not generate a sufficient number of seeds able to disperse to favorable germination microsites. S. tenacissima may act as facilitator of seedling recruitment if there is a supply of diaspores.     

Germination of Denmoza rhodacantha (Salm-Dyck) Britton & Rose (Cactaceae)

The germination response of Denmoza rhodacantha (Salm-Dyck) Britton & Rose to seed scarification and different calcium concentrations was analysed. Both scarified and unscarified seeds were treated with two different calcium concentrations (1.74 or 6.36 meq l⁻¹ calcium sulfate in distilled water). All treatments were conducted under a constant temperature (30 °C), and a photoperiodic regime of 12 h light and 12 h dark. Germination was initiated significantly sooner (3.6 days), and the rate of final germination was significantly higher (90.8%), for scarified seeds treated with a solution of 6.36 meq l⁻¹ Ca compared with all other treatments (9.0–11.4 days and 3.6–6.8%, respectively). There were no significant differences among the treatments for the time in which 50% of final germination occurred (6.9–13.6 days). The results suggest that both factors, scarification and calcium concentration, favor germination of Denmoza seeds.     

Comparative study of seed germination and growth of Kochia prostrata and Kochia scoparia (Chenopodiaceae) under salinity

Soils of arid regions of Central Asia contain salts of different types that may differentially affect seed germination and plant development. We studied effect of NaCl, Na₂SO₄, 2NaCl + KCl + CaCl₂ and 2Na₂SO₄+K₂SO₄+MgSO₄ on germination of Kochia prostrata and Kochia scoparia seeds under a range of concentrations from 0.5 to 5% and at two constant temperature regimes +22 °C and +6 °C. The observed salt tolerance limit of germination at constant temperature +22 °C for both species was 5-6%, while at low temperature (+6 °C) this limit was 2%. The salt tolerance of young plants (before flowering) was 3% for NaCl. Low concentrations of sulfuric and mixed salts had a stimulating effect on seed germination in K. prostrata. Despite similarity of salt-tolerance limits the studied species showed a significant difference in seed recovery ability, i.e. the ability of ungerminated, salt-soaked seeds to germinate after transfer to fresh water. K. scoparia demonstrated a full germination recovery after seed transfer to distilled water while K. prostrata showed only a partial recovery.     

Germination in tamaulipan thornscrub of north-eastern Mexico

In semi-arid environments plants should be selected to germinate at times most suitable for seedling establishment. Germinability, rate of germination and temperature preference for germination (12 or 28°C constant) were analysed with respect to growth form (grass, forb, woody), longevity and seed size. Short-lived species showed preferential germination at either high or low temperature, whereas long-lived species tended to be indifferent to season. Germinability was >20% for 28 spp., 17 species were <10% and 21 species did not germinate under any circumstances. Growth form or life-span did not influence germination. Woody species germinated rapidly. Germinability and germination rate were positively associated.     

A greenhouse study on root dynamics of cactus pears, Opuntia ficus-indica and O. robusta

Over the last 10 years a great interest in spineless cactus pear was shown in the drier areas in terms of both fresh fruit and fodder production. However, there is a lack of knowledge on quantitative data on root dynamics of these plants needed to fully understand its potential under water limiting conditions. This study aimed at quantifying the effects of water stress on the growth of tap roots, side roots and rain roots of the species Opuntia ficus-indica (L.) Miller (cultivar Morado—green cladode) and O. robusta Wendl. (cultivar Monterey—blue cladode). One-year-old cladodes were planted in root boxes and pots (2002/2003 season) that were kept in the greenhouse at day/night temperatures of 25–30 °C/15–18 °C. Placing the cladodes flat on the soil, more areoles came in contact with the soil and therefore more roots developed in both species with an average of only 3.4% areole complexes not rooting. Each areole complex formed on average 3 roots. The highest daily tap root growth was 42 and 36 mm for O. ficus-indica and O. robusta, respectively. Tap root growth increased in the morning with water stress for both species but decreased in the afternoon. Side root growth increased with water stress, with that of O. robusta more per tap root than O. ficus-indica. O. robusta showed a finer root system than O. ficus-indica. The side roots grew as much as 8 and 5 mm per day for O. ficus-indica and O. robusta, respectively. Whitish rain roots developed on the established roots within the first hour after rewetting the soil and grew for only 3 days. Rain roots grew up to 7 and 5 mm within a day for O. ficus-indica and O. robusta, respectively. Considering all studied aspects of their roots systems, O. robusta appears to be better adapted to drought (less sensitive to water stress) than O. ficus-indica.