Embryo aberrations in sea ice amphipod Gammarus wilkitzkii exposed to water soluble fraction of oil

Offshore oil and gas activities have gained momentum in the European Arctic, raising concerns of the potential impact of oil-related chemicals on the polar marine ecosystem, notably on sea ice communities. Herein, malformations on embryos of the Arctic sea ice amphipod Gammaruswilkitzkii exposed to the water soluble fraction of oil were studied. The females ranged from development stage three to nine. No differences in reproductive stage were observed among the different treatments after 30 days of exposure. Frequency of embryo aberrations was significantly higher in the high-dose compared to controls, indicating that the embryos of G. wilkitzkiii were affected by oil.     

Chronic sublethal effects of the water soluble fractions of no. 2 fuel oils on the marine isopod, Sphaeroma quadridentatum

Two series of experiments were carried out to assess the long-term effects of the water soluble fractions (WSF) of fuel oils on the marine isopod, Sphaeroma quadridentatum. In one experiment, juvenile isopods (one month old) were exposed to sublethal concentrations (0·1% to 15% WSF) of a fuel oil (Baytown, Exxon) at room temperatures of 24 ± 1–6°C for nine months. These isopods survived to maturity and reproduced. However, growth rate was adversely affected at concentration ≥ 3% WSF and fecundity was depressed at concentrations ≥ 1% WSF. Survival of offspring in clean seawater was dependent to a large extent on the history of the previous generation. Offspring from groups exposed to ≥ 1% WSF experienced high mortality (> 70% within five weeks) even in clean seawater. This may imply that a population of Sphaeroma exposed to WSF as low as 0·2 ppm may eventually disappear, although animals can grow to maturity and reproduce at concentrations < 3 ppm.In another experiment, adult Sphaeroma (three months old) were subjected to the WSFs of four fuel oils (Baton Rouge, Baytown, Montana and New Jersey) for one month. Fecundity was depressed at a concentration > 15% WSF, regardless of the kind of fuel oil. On the other hand, the number of young produced bythe surviving females varied with the type of fuel oil for the same level of WSF. This may be attributed to different relative amounts of toxic components present in the WSF.     

Effects of dietary and water-borne oil exposure on larval pacific herring (Clupea harengus pallasi)

Pacific herring (Clupea harengus pallasi) larvae were exposed either directly to the water-soluble fraction (WSF) of crude oil or indirectly via oil-contaminated prey (OCP) (Artemia salina) for <- 28 days, to determine the relative effects of diet and water as routes of contamination. Larvae were affected rapidly by 0·9 ppm WSF, which caused high larval mortality, reduced swimming ability and rapid reductions in feeding rates. Larval length was significantly reduced by 0·7 ppm WSF in 7 days and by 0·3 ppm WSF within 14 days. The WSF exposure also caused similar reductions in larval weights. Highly contaminated prey (6 ppm prey exposure) caused significant mortality, but surviving larvae appeared robust. The OCP did not affect swimming, feeding, or growth. Exposure of larvae was not significantly extended by OCP, which rapidly depurated WSF in clean water (98% in 1 day). Therefore, OCP is probably not an important source of low molecular weight petroleum contamination to larval fish in the marine environment.     

The combined effect of salinity,temperature and oil on the growth pattern of embryos of the killifish, Fundulus heteroclitus Walbaum

Embryos of the estuarine teleost, Fundulus heteroclitus, were exposed during development to different concentrations of the water-soluble fractions (WSF) of a No. 2 fuel oil under various temperature-salinity combinations. Temperatures and salinities were, respectively: 20, 25 or 30°C and 10, 20 or 30%S. The WSF were extracted from No. 2 fuel oil daily and diluted to concentrations of 15, 20 or 25% of the original mixture. Controls were exposed to seawater without WSF at each temperature-salinity combination.The length of eleutheroembryos was markedly decreased by exposure to WSF. Temperature exerted an effect on growth at low WSF concentrations (0 and 15% WSF) but not at higher ones. The two highest WSF concentrations had a predominant effect on growth that was not further modified by physical factor influences. Conversely, as the WSF exposure increased, the yolk diameter increased, indicating a decreased utilisation of yolk material during development. The mean number of vertebrae in the eleutheroembryos decreased at high WSF concentrations and at high temperatures. Morphological abnormalities consisted predominantly of spinal deformations (scoliosis and lordosis). The data indicate that the survivors of chemical toxicity during embryo development exhibit sublethal responses which would reduce their survival potential in later life.     

温度对半滑舌鳎的生长、生化组成和能量收支的影响

研究了不同温度(16,19,22,25,28和31 ℃)对半滑舌鳎(Cynoglossus semilaevis Günther)幼鱼的生长、体成分组成和能量收支的影响.结果表明,随温度的升高,半滑舌鳎幼鱼生长率总体呈现先升高后降低的趋势.其中,在19～25 ℃温度范围内,半滑舌鳎幼鱼的特定生长率相对较高,在16和28 ℃下则有所降低,而在31 ℃下,半滑舌鳎幼鱼特定生长率则显著降低.本研究表明半滑舌鳎幼鱼特定生长率与温度符合二次曲线模型.半滑舌鳎摄食量随温度升高而逐渐增大,但在31 ℃时显著减小,饵料转化率则随温度的升高而降低.鱼体脂肪与能值均随温度升高而降低,蛋白质含量受温度影响不显著.温度对半滑舌鳎的能量收支影响显著,其中,生长能和代谢能主导半滑舌鳎的能量分配,生长能占摄食能比例则随温度的升高而降低,呼吸能占摄食能比例则随温度的升高而升高.本研究表明,半滑舌鳎幼鱼(13～37 g)适宜生长温度范围为18～25 ℃,而不同温度导致的半滑舌鳎摄食量和能量收支的差异可能是温度影响其生长的主要生理生态学机制.     

The effects of a water-soluble petroleum fraction on the neuroid electrical activity of the hydroid coelenterate Tubularia crocea

Several properties of the excitable epithelium of the hydroid coelenterate, Tubularia crocea, are affected by a water-soluble fraction (WSF) of Platform Holly, Monterey Formation, crude oil (100% WSF= 20 ppm total hydrocarbons). In a flowing seawater system, 0·5 ml pulses of 1 WSF cause a significant increase in the epithelial pulse activity, while a 10·min exposure to 0·1% WSF produces a significant increase in the epithelial pulse frequency. Electrophysiological responses to the volatile and non-volatile subfractions are equal, but significantly lower than those to the whole WSF. Behaviorally, the 100% WSF elicits a more vigorous proximal tentacle closure than either its volatile or non-volatile subfraction. However, the closure response to the volatile subfraction exceeds that for the non-volatile component.Of the two epithelial neuroid pulse systems, the hydranth pulse (HP) system is affected to a greater extent than the neck pulse (NP) system. During 10 minutes' treatment with 100% WSF the amplitude of the neck pulses and hydranth pulses declined by 50% and 80%, respectively. The maximum ³H-toluene uptake from 100% WSF also occurs within this period, and sodium and calcium concentrations fluctuate rapidly. These results suggest that a rapid bioaccumulation of hydrocarbons by T. crocea alters membrane permeability, upsetting critical ion gradients which, in turn, alter the firing frequency of the epithelial conducting systems. This study demonstrates that concentrations of WSF approaching environmentally realistic levels (in areas of chronic pollution) alter the epithelial conducting systems that co-ordinate important elements of behavior in Tubularia. This system provides a sensitive neuroid-behavioral assay for aquatic contaminants.     

Simulation of arctic surface radiation and energy budget during the summertime using the single-column model

The surface heat budget of the Arctic Ocean （SHEBA） project has shown that the study of the surface heat budget characteristics is crucial to understanding the interface process and environmental change in the polar region. An arctic single - column model （ARCSCM） of Colorado University is used to simulate the arctic surface radiation and energy budget during the summertime. The simulation results are analyzed and compared with the SHEBA measurements. Sensitivity analyses are performed to test microphysical and radiative parameterizations in this model. The results show that the ARCSCM model is able to simulate the surface radiation and energy budget in the arctic during the summertime, and the different parameterizations have a significant influence on the results. The combination of cloud microphysics and RRTM parameterizations can fairly derive the surface solar shortwave radiation and downwelling longwave radiation flux. But this cloud microphysics parameterization scheme deviates notably from the simulation of surface sensible and latent heat flux. Further improvement for the parameterization scheme applied to the Arctic Regions is necessary.     

美国北极地区能源开发政策演变

随着全球气候变暖,各国对北极能源的开发愈发重视。美国北极地区的能源储量巨大,但鉴于美国的北极战略核心是国家安全、环境保护和国际合作,其能源开发相对滞后。2013年奥巴马政府出台了《北极地区国家战略》,开始对北极能源开发予以特别关注。特朗普政府执政期间,加强了对北极近海油气能源开发的支持力度。2021年拜登总统上任后,美国的北极能源政策以气候变化为核心,意在推动北极地区的绿色发展。美国的北极能源开发始终面临着法律、资金、技术、环保等各种问题的制约,这也决定了美国北极地区能源开发的复杂性和长期性。我国宜通过制定北极能源合作开发战略,重视北极等极寒地区绿色技术的应用和研发,推动与美国在北极地区气候变化和能源转型的合作,深化国际协作与交流,积极参与北极治理等举措,促进我国对北极能源资源的开发利用。     

原油污染对栉孔扇贝抗氧化酶活性的影响

以原油水溶性成分(water soluble fraction of crude oil,WSF)为污染物,采用暴露实验法,研究了栉孔扇贝(Chlamys farreri)鳃和消化腺组织中超氧化物歧化酶(Superoxide Dismutase,SOD)和过氧化氢酶(Catalase,CAT)活性的变化.结果表明,WSF污染下,鳃和消化腺组织SOD和CAT酶活性随暴露时间增加一般表现为降低-升高-降低的趋势,酶活性达到最高的时间随浓度不同而变化.第1天时消化腺SOD在0.08 mg/L浓度下被诱导,而后随时间增加表现为抑制效应;浓度为0.21和0.88 mg/L时消化腺SOD酶活性被抑制,随暴露时间延长而活性增加.暴露时间为4d时,石油烃浓度在0.08和0.88 mg/L时鳃组织SOD酶活性均被抑制,而浓度为0.21 mg/L时被诱导.消化腺和鳃组织SOD可以作为扇贝被污染胁迫的指标.     

A contemporary sediment and organic carbon budget for the Kara Sea shelf (Siberia)

It has recently been realized that the Arctic undergoes drastic changes, probably resulting from global change induced processes. This acts on the cycling of matter and on biogenic elements in the Arctic Ocean having feedback mechanisms with the global climate, for example by interacting with atmospheric trace gas concentration. A contemporary budget for biogenic elements as well as suspended matter for the Arctic Ocean as a baseline for comparison with effects of further global change is, thus, needed. Available budgets are based on the late Holocene sedimentary record and are therefore quiet different from the present which has already been affected by the intense anthropogenic activity of the last centuries.

We calculated a contemporary suspended matter and organic carbon budget for the Kara Sea utilizing the numerous available data from the recent literature as well as our own data from Russian-German SIRRO (Siberian River Run-off) expeditions. For calculation of the budgets we used a multi-box model to simplify the Kara Sea shelf and estuary system: input was assumed to comprise riverine and eolian input as well as coastal erosion, output was assumed to consist of sedimentation and export to the Arctic Ocean. Exchange with the adjacent seas was considered in our budget, and primary production as well as recycling of organic material was taken into account. According to our calculations, about 18.5 × 10⁶ t yr^− 1 of sediments and 0.37 × 10⁶ t yr^− 1 of organic carbon are buried in the estuaries, whereas 20.9 × 10⁶ t yr^− 1 sediment and 0.31 × 10⁶ t yr^− 1 organic carbon are buried on the shelf. Most sources and sinks of our organic carbon budget of the Kara Sea are in the same order of magnitude, making it a region very sensitive to further changes.     

Long-term effects of the water soluble fraction of prudhoe bay crude oil on survival,movement and food search success of the arctic amphipod Boeckosimus (= Onisimus) affinis

The arctic amphipod Boeckosimus (= Onisimus) affinis was exposed to the water soluble fraction (WSF) of Prudhoe Bay crude oil. Animals were constantly exposed or one-time exposed for 3 or 10 days and then removed to clean water. In constant exposure experiments mortality was correlated to the strength of the solution for 6 weeks; afterwards animals in all solutions experienced similar mortality. Food search success lessened for 2 weeks, then gradually increased. Distance moved and time spent moving decreased as the concentration of the WSF increased.Animals one-time exposed to the WSF showed initial mortality rates of 15–20%, with little further mortality. Food search success was lessened relative to the strength of the WSF and duration of exposure, with general recovery within two weeks. Distance moved was decreased, but time spent moving was not. The overall period of testing was four months.     

Declining Arctic Ocean oil and gas developments: Opportunities to improve governance and environmental pollution control

There has been global interest in the exploitation of rich hydrocarbon resources in the Arctic for decades. However, recent low oil prices, a low carbon economy climate agenda, and technical challenges of Arctic oil extraction have curbed interest in these Arctic resources. Despite a recent reluctance to explore and develop an offshore Arctic drilling industry, a resurgence in oil and gas prices could spark renewed interests that could pose unacceptable risks of pollution from oil spills. These risks are further compounded by complex governance and sovereignty issues between circumpolar nations. This paper (i) compares cycles of Arctic hydrocarbon exploration and exploitation activity with global energy prices; (ii) outlines current pollution abatement techniques under pan-Arctic national regulations to identify potential gaps; (iii) describes current international frameworks for Arctic governance to highlight how problems could arise if offshore oil drilling returns to the Arctic and associated spills migrate to international waters; and (iv) provides policy recommendations to aid both national and international policy-makers regarding pollution abatement methods for future Arctic drilling.     

北极地区油气资源的空间分布状况及特征分析

近年来,北极地区油气资源备受瞩目,北极地区油气资源开发利用开展得如火如荼,为北极国家能源产量增长带来了新的契机。文章以北极国家为出发点,采用文献梳理法,系统研究俄罗斯、美国、挪威、加拿大等北极国家在北极地区的油气资源勘探、开发情况;采用统计分析法,分析北极地区待发现油气资源与已发现油气资源的空间分布特征。研究发现,北极地区的油气资源在空间上呈现出极不均衡的特点。伴随着油气勘探技术的突破以及北极航道的逐渐开通,北极国家纷纷加快北极地区油气开发活动的进程,目前北极国家油气开发活动主要在陆地,而未来将逐步迈向北极近海。中国作为北极事物的重要利益攸关方,可以积极与北极国家开展合作,进而参与到北极地区油气资源的开发进程。     

Toxicity of a fuel oil to the eggs of parhyale hawaiensis and amphithoe valida (amphipoda)

Experiments were carried out on the toxicity of a fuel oil to developing embryos and juveniles of two marine amphipods. Parhyale hawaiensis eggs were able to develop from early germinal disc stage (early eggs) to juvenile form in concentrations of 10 (2 ppm) up to 40% (8 ppm) WSF, but deleterious effects were evident in hatching success of eggs and survival of juveniles at concentrations ≥ 10% WSF. The juveniles that hatched from those eggs in higher WSFs had greater mortality than the juveniles that hatched from the eggs exposed to lower WSFs. Survival of juveniles from late eggs (cephalic region differentiated) was also greater than those from early eggs. Amphithoe valida eggs were more sensitive to the WSF of fuel oil than the eggs of P. hawaiensis; < 10% of the test eggs hatched in the 4 ppm WSF compared with 66% of P. hawaiensis eggs at the same concentration. The toxicity of fuel oil to the two amphipod eggs is therefore dependent on the concentration and duration of exposure on the one hand, and on the developmental stages and probably the nature of the egg case on the other.     

Toxicity of Santa Barbara seep oil to starfish embryos: Part 2—the growth bioassay

The asteroid Patiria miniata was used to develop a static bioassay for various crude oil water-soluble fractions (WSF). The criterion used is the size of 48-h exposed embryos. The reasons for choosing this species and the growth criterion are discussed. Experiments show that the body length of 48-h exposed embryos is inversely proportional to hydrocarbon concentration of the WSF and the time of exposure. It is also shown that the toxicity of the WSF decreases with the age of the preparation and that the first 12 h following fertilisation are most sensitive to the effects of WSF.     

北极深海沉积物中细菌和古菌群落结构研究

在北极深海沉积物生态系统中,微生物的群落结构由有机质输入、能量的可用性及其他环境因素决定.然而,全球气候变暖及其导致的冰盖提前融化正在影响微生物的多样性.为描述北极深海沉积物中的微生物群落结构及其与环境因素的相关性,我们利用罗氏454对北极深海沉积物样品的16S rDNA扩增子进行了测序,对细菌和古菌群落的丰富度、成分、结构及其系统发育分类地位进行了描述.硫还原和化能有机营养类是细菌群落中的主要类群;而古细菌群落主要是由微生物的关系最为密切的氨氧化奇古菌门(96.66%)和产甲烷古生菌界(3.21%).这项研究描述了北极极点附近深海沉积物(> 3500米)中的微生物多样性,将为以后研究类似环境中微生物代谢过程和途径等功能分析奠定基础.     

原油WSF对三种海洋鱼类肝微粒体EROD活性的诱导和恢复的比较

研究了实验室条件下原油水溶性组分(WSF)暴露对黑鲷、黄鳍鲷和褐菖鲉肝微粒体EROD活性的剂量-效应,时间-效应和恢复过程。实验结果表明,在剂量诱导实验中,褐菖鲉肝EROD活性在原油WSF浓度为50μg/dm3时呈现生物统计学上的显著差异,而黑鲷和黄鳍鲷肝EROD活性在75μg/dm3时才呈现生物统计学上的显著差异;褐菖鲉肝EROD活性诱导倍数最高,但黑鲷的诱导浓度范围较广。在时间诱导实验中,在40μg/dm3原油WSF暴露下黄鳍鲷肝EROD活性在2 d时首先呈现显著差异;三种鱼肝EROD活性均在第4天达到最高,并呈现显著性变化,此后随着暴露时间的延长而逐渐下降并接近对照组水平。在恢复实验中三种鱼肝EROD活性下降并恢复到对照组水平。研究结果表明:对于石油污染物,黑鲷、黄鳍鲷和褐菖鲉肝EROD活性都可以作为污染生化效应监测指标,然而就三种鱼类比较而言,褐菖鲉最敏感,更适合于作为石油类污染及其生化效应,尤其是低剂量效应的监测生物。     

Toxicity of Santa Barbara seep oil to starfish embryos: Part 1—hydrocarbon composition of test solutions and field samples

The composition and concentration of the water-soluble fraction (WSF) of four oils (Prudhoe Bay Crude, Holly Monterey Crude, Holly Rincon Crude and Isla Vista Seep Oil) are described. Changes in concentration and composition with exposure time in our bioassay chambers are described. Rapid concentration decreases and an unusual compositional relationship point to the importance of performing chemical analyses in conjunction with bioassay experiments.Comparison of the chemical composition of dissolved hydrocarbons in seawater at the Isla Vista Seep Oil site with the WSF of the seep oil shows many similarities, although the concentration is lower than the WSF by approximately 1000-fold.     

巴芬湾西部和格陵兰岛东部大气经向热量输送协同变化对夏季北极海冰的影响

利用欧洲中心气候再分析资料和美国国家冰雪数据中心北极海冰面积资料,分析了夏季北极海冰面积与前期大气经向热量输送年际变化的联系。结果表明：6月北半球中高纬大气的经向热量输送以瞬变热量形式为主,其中巴芬湾西部（B区）和格陵兰岛东部（G区）是瞬变热量向极区传输的两个通道,二者之间存在反位相的协同变化,且这种协同变化与夏季北极海冰面积变化密切相关。可能的机制为：6月,AD、AO和NAO三种北极大气环流型能够引起巴芬湾西部和格陵兰岛东部瞬变热量输送的协同变化,这种协同变化通过涡旋动力作用激发夏季极区大气表现为AD异常,同时影响途经区域的气温,从而通过热动力作用影响夏季北极海冰。将向极区输送的热量称为暖输送,从极区输出的热量为冷输送,则上述两个区域的瞬变热量协同输送可分为三种情况：B暖G冷、B冷G暖、B和G均冷,而B和G均暖的情况十分罕见。当B区向极区输入、G区输出热量时,有利于太平洋扇区和喀拉海的海冰偏少;当G区输入、B区输出热量时,利于喀拉海和拉普捷夫海海冰偏少;当B区和G区均输出热量时,利于波佛特海南部、喀拉海和拉普捷夫海海冰偏多,反之则相反。     

Effects of oil exposure on oxygen consumption of cod eggs and larvae

The oxygen consumption reflects the aerobic energy production of an animal. For fish eggs and larvae the normal oxygen consumption represents the optimal conditions for growth and development. Under the stress of environmental pollution gross deviation in oxygen uptake rate of the developing fish embryo may reflect metabolic disturbances and impede the development. Since the eggs and larvae of cod (Gadus morhua L.) are likely to be exposed to oil pollution from offshore installations in the North Sea we decided to investigate the effects of the water-soluble fraction (WSF) of North Sea crude oil on the oxygen consumption of cod eggs and larvae. The results showed that oxygen consumption of the larvae at the time of final yolk absorption (5–7 days post hatching at 5°C) is strongly suppressed by oil exposure at concentrations down to 50 ppb. No effect on the oxygen uptake, however, was found during the egg stage.