Morphological changes and allometric growth in hatcheryreared Chinese loach Paramisgurnus dabryanus(Dabry de Thiersant, 1872)

The length-weight relationship and allometric growth patterns of hatchery-reared Chinese loach, Paramisgurnus dabryanus(Dabry de Thiersant, 1872), were determined from hatching to 60 days after hatching. A best power model was estimated for the length-weight relationship during the early life stages. Positive allometric growth for the head segment, trunk length, tail length and eye diameter was also found in the early life stages, while body depth, tail depth, tail fi n length, pectoral fi n length and barbel length displayed a negative coeffi cient. During the subsequent early developmental stage, the growth coeffi cients showed a clear and common tendency towards isometry for all measured body ratios. The allometric growth changes in Chinese loach during the early stage are possibly the result of selective organogenesis directed towards survival priorities.     

Ontogenetic development in the morphology and behavior of loach （Misgurnus anguillicaudatus） during early life stages

Loach （Misgurnus anguillicaudatus） is a commercially important fish in China and an ideal aquaculture species. However, culturists experience high larval and juvenile mortality during mass production. To provide insight into ways to improve larviculture techniques, we describe the morphological characteristics and behavior of loach during the larval and early juvenile stages. Yolksae larvae ranged from 2.8 to 4.0 mm body length （BL） between days 0 to 4; preflexion larvae ranged from 3.6 to 5.5 mm BL between days 4 to 6; flexion larvae ranged from 4.8 to 8.1 mm BL between days 5 and 14; and post-flexion larvae ranged from 7. l to 15.7 mm BL between days 11 to 27; the minimum length and age of juveniles was 14.1 mm BL and 23 d, respectively. Loach are demersal from hatch through to the early juvenile stages. A suite of morphological characteristics （e.g., external gill filament and ventral mouth opening） and behavioral traits have developed to adapt to demersal living. We observed positive allometric growth in eye diameter, head length, head height, and pectoral fin length during the early larval stages, reflecting the priorities in the development of the organs essential for survival. Our results provide a basis for developing techniques to improve the survival of larval and juvenile loach during mass production.     

池养条件下军曹鱼早期发育阶段的生长和摄食

研究了军曹鱼(Rachycentron canadum)早期阶段的生长和摄食特性。结果表明:1~29日龄间,全长与日龄之间呈指数函数相关:y=3.6922e0.0995x(R=0.9913);体重与日龄间呈指数函数相关:y=0.0002e0.276x(R=0.9830);体重与全长之间为幂函数相关:y=5×10-6x2.786(R=0.9957);口径与日龄的关系为直线函数关系:y=0.1034x-0.0044(R=0.9939)。鱼苗早期阶段的生长速度存在显著性差异。军曹鱼早期鱼苗在3~6日龄时主要摄食原生动物、单轮幼虫和轮虫;7~12日龄时主要摄食桡足类无节幼体;13日龄以后主要摄食桡足类成体。仔、稚鱼阶段,军曹鱼的摄食活动呈现明显的昼夜节律,其饱满度指数均在12时前后出现最高峰,日摄食率分别为57.36%和14.78%。     

高产池塘白鲢鱼苗鱼种生长的研究

本文对高产养鱼池塘中白鲢鱼苗鱼种阶段的生长特性和规律进行了研究。经统计分析表明:1、白鲢鱼苗鱼种阶段的生长过程中,全长和时间的关系均呈直线相关(L=a bt)。鱼种全长以7-8两月生长最快。日增长率、瞬时增长率以鱼苗阶段居高,鱼种阶段次之。2、白鲢鱼苗阶段体重和时间呈指数相关(W=ae~(bt)),体重生长之速度(dw/dt)和加速度(d~2w/dt~2)与时间成正比。3、鱼种阶段体重与时间呈“S”形曲线相关(W=K/1 ae~(-bt)),体重的生长则以7～9月间最快。生长曲线拐点在t=-1n(1/a)/b;W=k/2。体重生长之速度和加速度分别呈“正态”和“正弦”曲线形。日增重率、瞬时增重率和相对增重率亦以鱼苗阶段较大,鱼种阶段次之。     

黑斑蛙(Rana nigromaculata)早期发育过程中乳酸脱氢酶同工酶

用垂直平板聚丙烯酸胺凝胶电泳技术对黑斑蛙早期胚胎发育过程中乳酸脱氢酶（LDH）同工酶进行研究。结果表明,黑斑蛙胚胎各个不同发育时期LDH同工酶谱有很大差异：LDH1在整个胚胎发育过程中均处于活化状态,LDH2、LDH4在原肠中期开始出现,LDH3在尾芽期开始出现。从尾芽期到尾血循环期只出现LDH－1、－2、－3和-4四条酶带,但不同时期各种同工酶的表达活性不同。     

渤海海峡亚海鲂属鱼类一新记录种——云纹亚海鲂

2012年12月3日,在渤海海峡的蓬莱近海浅水海域捕获雄性成体鱼1尾,该鱼体长34 cm,体高18cm,体长为体高的1.9倍,体宽4.5 cm,头长12.5 cm,体长为头长的2.7倍,尾柄长4.5 cm,体光滑无鳞。管状侧线前半部弧形,后半部平直,行于体中央。棘状骨板沿背鳍基底两侧各12块,臀鳍基底两侧各8块,每一骨板中央具1棘,向后;胸部6块,自第3骨板开始分左右两行;腹部7块,自第2骨板开始分左右两行,胸腹部骨板棘小,形成锯齿缘。背鳍1个,鳍棘部与鳍条部之间有一深缺刻;鳍棘细长,棘间鳍膜延长成丝状,最长超过尾鳍末端。据其形态特征和测量值,鉴定为云纹亚海鲂(Zenopsis nebulosa Temminck et Schlegel,1847),为该海域首次记录。     

Length-weight relationships for six freshwater fish species in Iran

The aim of this study is to report the length-weight relationship parameters (a and b) for some economically important fish species from Iranian freshwaters. The length-weight relationships (LWR) are calculated for six freshwater fish species collected during 2006 and 2007 in Iran. The values of the exponent b in the LWR (W=aLb) vary between 2.985 and 3.543. The LWR with high correlation coefficient (r2) is significant for all the species. These parameters are of great importance to evaluate the relative condition of populations, biology, species management and their fisheries and stock assessment. The application of the length-weight relationships presented here should be limited to the observed length ranges.     

广西条鳅亚科间条鳅属鱼类一新种（鲤形目：爬鳅科）

2011 年8 月在广西靖西县安德乡-洞穴地下水中采到4 尾标本,经鉴定为间条鳅属一新种,命名为小间条鳅Heminoemacheilus parva.新种区别于同属已知种郑氏间条鳅H. zhengbaoshani 的特征为无眼,无鳞,无色透明,侧线完全,胸鳍末端超过胸鳍基部与腹鳍基部之间的中点.区别于同属另一已知种透明间条鳅H. hyalinus 的特征为：体细长,体长为体高的4.8～7.3 倍;头较长,体长为头长的3.1～3.6 倍;尾柄细长,尾柄长为尾柄高的1.6～2.4 倍.     

STUDY ON EMBRYONIC DEVELOPMENT AND EARLY GROWTH OF TRIPLOID AND GYNOGENETIC DIPLOID LEFI—EYED FLOUNDER, PARALICHTHYS OLIVACEUS （T. et S. ）

The early effects of chromosomal manipulation of eggs and sperm on the yields of trip-loid and gynogenefic diploid larvae of Paralichthys olivaceus were investigated. Triploidy was achieved by cold shocking fen.ilized eggs at 0 - 2℃ for 45 minutes duration 5 minutes after fen.ilization, and the in-duced triploidy rates were 31.2% - 50% and the relative hatching rates were 53.3% - 99%. Gynnge-aetic diploids were obtained when eggs were inseminated with irradiated sperm and cold shocked at 0 -2℃ for 45 minutes duration 5 minutes after fertihzation. The induced gynogenetic diploid rates and the relative hatching rates were 94 % - 96 % and 48.5 % - 68.5 % respectively. The embryonic development of the triploid experimental group and of the gynogenetic diploid experimental group was delayed at first compared with the control group. But from the gastrula stage, it was not delayed anymore. There were no significant differences in the growth of the triploid experimental group larvae and the control group larvae, and in the growth of the gynogenetic diploid experimental group larvae and the control group larvae ac-cording to Student‘s t-test (α = 0.05). The relationship between the early growth of the triploid experi-mental group larvae and that of gynogenetic diploid experimental group larvae was also studied.     

勒氏笛鲷Lutjanus russelli Bleeker的年龄和生长研究

采用胸鳍覆盖处的体表鳞片为研究勒笛绸年龄的鉴定材料，判断年轮的依据是以环片切割现象为主，结合环片排列的疏密现象。鳞径（Ｒ）与体长（Ｌ）的关系为Ｌ＝１９．８１５９＋６３．３６９６Ｒ。用ＶｏｎＢｅｒｔａｌａｎｆｆｙ生长方程可表达体长、体重与年龄的关系为Ｌ＝４６９．８［１－ｅ－０．２４２５（ｔ＋０．３７６２）］、Ｗｔ＝３３６６．６［１－ｅ－０．２４２　５（ｔ＋０．３７６　２）］３，生长拐点ｔｒ＝４．１５ａ。作为渔业管理对策，勒氏笛鲷捕捞年龄应定在４龄以上，人工养殖年限以２～３龄最好。     

云南洞穴盲金线鲃一新种(鲤形目:鲤科)

描记采于云南省邱北县一地下洞穴盲金线鲃一新种,以其头上骨质突形状如鹰喙命名鹰喙角金线鲃Sinocyclocheilus aquihornes sp.nov.。新种头形及完全退化的眼与分布于云南泸西阿庐古洞的透明金线鲃S.hyalinus Chen et Yang(1994)相近,但有下列特征可区别:1)角形如鹰喙,后者为一向前的直突起;2)侧线完全,侧线鳞为36,后者侧线不完全,侧线鳞孔9-11;3)背鳍起点位于体中点,至吻端的距离等于至尾鳍基的距离;后者背鳍起点位于体中点之后,至吻端的距离大于至尾鳍基的距离。     

不同条件延缓未受精卵激活对鱼类细胞电融合的影响

泥鳅未受精卵先在合成卵巢液、郝氏液中分别浸泡15、20、30min,然后再受精孵化,统计发育情况,结果表明,合成卵巢液可有效延缓未受精卵遇水后被激活的时间,郝氏液对延缓未受精卵被激活也有一定作用,但效果比合成卵巢液差。用合成卵巢液配制不同浓度胰酶处理受体卵进行细胞电融合实验,胰酶质量浓度为5 mg/mL时,融合卵发育至囊胚期的比例比对照组(郝氏液)高2%,统计分析表明该差异是显著的(P<0.05);但发育至原肠期及孵出期的比例则与对照组无显著差异。胰酶质量浓度为2和1 mg/mL时,融合卵发育到各阶段的比例均低于对照组,统计分析表明两者的差异是显著的(P<0.05)。研究表明,溶液种类、胰酶浓度等对电融合卵的发育有明显的影响,但延缓受体卵的激活时间并不能提高电融合卵的出苗率。用郝氏液配制的2 mg/mL的胰酶溶液处理受体卵,可获得较高比例的融合鱼。     

Histological Study on the Digestive System Development of Takifugu rubripes Larvae and Juvenile

The digestive tract of Takifugu rubripes during early life stages was studied with light microscopy. At the beginning of hatching, the digestive tract is represented by a simple and undifferentiated straight tube and does not communicate with the exterior, as the mouth and anus are not open yet. At 2 d after hatching, a constriction between intestine and rectum that will become the intestino-rectal valve is visible. During the endogenous feeding phase, the yolk sac is resorbed and the digestive tract becomes functional and differentiated. The liver and pancreas also become apparent at this time. At onset of exogenous feeding （3 d after hatching）, yolk sac resesves are not completely depleted, suggesting a period of mixed nutrition. The digestive tract differentiates fully into b.uccopharynx, esophagus, stomach, intestine and rectum. At 9 d after hatching, the yolk sac reserves are completely depleted. The most noticeable events occurring from 5 to 17 d after hatching are the transformation of the epithelia type, the differentiation of the pneumatic sac, the epithelial cell, gut convolution, mucosal fold increase, and the growth of liver and pancreas. From 18 to 27 d after hatching, the numbers of intestine folds and mucus cells increase progressively. From then on, morphological changes of digestive features are almost completed. At 27 d after hatching, the morphology and function of digestive system are similar to that of the adult.     

An integrative study of larval organogenesis of American shad <Emphasis Type="Italic">Alosa sapidissima</Emphasis> in histological aspects

We describe organogenesis at a histological level in American shad (Alosa sapidissima) larvae from 0 until 45 days after hatching (DAH). Larval development was divided into four stages based on the feeding mode, external morphological features, and structural changes in the organs: stage 1 (0–2 DAH), stage 2 (3–5 DAH), stage 3 (6–26 DAH) and stage 4 (27–45 DAH). At early stage 2 (3 DAH), American shad larvae developed the initial digestive and absorptive tissues, including the mouth and anal opening, buccopharyngeal cavity, oesophagus, incipient stomach, anterior and posterior intestine, differentiated hepatocytes, and exocrine pancreas. The digestive and absorptive capacity developed further in stages 2 to 3, at which time the pharyngeal teeth, taste buds, gut mucosa folds, differentiated stomach, and gastric glands could be observed. Four defined compartments were discernible in the heart at 4 DAH. From 3 to 13 DAH, the excretory systems started to develop, accompanied by urinary bladder opening, the appearance and development of primordial pronephros, and the proliferation and convolution of renal tubules. Primordial gills were detected at 2 DAH, the pseudobranch was visible at 6 DAH, and the filaments and lamellae proliferated rapidly during stage 3. The primordial swim bladder was first observed at 2 DAH and started to inflate at 9 DAH; from then on, it expanded constantly. The spleen was first observed at 8 DAH and the thymus was evident at 12 DAH. From stage 4 onwards, most organs essentially manifested an increase in size, number, and complexity of tissue structure.     

2月龄花鲈形态性状与体质量相关性及通径分析

【目的】探明花鲈(Lateolabrax maculatus)早期主要形态性状对体质量的影响程度,为其良种选育提供参考。【方法】采用数理统计方法对500尾2月龄花鲈的全长(X₁)、体长(X₂)、体高(X₃)、头长(X₄)、头高(X₅)、躯干长(X₆)、吻长(X₇)、眼径(X₈)、尾柄长(X₉)、尾柄高(X₁₀)等10个形态性状和体质量(Y)进行测量分析。【结果】相关性分析结果表明,10个形态性状与体质量的相关性均达到了极显著水平(P<0.01)。通径分析结果发现,体高、全长、眼径、躯干长、吻长、体长等6个性状是影响2月龄花鲈体质量的主要形态性状,其中仅体高对体质量的直接作用大于间接作用,为影响体质量的核心变量。决定系数显示,体高对体质量的单独决定系数最大(0.324),全长和体高对体质量的共同决定系数最大(0.287),6个形态性状对体质量的总决定系数为0.947,表明本研究已将影响2月龄花鲈体质量的主要形态性状全部纳入。经逐步回归分析,拟合得到最优线性回归方程为Y=-1.468+1.314X₃+0.160X₁-0.307X₈+0.149X₆+0.305X₇+0.046X₂。【结论】除体质量外,还须将体高作为花鲈早期选育的目标性状。     

Age,growth,and mortality rate of the yellow goosefish Lophius litulon(Jordan,1902) in the Yellow Sea

With the decline in the most fisheries resources in the Yellow Sea,the yellow goosefish Lophius litulon has increased in commercial and ecological importance in recent years.We studied the length distribution,length-weight relationship,age composition,growth pattern,mortality,and exploitation rates of the yellow goosefish in the Yellow Sea.Total length(TL) of females and males ranged from 173 to582 mm and 178 to 500 mm,respectively.The length-weight relationships were also estimated for females and males.Age classes from 2 to 4 years predominated in the samples.The von Bertalanffy growth function(VB GF),estimated based on non-linear least-squares methodology,showed significant differences between sexes.Females attained a greater estimated asymptotic total length(765 mm TL) compared to males(579 mm TL).The VBGF did not differ significantly between stocks of the northern Yellow Sea and the southern Yellow Sea.Estimated natural instantaneous mortality rate(M) ranged from 0.25/a to 0.33/abased on four age-and length-based methods.Total instantaneous mortality rate(Z) of total samples calculated by the age-based catch curve method was 0.591/a and the average fishing mortality(F) was 0.30/a.Estimated exploitation rate(E) was approximately 0.5,indicating that the population of L.litulon in the Yellow Sea may be sustainable.These results provide a reference for the present status of L.litulon and information for the management.     

Yield trait improvement of bay scallops following complete diallel crosses between different scallop stocks

This study describes the complete diaUel hybridization between newly introduced bay scallop stock （W） from Canada and local commercial stock （D） grown under laboratory conditions, in China. Larval survival and growth during all life stages （larvae, spat, and adult） were compared among hybrid （DW, WD） and purebred （DD, WW） populations. Significant heterosis was detected for survival during the larval stage （〉 1% of the mid-parent values）. The mean heterosis （Hm） varied in growth throughout the life span. More than 50% of the Hm values were positive and negative in the DW and WD groups, respectively. The influence of maternal effects and mating types （intrapopulation vs. interpopulation crosses） on growth for all life stages was not consistent. Larval survival did not differ significantly （P〉0.05） with maternal effect or mating type. In the harvest stage, shell length （SL）, shell height （SH）, shell width （SW）, and total weight （TW） were larger in the hybrid compared with the inbred groups. Positive Hm values were observed in SL （1.5%）, SW （5.8%）, and TW （12.3%）, and were more significant in the DW groups （6.1%, 4.5%, 6.8%, and 27.2%）. These results suggest that hybridization between two geographic populations is a good tool for improving bay scallop growth. However, unstable heterosis between the two populations requires further study.     

Ontogeny of the lymphoid organs of Japanese flounder, Paralichthys olivaceus

Histological study on the ontogeny of the lymphoid organs, kidney, thymus and spleen of Japanese flounder, Paralichthys olivaceus, from hatching to 40 d was carried out. The pronephric kidney duct appeared early in hatching although the primordial haemopoietic stem cells were observed within a week after hatching. The spleen was first seen after 8d of hatching. The thymus appeared after 15d, situated near the pronephric kidney. Small lymphoid cells appeared during the later phase of the post-larval stage in the sequence of thymus, kidney and spleen. During the 40d of observations, there were no distinct inner or outer zones in thymus and no red or white pulp in spleen. These results suggest that the nonspecific defense immune system plays a very important role in the early larval stage of Japanese flounder.     

Effects of salinity on egg and fecal pellet production,development and survival,adult sex ratio and total life span in the calanoid copepod, <Emphasis Type="Italic">Acartia tonsa</Emphasis>: a laboratory study

The effects of salinity on the copepod, Acartia tonsa in terms of daily egg production rate (EPR), hatching success, fecal pellet production rate (FPR), naupliar development time and survival, sex ratio, and total life span were determined in laboratory conditions through three experiments. In experiment 1, EPR, hatching success, and FPR of individual females were monitored at salinities of 13, 20, 35 and 45 during short-periods (seven consecutive days). Results show EPR was affected by salinity with the highest outputs recorded at 20 and 35, respectively, which were considerably higher than those at 13 and 45. Mean FPR was also higher in 35 and 20. In experiment 2, the same parameters were evaluated over total life span of females (long-term study). The best EPR and FPR were observed in 35, which was statistically higher than at 13 and 20. In experiment 3, survival rates of early nauplii until adult stage were lowest at a salinity of 13. The development time increased with increasing of salinity. Female percentage clearly decreased with increasing salinity. Higher female percentages (56.7% and 52.2%, respectively) were significantly observed at two salinities of 13 and 20 compared to that at 35 (25%). Total longevity of females was not affected by salinity increment. Based on our results, for mass culture we recommend that a salinity of 35 be adopted due to higher reproductive performances, better feeding, and faster development of A. tonsa.     

新型城镇化的人地耦合异速增长分析方法

在新型城镇化背景下的城市人口规模与用地面积变化具有复杂性,研究两种要素城镇化水平的相对增长关系,有助于探索人地耦合发展的协同态势及变化动因。本文选取广州市2000—2015年的Landsat时序影像与2000、2010和2015年的人口普查与年鉴数据,在街道（乡镇）尺度上计算人口土地城镇化耦合指数识别4种耦合类型,并在此基础上提出年均增长率异速关系拟合方法。研究结果表明：① 2000—2015年广州市街道（乡镇）人口和土地城镇化呈现明显的圈层结构（核心、内、外圈层）特征,4种人地耦合类型的异速增长分别呈现正异速、负异速、负幂律3种形式,证明人地耦合分类的异速增长建模方法有助于分析人地城镇化的动态演化过程;② 基于人地耦合指数的异速标度分析表明,核心圈层—内圈层—外圈层结构上分别形成了高度集约—集约—相对粗放式扩张的发展格局,结合城市更新改造的空间分析,说明这一特色新型城镇化模式在广州具有一定成效,已初步形成了核心圈层与内圈层协同发展的态势,但需要注意防控外圈层的粗放型增长。研究结果可为新型城镇化理论内涵的认知探索和广州市案例的实证研究提供支持。