Physical and chemical properties of soils under some piñon-juniper-oak canopies in a semi-arid ecosystem in New Mexico

Piñon (Pinus edulis)-juniper (Juniperus monosperma)-ecosystems increased substantially in the western USA during the 20th century. Sustainability of these ecosystems primarily depends on soil quality and water availability. This study was undertaken with the objective of assessing the effect of tree species on soil physical quality in a semi-arid region in the western part of Sugarite Canyon, northeast of Raton, Colfax County, NM (37°56′32″N and 104°23′00″W) USA. Three cores and three bulk soil samples were obtained from the site under the canopy of three juniper, Gambel oak (Quercus gambelii) and piñon trees for 0–10 and 10–20 cm depths. These samples were analyzed for particle size distribution, soil bulk density (ρ_b), water stable aggregation (WSA), mean weight diameter (MWD) of aggregates, pH, electrical conductivity (EC) and soil organic carbon (SOC) and total nitrogen (TN) concentrations and stocks. Sand content was greater under juniper (48%) than oak (32%), whereas clay content followed the opposite trend. The ρ_b, WSA, MWD, pH and EC were similar under juniper, piñon, oak canopies for both depths. Estimated (from Philip and Green and Ampt infiltration models) and measured water infiltration parameters did not vary among these sites and were in accord with the values for ρ_b, WSA and MWD. The SOC concentrations and stocks were greater under oak (43.1 Mg ha⁻¹ for 0–10 and 37.5 Mg ha⁻¹ for 10–20 cm depths) than piñon (23.3 Mg ha⁻¹ for 0–10 and 18.5 Mg ha⁻¹ for 10–20 cm depths). The TN concentrations were greater under oak (3.4 g kg⁻¹) than piñon (1.7 g kg⁻¹) for the 0–10 cm depth only. Accumulation of detritus material under tree canopies reduced soil compaction and crusting caused by raindrop impact and increased SOC, and TN concentrations, and water infiltration. Coefficients of variation ranged from low to moderate for most soil properties except infiltration rate at 2.5 h, which was highly variable. Overall, soil quality for each site was good and soil aggregation, water infiltration and SOC concentrations were high, and soil ρ_b was low.     

Influence of understory removal, thinning and P fertilization on N2fixation in a mature mesquite (Prosopis glandulosavar.glandulosa) stand

The natural abundance¹⁵N/¹⁴N method was used to estimate the influence of silvicultural and P fertilization treatments on N accretion, N₂fixation and N partitioning among tissues in a mature mesquiteProsopis glandulosavar.glandulosastand in Texas. The silvicultural treatments consisted of understory removal, herbicide treatment of brushy resprouts, thinning trees to single stems and 100 kg ha⁻¹P fertilization. The trees had a mean basal diameter of 17·8 cm with 8 to 35 cm range. The stand was slow growing with the increase in dry matter ranging from 0·465 Mg ha⁻¹year⁻¹to 0·701 Mg ha⁻¹year⁻¹for the 8 years after the treatments were applied. N accretion after 8 years ranged from 3·1 kg ha⁻¹year⁻¹to 4·4 kg ha⁻¹year⁻¹.Due to the range in δ¹⁵N of the leaves, twigs, branches and trunk, we used the weighted (by biomass) average δ¹⁵N per tree in calculations of the percent N derived from N₂fixation (%Ndfa). There was considerable variability in δ¹⁵N of the reference plants, i.e. from 3·3 to 5·9. In contrast there was low variability in the background δ¹⁵N of nearby soils (7·0±1·0). As the total above-ground biomass δ¹⁵N of a grass grown outside the influence of mesquite (7·8±0·58) had the same δ¹⁵N as the soil (7·5±1·0), we used the grass outside the influence of mesquite and the weighted tree mean δ¹⁵N to calculate % of N derived from N₂fixation.The decrease in intraspecific competition by thinning multistemed trees to single stemmed trees was the only treatment that significantly (p= 0·0001) increased growth. Interspecific competition, i.e. understory removal, did not increase growth. There were no significant differences in total N production or N fixation among treatment means. The most striking result was the highly positive correlation between tree δ¹⁵N and total N per tree and biomass per tree (R²= 0·90,F= 164·4, df. = 18, mean square error (MSE) = 0·155,p= 0·0001). This implies that the younger trees colonizing infertile soils relied more heavily on N₂fixation than larger trees which accumulated 1200 kg ha⁻¹more N under their canopies. The percentage N derived from N₂fixation ranged from 63 to 73% in the various treatments. Despite the high percentage of N derived from N₂fixation, the N₂fixation of the stand was very low, i.e. 1·98 to 2·80 kg N ha⁻¹year⁻¹, due to the low growth of the stand. We believe that comparisons of the whole tree weighted δ¹⁵N to background soil δ¹⁵N provides a more reasonable approach to estimate % N₂fixation than comparisons of leaves of fixers and reference plants.     

Seed germination of five mountain steppe species of Central Asia

This paper presents data on the germination traits of five perennials (Allium polyrrhizum, Agropyron cristatum, Arenaria meyeri, Artemisia frigida and Artemisia santolinifolia) widespread in the mountain steppes of southern Mongolia. Germination and seed viability were assessed at three alternate temperatures (8/4 °C, 20/10 °C, 32/20 °C), three levels of osmotic stress (deionized water; −0.5 MPa, −1 MPa Mannitol solution), and under conditions of alternate light/darkness versus complete darkness. The results of a factorial ANOVA with treatments and species as main effects showed that all five species germinated best at higher temperatures, with only Agropyron cristatum showing some seed mortality. Osmotic stress reduced seed viability and total germination in all five species. Darkness had no influence on viability, but positively affected seed germination of Allium polyrrhizum and Agropyron cristatum. We therefore conclude that, in the field, germination of all five species is mainly controlled by ambient temperatures and water availability, both of which drop towards the end of summer when dispersal takes place and effectively delay seedling recruitment until the next vegetation period.     

Effects of indole-3-acetic acid and zinc on the growth, osmotic potential and soluble carbon and nitrogen components of soybean plants growing under water deficit

Three-week old soybean (Glycine max) plants were subjected to a factorial combination of four regimes of soil matric water potential (ψ_m=−0·03, −0·5, −1·0 and −1·5 MPa), two levels of supplementary Zn (O and 20 mgl⁻¹) and two levels of foliar IAA application (O and 10 mgl⁻¹). Under control conditions (no Zn, no IAA), increasing soil drying progressively retarded shoot and root growth (length and dry mass production), reduced leaf relative water content (RWC) and decreased the contents of chlorophyll (Chl) and shoot soluble sugars (SS), but increased soluble sugar content of roots and lowered osmotic water potential of shoots and roots (osmotic adjustment). Total free amino acid (TAA) content increased in shoots but decreased in roots whereas contents of soluble proteins (SP) decreased in shoots and roots. The effect of water stress was statistically significant (p<0·05) and had a major effect (as indicated by η²values) on leaf RWC, shoot and root dry masses and osmotic potential. Supplementary Zn improved root growth at all levels of stress and shoot growth under severe stress. Improvement of growth was positively correlated with the internal tissue Zn concentrations (r=0·91 and 0·86 for shoot and 0·94 and 0·82 for root length and dry mass respectively). Exogenous IAA raised (p<0·05) RWC, Chl, DM (slightly), root SS, and SP, whereas shoot TAA was lowered. Effects on root TAA and shoot SS were more complex: they were lowered at zero stress and raised under severe stress. IAA and Zn in combination had additive effects on Chl, growth and osmotic potential, but their combined effects on SP and TAA were more complex. It is concluded that the treatment of soybean plants grown under conditions of low soil water potentials and Zn deficiency with Zn and IAA solutions counteracted the deleterious effects of stress, especially at high stress levels, and helped stressed plants to grow successfully under these adverse unfavourable conditions.     

Changes in ecosystem carbon stocks following grassland afforestation of semiarid sandy soil in the southeastern Keerqin Sandy Lands, China

The ecological consequences of grassland afforestation in arid/semiarid sandy regions are not well known with respect to tree species and stand age. The present study quantifies the changes in above- and belowground carbon (C) stocks following afforestation in the southeastern Keerqin Sandy Lands with species of Mongolian pine and poplar. We studied 15-, 24-, and 30-year-old Mongolian pine plantations, 7-, 11-, and 15-year-old poplar plantations, and adjacent grasslands. The results show that total ecosystem C stocks increased following grassland afforestation. Aboveground C stocks increased at a rate of 2.75 Mg C ha⁻¹ yr⁻¹ in the poplar plantations, and 1.06 Mg C ha⁻¹ yr⁻¹ in the Mongolian pine plantations. Mineral soil C stocks decreased during the early stage of forest establishment, but recovered with increasing stand age. Root C stock increased significantly in the Mongolian pine plantations, but the poplar plantations showed no such increase relative to the grassland. Our results indicate that afforestation of the grassland in the southeastern Keerqin Sandy Lands would sequester more C than would continuous grassland. Tree species selection and stand developmental age should be considered in planning future afforestation projects.     

Horizontal and vertical zones of influence for root systems of four Mojave Desert shrubs

Horizontal and vertical zones of influence for root systems of four Mojave Desert shrubs were characterized using ³²P as a nutrient tracer. Larrea tridentata's horizontal zone of influence was sparse near the plant's stem base, with a maximum probability of accessing ³²P (P_max) of 41%. However, its horizontal zone of influence extended beyond 5 m, and the distance from the stem base at which the probability of accessing ³²P was half P_max (L₅₀3 m) was significantly greater than the other three shrubs. Ambrosia dumosa's zone of influence was dense near the plant's stem base (P_max78%), but was rare at distances >2 m (L₅₀1 m). Zones of influence for Lycium andersonii and Lycium pallidum were intermediate between those of L. tridentata and A. dumosa. For vertical zones of influence, L. tridentata was more likely to obtain ³²P from 5 m soil depths than A. dumosa, but L. pallidum was not significantly different from either A. dumosa or L. tridentata. Horizontal zones of influence did not change with treatments that altered soil water and nitrogen availability, but vertical zones of influence increased with a flood irrigation treatment that increased water availability to 5 m soil depth. These differences among species likely reflect compromises between their shoot growth strategies and their need to acquire spatially and temporally limited soil resources, especially through competitive interactions.     

Carbon accumulation, nitrogen and phosphorus use efficiency of Sophora davidii seedlings in response to nitrogen supply and water stress

A greenhouse experiment was conducted to explore whether additional nitrogen (N) supply could enhance carbon (C) accumulation, and phosphorus (P) use efficiency (NUE_P) of Sophora davidii seedlings under dry conditions. Two-month-old seedlings were subjected to a completely randomized design with three water (80, 40 and 20% water field capacity (FC)) and three N supply (N0: 0, Nl: 92 and Nh: 184 mg N kg⁻¹ soil) regimes. Water stress decreased C, N and P accumulation, NUE_P, N and P uptake efficiency (NUtE_N and NUtE_P) regardless of N supply. The S. davidii seedlings exhibited strong responses to N supply, but the responses were not consistent with the various N supply levels. Nl increased C, N and P accumulation, and improved NUE_P, NUtE_N and NUtE_P in the same water treatment. In contrast, Nh did few or even depress effects on C, N and P accumulation, and NUE_P, although NUtE_N and NUtE_P increased with Nh in the same water treatment. Even so, NUE_N decreased with increase of N supply in the same water treatment. The results suggested that appropriate or low N supply should be recommended for S. davidii seedling establishment in dry environment by improving C accumulation and NUE_P.     

Soil seed flora, germination and regeneration pattern of woody species in an Acacia woodland of the Rift Valley in Ethiopia

The objectives of this study were to determine the composition, density and spatial distribution of the soil seed bank of woody species, as well as their regeneration pattern in two different land use systems, controlled (ranch) and open grazing, in an Acacia woodland of the Rift Valley in Ethiopia. We also compared the species composition of the soil seed bank and the above-ground vegetation to find out if differences exist in the soil seed bank and advance regeneration between the two land use systems. The germination requirements of seeds of the woody species were also investigated under laboratory conditions. Acacia senegal, Acacia seyal, Acacia tortilis, Dichrostacys cinerea and Balanites aegyptiaca were encountered in the above-ground vegetation in both systems. Seeds of only A. tortilis (90±32 seeds m⁻²) from the ranch, and A. senegal (5±3 seeds m⁻²) and A. tortilis (72±34 seeds m⁻²) from the open system were found in the soil seed bank along transects with patchy horizontal pattern. The two systems were not significantly different in density of soil seed banks of A. senegal andA. tortilis . Jaccard's similarity index showed that only a few woody species were common in the soil seed flora and above ground vegetation. However, all of the species accumulated seeds (58±43–331±130 seeds m⁻²) in/on the soil under the canopy. Very large numbers of seeds of A. tortilis (19382±9722 seeds m⁻²) and D. cinerea (1278±494 seeds m⁻²) were also found in barns. Most of the seeds recovered from the soil samples (60–80%) were found in the litter layer. Acid and mechanical scarification improved legume germination (36–99% and 60–99%, respectively) over boiling water (0–48%). Treatment means differed significantly for all the legumes (p<0·001) but not for Balanites. Height and diameter class distribution of regeneration of A. tortilis and D. cinerea in both systems and A. senegal in the open system had a negative exponential correlation (r_s=−0·5, −0·25 and −0·86, respectively). A. seyal and B. aegyptiaca showed poor regeneration. Horizontal distribution of advance regeneration of all the species was patchy. Advance regeneration of A. seyal, A. tortilis and B. aegyptiaca were not significantly different, while that of A. senegal and D. cinerea were significantly different between the two systems. Poor representation of species in the soil seed bank along transects and in the different height and diameter classes may be attributed to the low density of mature trees as well as the mode and strategy of seed dispersal. Ungulate and wind dispersed species (e.g. A. senegal, A. tortilis and D. cinerea) were highly favoured. Patchiness in the distribution of seeds and advance regeneration was also a result of endozoochory. Dispersal of non-ungulate dispersed seeds (A. senegal, A. seyal and B. aegyptiaca) was restricted to the canopy zone. Piles of seeds ofA. tortilis and D. cinerea that were found in barns were a result of consumption of their pods by cattle. High concentration of seeds in the litter layer may be due to low soil disturbance and larger size of seeds. The height and diameter class distribution of A. senegal (in the open system), A. tortilis and D. cinerea also indicated that the species have good regeneration. Results from the germination tests indicated that seeds of the legumes require pre-sowing treatments to give a rapid, uniform and improved germination. Intervention through artificial regeneration should be employed to improve the density and regeneration capacity of those species with hampered regeneration at both systems.     

Growth response of three globose cacti to radiation and soil moisture: An experimental test of the mechanism behind the nurse effect

Cactus seedlings often establish under nurse plants which modify environmental conditions by increasing moisture and decreasing solar radiation, which may cause beneficial and detrimental effects, respectively, on seedling growth. Three soil moisture treatments (5%, 25% and 60%) and two solar radiation levels (100% exposure=243 μmol m⁻² s⁻¹, and 40%=102 μmol m⁻² s⁻¹) were used in a factorial design to analyze seedling growth response of three rare cactus species (Mammillaria pectinifera, Obregonia denegrii and Coryphantha werdermannii). The variables evaluated were relative growth rate (RGR), root/shoot ratio (R/S), and K (RGR_roots/RGR_shoot), obtained from an initial seedling harvest (6-month-old seedlings) and a final harvest 6 months after treatment application. All three species had slow RGRs (0.002–0.012 g g⁻¹ day⁻¹). O. denegrii had the lowest RGR values, but was the only species for which R/S and K varied with soil moisture. While all seedlings responded markedly to soil moisture, no response was observed to radiation treatments. The latter might have been related to the relatively low solar radiation levels present in the greenhouse. Yet, our results suggest that the main benefit nurse plants offer to seedlings is the increase in soil moisture.     

Using hyperspectral imagery to predict post-wildfire soil water repellency

A principal task of evaluating large wildfires is to assess fire's effect on the soil in order to predict the potential watershed response. Two types of soil water repellency tests, the water drop penetration time (WDPT) test and the mini-disk infiltrometer (MDI) test, were performed after the Hayman Fire in Colorado, in the summer of 2002 to assess the infiltration potential of the soil. Remotely sensed hyperspectral imagery was also collected to map post-wildfire ground cover and soil condition. Detailed ground cover measurements were collected to validate the remotely sensed imagery and to examine the relationship between ground cover and soil water repellency. Percent ash cover measured on the ground was significantly correlated to WDPT (r = 0.42; p-value < 0.0001), and the MDI test (r = − 0.37; p-value < 0.0001). A Mixture Tuned Matched Filter (MTMF) spectral unmixing algorithm was applied to the hyperspectral imagery, which produced fractional cover maps of ash, soil, and scorched and green vegetation. The remotely sensed ash image had significant correlations to the water repellency tests, WDPT (r = 0.24; p-value = 0.001), and the MDI test (r = − 0.21; p-value = 0.005). An iterative threshold analysis was also applied to the ash and water repellency data to evaluate the relationship at increasingly higher levels of ash cover. Regression analysis between the means of grouped data: MDI time vs. ash cover data (R² =0.75) and vs. Ash MTMF scores (R² = 0.63) yielded significantly stronger relationships. From these results we found on-the-ground ash cover greater than 49% and remotely sensed ash cover greater than 33% to be indicative of strongly water repellent soils. Combining these results with geostatistical analyses indicated a spatial autocorrelation range of 15 to 40 m. Image pixels with high ash cover (> 33%), including pixels within 15 m of these pixel patches, were used to create a likelihood map of soil water repellency. This map is a good indicator of areas where soil experienced severe fire effects—areas that likely have strong water repellent soil conditions and higher potential for post-fire erosion.     

A large daylight geodesic dome for quantification of whole-ecosystem CO2 and water vapour fluxes in arid shrublands

We designed, constructed, calibrated and field-tested a lightweight (30 kg), 4.2 m diameter, 16.4 m³ polyethylene-covered dome static chamber ecosystem gas exchange cuvette that can quantify ecosystem CO₂ and water vapour fluxes as low as 0.1 μmol CO₂ m⁻² s⁻¹ and 0.1 mmol H₂O m⁻² s⁻¹ with little impact on environmental conditions. Fluxes measured in May 2001 in an intact Great Basin sagebrush ecosystem at midday were significantly higher than in an adjacent post-wildfire successional ecosystem, with observed ranges from –0.71 to 1.49 μmol CO₂ m⁻² s⁻¹ for CO₂ and from –0.09 to 0.53 mmol H₂O m⁻² s⁻¹ for water vapour.     

Growth and water relation parameters in drought-stressed Coriaria nepalensis seedlings

In the present study, growth and water relation parameters were analysed in drought-stressed Coriaria nepalensis Wall. seedlings. C. nepalensis seedlings were subjected to four drought cycles of 7, 14, 21, and 28-days, and to one control level (watered on alternate days) in a glasshouse. The seedlings failed to survive a 28-days drought during summer. Osmotic adjustment (defined as the decrease in osmotic potential at zero or full turgor in response to water deficit) was measured as the difference between the osmotic potential of seedlings watered on alternate days (control) and those subjected to 21-days drought cycle. Seedlings subjected to 21-days drought had a predawn water potential of −2.60 MPa, and showed an osmotic adjustment of −1.95 MPa at full turgor and −2.17 MPa at zero turgor. The growth of seedlings was positively related to moisture and with water potential. With decline in soil moisture the root:shoot ratio increased while leaf weight ratio decreased. Leaf characteristics, such as leaf number, leaf area, leaf area ratio, specific leaf area and leaf drop, were also affected by moisture stress. This study has indicated that osmotic adjustment is a major adaptive mechanism of C. nepalensis that aids successful regeneration of seedlings in degraded sites with inhospitable soil conditions.     

Cocoons and hatchlings ofAporrectodea caliginosa(Savigny 1826) (Oligochaeta: Lumbricidae) in Benghazi, Libya

Field and laboratory studies were conducted during the period May 1993–April 1994 on the cocoons and hatchlings ofAporrectodea calignosa(Savigny 1826), a common earthworm found in Benghazi, Libya. Cocoon production started in August 1993 and continued until April 1994. A positive correlation between soil moisture (r=0.74;p< 0.05) and cocoon production, and a negative one between soil temperature (r=−0.85;p< 0.05) on cocoon production were found. Mean incubation time was 47 days. Some cocoons contained a single worm (single hatchling) whereas the majority of them (68%) contained two, the first hatchling and second hatchling. The rate of change of body mass (F=2.18;p>0.05) and the average body mass (F=1.25;p>0.05) of the single, first and second hatchlings were similar.     

Wind erodibility of major soils in the farming-pastoral ecotone of China

Wind erosion and desertification are severe problems in China's farming-pastoral ecotone. In this study, wind erodibility of five major soils in both uncultivated and simulated cultivated conditions, were determined through wind tunnel tests at nine wind speeds ranging from 10 to 26 m s⁻¹. The average wind erosion rate (g m⁻² min⁻¹) under the uncultivated condition (q₀) for the five soils could be set in the order: chestnut soil (28.5)>brown soil (24.8)>sierozem (21.8)>chernozem (19.9)>fixed sandy soil (11.4). The highest natural wind erosion might take place in the semi-arid steppe zone where the Chestnut soils predominate. Cultivation can significantly accelerate wind erosion, the mean wind erosion rate under the cultivated condition (q_c) for all five soils was 743.7 g m⁻² min⁻¹ in the following order: sandy soil (3313.2)>brown soil (227.2)>chernozem (221.8)>sierozem (85.1)>chestnut soil (81.2). For both the uncultivated and cultivated soil samples, the relationship between wind erosion rate (q) and wind speed (U) could be expressed in general as q=A e^BU (A and B are constant coefficients). There was a critical wind speed for each soil type except for the sandy soil. Below the critical wind speed, cultivation reduced wind erosion rate possibly due to soil clodiness and roughness effects. Above the critical speed, cultivation greatly intensified wind erosion rates due to the break down of the original soil structure. The critical wind speed measured at 20 cm above the soil surface was 20 m s⁻¹ for the brown soil, 14 m s⁻¹ for chernozem and the chestnut soils, and 10 m s⁻¹ for the sierozem. Among the five tested soils, the high wind erosion rate of the cultivated sandy soil showed its extreme sensitivity to cultivation, possibly because of the structureless nature of the loose sand. The “effect of cultivation on wind erosion” index, η (=q_c/q₀), increased exponentially with the increase of wind speed, indicating that under higher wind speed conditions, cultivation could result in more severe wind erosion.     

Effect of plant species on shrub fertile island at an oasis–desert ecotone in the South Junggar Basin, China

Shrub fertile islands are a common feature in arid ecosystems. To examine the effect of plant species on the spatial patterns of soil chemical and physical properties surrounding individual shrubs, two deciduous shrub species with different morphologies (Tamarix spp. and Haloxylon ammodendron Bge.) were studied at an oasis–desert ecotone in South Junggar Basin. Soil samples were collected under the shrub crown (canopy), at the vertically projected limit of shrub crown margin (periphery), and in the space between shrub crowns (interspace) at two depths, 0–10 and 10–20 cm, to analyze their physical and chemical properties. The results show that the fertile islands of Tamarix spp. are enriched with more soil nutrients (significantly higher, P<0.05; soil organic matter (SOM); total nitrogen (TN) and available nitrogen (AN); to a deeper depth (>20 cm) and in a larger area (beyond the canopied area) compared to that of H. ammodendron (significantly higher, P<0.05, soil nutrients detected only for AN; <20 cm in depth; smaller than the canopied area). Soil texture patterns surrounding the shrubs of the two species are even more different, with more coarse particles under the Tamarix spp. canopies compared to the interspace between shrubs but fewer under the H. ammodendron canopies compared to the interspaces. These variations are attributed to the difference in morphology of the two studied species: the Y-shaped crowns of H. ammodendron are less capable of capturing and maintaining litter under them than the hemispheroidal crowns of Tamarix spp., which leads to the less well developed fertile islands surrounding H. ammodendron shrubs.     

Diatom-based models for reconstructing past water quality and productivity in New Zealand lakes

The trophic status of lakes in New Zealand is, on average, low compared to more densely populated areas of the globe. Despite this, trends of eutrophication are currently widespread due to recent intensification in agriculture. In order to better identify baseline productivity and establish long-term trends in lake trophic status, diatom-based transfer functions for productivity-related parameters were developed. Water quality data and surface sediment diatom assemblages from 53 lakes across the North and South Islands of New Zealand were analysed to determine species responses to the principal environmental gradients in the data set. Repeat sampling of water chemistry over a 12-month period enabled examination of species responses to annual means as well as means calculated for stratified and mixed periods. Variables found to be most strongly correlated with diatom species distributions were chlorophyll a (Chl a), total phosphorus (TP), dissolved reactive phosphorus (DRP), ionic concentration (measured as electrical conductivity (EC)) and pH. These variables were used to develop diatom-based transfer functions using weighted averaging regression and calibration (simple, tolerance down-weighted and with partial least squares algorithm applied). Overall, models derived for stratified means were weaker than those using annual or isothermal means. For specific variables, the models derived for the isothermal mean of EC (WA-tol r²_jack = 0.79; RMSEP = 0.15 log₁₀ S cm^–1),the annual mean of pH (WA r²_jack = 0.72; RMSEP = 0.25 pH units) and the isothermal mean of Chl a (WA r²_jack = 0.71; RMSEP = 0.18 log₁₀ mg m^–3 Chl a) performed best. The models derived for TP were weak in comparison (for the annual mean of TP: WA r²_jack = 0.50; RMSEP = 0.24 log₁₀ mg m^–3 TP) and residuals on estimates for this model were correlated with several other water quality variables, suggesting confounding of species responses to TP concentrations. The model derived for the isothermal mean of DRP was relatively strong (WA-tol r²_jack = 0.78; RMSEP = 0.17 log₁₀ mg m^–3 DRP); however, residual values for this model were also found to be strongly correlated with several other water quality variables. It is concluded that the poor performance of the TP and DRP transfer functions relative to that of the Chl a model reflects the coexistence of nitrogen and phosphorus limitation within the lakes in the data set. In spite of this, the suite of transfer functions developed from the training set is regarded as a valuable addition to palaeolimnological studies in NewZealand.     

The influence of floodplain geohydrology on the distribution of Sarcocornia pillansii in the Olifants Estuary on the West Coast, South Africa

The distribution of Sarcocornia pillansii (Moss) A.J. Scott was determined by water-table depth and electrical conductivity (EC) of the groundwater. Where the groundwater was accessible (<1.5 m) and had a low EC (<80 mS cm⁻¹), S. pillansii extended its roots down to the water-table where a suitable water potential gradient was shown to exist between the soil and roots. In areas where the groundwater was too deep and/or hypersaline, the plants grew on hummocks. The unconfined aquifer below the floodplain is linked to the estuary and although diurnal tidal waves were dampened, water-table level fluctuations were recorded between tidal events. The complex geomorphology of the floodplain influences groundwater flow, in turn affecting the distribution of the salt marsh vegetation.     

Responses of photosynthesis and water relations to rainfall in the desert shrub creosote bush (Larrea tridentata) as influenced by municipal biosolids

Responses of photosynthesis (P_n), stomatal conductance (g_s), pre-dawn leaf water potential (Ψ_lp) and leaf water content (ω_l) of creosote bush to 10 rainfall events in the Chihuahuan Desert were investigated. Infiltration of rainwater was manipulated by applying municipal biosolids. The responses of P_nand water relation parameters to rainfall (>10 mm) were mainly dependent upon drought severity: (1) following a moderate drought, P_n, g_s, Ψ_lpand ω_lrecovered to corresponding values of irrigated plants within 2 days after a 23-mm rainfall; (2) Ψ_lpand g_sresponded to a 15-mm rainfall within 2 days, following a 25-day drought, whereas responses of P_nand ω_lwere delayed for several days; (3) responses of P_n, g_s, Ψ_lpand ω_lto a 14·7-mm rainfall were all delayed for several weeks following a 110-day drought, but the delay was longer in P_n, g_sand ω_lthan in Ψ_lp. Creosote bush responded to small rainfall events (approximately 6 to 8 mm) with an increase in Ψ_lp, but without noticeable changes in g_sand P_n, suggesting a strong stomatal control of water loss even though xylem embolism was reduced. Biosolids applied at high rates (3·4 and 9 kgm⁻²) decreased the soil water by 2 to 4 mm following rainfall events, and this in turn delayed and decreased the responses of P_nand water relation parameters to rainfall.P_nand g_swere linearly related to ω_land exponentially related to Ψ_lp. With the generally coincidental responses of P_nor g_sand ω_lto rainfall, we concluded that the responses of P_nand g_sto rainfall were dependent on leaf rehydration which resulted from restored hydraulic conductance following drought.     

Salt tolerance of Microcoleus vaginatus Gom., a cyanobacterium isolated from desert algal crust, was enhanced by exogenous carbohydrates

Microcoleus vaginatus isolated from a desert algal crust of Shapotou was cultured in BG-11 medium containing 0.2 mol l⁻¹ NaCl or 0.2 mol l⁻¹ NaCl plus 100 mg l⁻¹ sucrose, extracellular polymeric substances (EPS) or hot water-soluble polysaccharides (HWP), respectively. Photosynthetic oxygen evolution rates, photosystem II activity (Fv/Fm) and dark respiration of NaCl-stressed cells were enhanced significantly by the added sucrose or EPS under salt stress conditions (0.2 mol l⁻¹ NaCl). Compared with cells treated with salt alone, sodium contents in cells reduced significantly; the content of cellular total carbohydrate did not change, and intracellular sucrose, water-soluble sugar increased significantly following the addition of exogenous carbohydrates. Sucrose synthase (SS) activity of NaCl-stressed cells increased following the addition of sucrose, and sucrose phosphate synthase (SPS) activity of NaCl-stressed cells increased following the addition of exogenous sucrose, EPS or HWP compared with cells stressed with NaCl only. The results suggested that the extruded EPS might be re-absorbed by cells of M. vaginatus as carbon source, they could increase salt tolerance of M. vaginatus through the changes of carbohydrate metabolism and the selective uptake of sodium ions.     

Prescribed fire in a Great Basin sagebrush ecosystem: Dynamics of soil extractable nitrogen and phosphorus

Pinyon and juniper have been expanding into sagebrush (Artemisia tridentata) ecosystems since settlement of the Great Basin around 1860. Herbaceous understory vegetation is eliminated as stand densities increase and the potential for catastrophic fires increases. Prescribed fire is increasingly used to remove trees and promote recovery of sagebrush ecosystems. We quantified the effects of prescribed fire, vegetation type, and time following fire on soil KCl extractable nitrogen and NaHCO₃ extractable phosphorus in a pinyon–juniper woodland and its associated sagebrush ecosystem immediately before and for 4 years after a spring prescribed burn. Potassium chloride extractable NH₄⁺ and total inorganic-N increased immediately following prescribed fire, and extractable NO₃⁻ decreased immediately after the burn. In the surface layer (top 8 cm), extractable NH₄⁺ remained elevated compared to the control through year 2 after the burn. By the first fall post-burn extractable NO₃⁻ and total extractable inorganic-N increased and remained elevated over the control through year 3 after the burn in the surface layer. For the entire soil profile (52 cm), the burn had no effect on NH₄⁺, and the effects on total extractable inorganic-N were no longer significant after year 1. However, NO₃⁻ remained elevated over the control through year 2 post-fire for the soil profile. Near surface NaHCO₃ extractable ortho-P increased immediately following fire, and remained elevated through year 2 post-fire. No fire effects were observed for extractable ortho-P in deeper horizons. Our data show that plant available nitrogen can remain elevated for extended periods following prescribed fire. This can influence regrowth and seedling establishment of native plant species, invasion of exotic plant species and, ultimately, site recovery potential.