Identification and bioinformatics analysis of micro RNAs from the sporophyte and gametophyte of Pyropia haitanensis

Pyropia haitanensis(T.J.Chang et B.F.Zheng) N.Kikuchi et M.Miyata( Porphyra haitanensis) is an economically important genus that is cultured widely in China.P.haitanensis is cultured on a larger scale than Pyropia yezoensis,making up an important part of the total production of cultivated Pyropia in China.However,the majority of molecular mechanisms underlying the physiological processes of P.haitanensis remain unknown.P.haitanensis could utilize inorganic carbon and the sporophytes of P.haitanensis might possess a PCK-type C 4-like carbon-fixation pathway.To identify micro RNAs and their probable roles in sporophyte and gametophyte development,we constructed and sequenced small RNA libraries from sporophytes and gametophytes of P.haitanensis.Five micro RNAs were identified that shared no sequence homology with known micro RNAs.Our results indicated that P.haitanensis might posses a complex s RNA processing system in which the novel micro RNAs act as important regulators of the development of different generations of P.haitanensis.     

STUDIES ON COLOR TYPE VARIANTSFROM MUTAGENIZED PROTOPLASTS OFPORPHYRA HAITANENSIS CHANG ET ZHENG& P. YEZOENSIS UEDA (RHODOPHYCEASE )

Isolated protoplasts from thalli of Porphyra haitanensis and Porphyra yezoensis were treated with colchicine or irradiated by ultraviolet (UV ). Several types of color variants were observed among the protoplast offspring. After treatment with colchicine: (1) 0.04-0.09% of red type variants in P. haitanensis were obtained; (2) The rate of red type variants and the variegated chimeral thalli composed of red type and wild type of sectors were 6.31- 1.11% in P. yezoensis. After irradiation with UV: (1) 3.5- 10.5% of red type variants in P. yezoensis were obtained: (2) 0.5-2-0% of red type variants and the variegated chimeral thalli composed of red type and wild type of sectors were obtained in P. haitanensis. Colchicine and UV's mutangenic effects on P. yezoensis protoplasts were stronger than those on P. haitanensis protoplasts. The most efficient concentration of colchicine was 0.05%. The optimal length of UV-radiation was 1/2 min (radiation distance 5 cm). The red type variants induced, by colchicine tr     

COMPARATIVE STUDIES ON STRUCTURAL FEATURE OF AGAR POLYSACCHARIDES FROM PORPHYRA HAITANENSIS GROWN IN SOUTH AND NORTH CHINA

The structural feature of agar polysaccharides from Porphyra haitanensis grown in south China and transplanted to the north was investigated by fractioriation on DEAE - Sephadex A 50, chemical analysis, and infrared and 13C - NMR spectroscopy.The agars composed mainly of Charged molecules were eluted from DEAE - Sephadex A 50 with 1.0 mol/L- NaCl solution from the southern P. haitanensis and with 0.5 mol/L NaCl from the northern one. The 13C-NMR spectra showed that agarobiose [(1 -3)- B D - galactopyranosyl - (1 -4)-3,6-anhydro-a-L-galactopyranose] and the biological precursor of agarobiose [(1-3} B-D-galactopyra nosyl- (1-4)- 6- sulfate-a- L-galactopyranose ] were the major disaccharide repeating units in the charged fractions. The content of the biological precur sor in the agar polysaccharides from southern P. haitanensis was higher than that in the northern one, the content of the biological precursor extracted from cold water was higher than that from hot water, and the content of 6- OMe-D-galactos     

On microbial community of Pyropia haitanensis by metagenomic analysis

Microorganisms plays an important role in the growth of Pyropia haitanensis. To understand the structural and functional diversity of the microorganism community of P. haitanensis(PH40), the associated metabolic pathway network in cluster of orthologous groups(COG) and Kyoto Encyclopedia of Genes and Genomes(KEGG), and carbohydrate-active enzymes(CAZymes) were explored in metagenomic analysis. DNA extraction from gametophytes of P. haitanensis was performed first, followed by library construction, sequencing, preprocessing of sequencing data, taxonomy assignment, gene prediction, and functional annotation. The results show that the predominant microorganisms of P. haitanensis were bacteria(98.98%), and the phylum with the highest abundance was Proteobacteria(54.64%), followed by Bacteroidetes(37.92%). Erythrobacter(3.98%) and Hyunsoonleella jejuensis(1.56%) were the genera and species with the highest abundance of bacteria, respectively. The COG annotation demonstrated that genes associated with microbial metabolism was the predominant category. The results of metabolic pathway annotation show that the ABC transport system and two-component system were the main pathways in the microbial community. Plant growth hormone biosynthesis pathway and multi-vitamin biosynthesis functional units(modules) were the other important pathways. The CAZyme annotation revealed that the starch might be an important carbon source for microorganisms. Glycosyl transferase family 2(GT2) and glycosyl transferase family 3(GT3) were the highly abundant families in glucoside transferase superfamily. Six metagenome-assembled genomes containing enzymes involved in the biosynthesis of cobalamin(vitamin B 12) and indole-3-acetic acid were obtained by binning method. They were confirmed to belong to Rhodobacterales and Rhizobiales, respectively. Our findings provide comprehensive insights into the microorganism community of Pyropia.     

An improved method for karyotype analyses of marine algae

Modified carbol fuchsin staining method was successfully introduced into the karyotype analyses of marine algae, in-cluding Porphyra, Undaria pinnatifida and Laminaria japonica. Haploid chromosomes were numbered clearly in the vegetative, spermatangial and conchosporangial cells of P. haitanensis and P. yezoensis. Diploid chromosomes were observed and numbered in immature conchosporangial cells of P. haitanensis and P. yezoensis. Pit-connections of Porphyra were also clearly demonstrated. Prophase chromosomes of conchocelis cells were also clearly stained with modified carbol fuchsin. One molar per liter hydrochloric hydrolysis at 60℃ for 7-8min is necessary for getting transparent cytoplasm for conchosporangial karyotype analysis of Porphyra. Staining effects of the three methods using iron alum acetocarmine, aceto-iron-haematoxylin-chloral hydrate and modified carbol fuchsin were compared on the vegetative, sperrnatangial and conchosporangial cells of Porphyra and the gametophytes of U. pinnati-fida and L. japonica. Among the three methods, the modified carbol fuchsin method gave the best result of deep staining and good contrast between nucleus and cytoplasm.     

Pyropia haitanensis polysaccharide extends lifespan by inhibiting protein aggregation in Caenorhabditis elegans

Pyropia haitanensis poly saccharide(LP) have been found for having many excellent functions such as anti-aging.Using Caenorhabditis elegans models,we evaluated the anti-aging activity of LP by observing the lifespan,reproduction,pharyngeal pumping,stress response,quantitative fluorescence of poly glutamic acid,and nuclear localization of DAP-16 of worms.The results reveal that LP could extend the adult lifespan of wild-type and polyQ nematodes,indicating a connection of its anti-aging benefit with the toxicity-suppressing effect The number of polyglutamic acid aggregates in high concentration groups decreased by 24.39%(P0.05) to the control.The high-dose group strongly induced DAP-16 nuclear translocation over intermediate and cytosolic localizations compared with the control(P0.001).Therefore,we believe that LP could extend the lifespan and reduce the protein aggregation in C.elegans through nuclear DAF-16::GFP expression.     

Identification and characterization of a Dna J gene from red alga Pyropia yezoensis(Bangiales,Rhodophyta)

Members of the Dna J family are proteins that play a pivotal role in various cellular processes, such as protein folding, protein transport and cellular responses to stress. In the present study, we identified and characterized the full-length Dna J c DNA sequence from expressed sequence tags of Pyropia yezoensis( Py Dna J) via rapid identification of c DNA ends. This c DNA encoded a protein of 429 amino acids, which shared high sequence similarity with other identified Dna J proteins, such as a heat shock protein 40/Dna J from Pyropia haitanensis. The relative m RNA expression level of Py Dna J was investigated using real-time PCR to determine its specific expression during the algal life cycle and during desiccation. The relative m RNA expression level in sporophytes was higher than that in gametophytes and significantly increased during the whole desiccation process. These results indicate that Py Dna J is an authentic member of the Dna J family in plants and red algae and might play a pivotal role in mitigating damage to P. yezoensis during desiccation.     

Sequence analysis of the ITS region and 5.8S rDNA of <Emphasis Type="Italic">Porphyra haitanensis</Emphasis>

The sequences of the ITS(internal transcribed spacer) and 5.8S rDNA of three cultivated strains of Porphyra haitanensis thalli(NB,PT and ST) were amplified,sequenced and analyzed.In addition,the phylogenic relationships of the sequences identified in this study with those of other Porphyra retrieved from GenBank were evaluated.The results are as follows:the sequences of the ITS and 5.8S rDNA were essentially identical among the three strains.The sequences of ITS1 were 331 bp to 334 bp,while those of the 5.8...     

The UV-absorbing compounds in gametophytic blades of Porphyra haitanensis and the effect on formation of sporangial branchlets of conchocelis

Endogenous levels of UVAC in different parts of matured gametophytic blades of Porphyra haitanensis and the effect of exogenous UVAC on sporangial branchlet formation are studied. The UVAC value in vegetative (36. 78 mg/g) and reproductive (29.25 mg/g) parts of female thallus is higher than their counterparts in male thallus (34.68 mg/g and 20.46 mg/g). The UVAC value of reproductive parts is higher than vegetative parts of same sexual thallus. The optimal UVAC concentration for sporangial branchlet formation is 0.1 mg/g.     

Studies on intercropping ofPorphyra haitanensis T. J. Chang et B. F. Zheng with Bay ScallopArgopecten irradians Lamarck

Results of the experiment of northward transplantation ofPorphyra haitanensis showed that intercrop ofP. haitanensis with Bay Scallop is feasible because the growth duration of both of them is about the same in August–December. Full use of cultivating area, lowering its cost and increasing its product per unit area as well as improving the ecological environment are realized during intercrop. Contribution No. 2152 from the Institute of Oceanology, Academia Sinica.     

An improved PCR method for direct identification of Porphyra （Bangiales, Rhodophyta） using conchocelis based on a RUBISCO intergenic spacer

An improved method of PCR in which the small segment of conchocelis is amplified directly without DNA extraction was used to amplify a RUBISCO intergenic spacer DNA fragment from nine species of red algal genus Porphyra(Bangiales,Rhodophyta),including Porphyra yezoensis(Jiangsu,China),P.haitanensis(Fujian,China),P.oligospermatangia(Qingdao,China),P.katadai(Qingdao,China),P.tenera(Qingdao,China),P.suborboculata(Fujian,China),P.pseudolinearis(Kogendo,Korea),P.linearis(Devon,England),and P.fallax(Seattle,USA)....     

Morphology and phylogeny of Chrysogorgia pinniformis sp.nov.and C.varians sp.nov.,two golden corals from the Caroline seamounts in the tropical Western Pacific Ocean

Two new species of Chrysogorgia Duchassaing Michelotti,1864 collected from the Caroline seamounts in the tropical Western Pacific Ocean are described.Chrysogorgia pinniformis sp.nov.belongs to Versluys' group C(Squamosae typicae) with only scales in polyp body wall and tentacles.C.pinniformis sp.nov.is characterized by large branches pinnately branched and forming multiple fans with its small branches regularly and quasi-dichotomous branched,and scales and rods present in the polyp mouth area.It is most similar to C.pinnata Cairns,2007 by the pinnate trait,but differs from the latter by its group C pattern(vs.group A,Spiculosae) and having sclerites present in the polyp mouth area(vs.absent).Chrysogorgia varians sp.nov.belongs to the Chrysogorgia group A(Spiculosae) with rods distributed in the polyp body wall and tentacles.It is characterized by warty rods and elongated scales in the tentacles,many warts and ridges on the scales,conspicuously toothed margins at the rounded ends in the pinnules,and small rods with ridge-like warts in the polyp mouth area.This species was frequent and abundant in the Caroline seamounts during our cruises and its morphological variability in growth period was obvious.The phylogenetic analyses based on mtMutS and 28 S rDNA regions supported the assignment of the new species to the genus Chrysogorgia.However,the mtMutS marker showed very limited usefulness for species delimitation and inner relationship inference of Chrysogorgia.In contrast,the 28 S rDNA showed much higher level of genetic variation,and it may be a potential barcode for this genus.In the 28 S rDNA trees,the two new species clustered together.Additionally,compilation of our data showed that 42 of 78(ca.54%) Chrysogorgia species were found in the Indo-West Pacific convergent region,indicating that this area may be a hotspot of deep-water Chrysogorgia species.     

Identification and bioinformatics analysis of microRNAs from the sporophyte and gametophyte of <Emphasis Type="Italic">Pyropia haitanensis</Emphasis>

Pyropia haitanensis (T. J. Chang et B. F. Zheng) N. Kikuchi et M. Miyata (Porphyra haitanensis) is an economically important genus that is cultured widely in China. P. haitanensis is cultured on a larger scale than Pyropia yezoensis, making up an important part of the total production of cultivated Pyropia in China. However, the majority of molecular mechanisms underlying the physiological processes of P. haitanensis remain unknown. P. haitanensis could utilize inorganic carbon and the sporophytes of P. haitanensis might possess a PCK-type C₄-like carbon-fixation pathway. To identify microRNAs and their probable roles in sporophyte and gametophyte development, we constructed and sequenced small RNA libraries from sporophytes and gametophytes of P. haitanensis. Five microRNAs were identified that shared no sequence homology with known microRNAs. Our results indicated that P. haitanensis might posses a complex sRNA processing system in which the novel microRNAs act as important regulators of the development of different generations of P. haitanensis.     

Assessing adaptability of planted trees using leaf traits: A case study with Robinia pseudoacacia L. in the Loess Plateau, China

Leaf trait patterns and their variations with climate are interpreted as an adaptive adjustment to environment.This study assessed the adaptability of planted black locust (Robinia pseudoacacia L.) based on the analysis of leaf traits and the comparison of its leaf traits with inter-specific ones existing in the same area.We measured some water and N use related leaf traits: leaf dry mass per unit area (LMA) and N,P and K concentrations based on both leaf area (Narea,Parea and Karea) and leaf mass (Nmass,Pm...     

滨州—高青地区深层淡水供水水文地质条件初步评价

滨州-高青地区位于山东省东北部,东临博兴县,西与济阳县相接。北部为黄河冲洪积平原,南部为鲁中山区山前倾斜平原。黄河穿越该区北部,小清河流经南部,主要地表水和主要供水水源为黄河水。由于大部分地区缺乏浅层地下淡水,在引黄灌溉所达不到之地区,特别是南部,主要供水水源仍需开发利用深层地下水。该文对滨州-高青地区的地质构造特征进行了论述,对滨州-高青地区深层淡水供水水文地质条件初步评价,为该区的工农业和油田的勘探开发建设的合理用水,提供一定理论依据。     

陕西洛南洛源原生金地球化学异常特征与洛河砂金矿预测

洛源原生金地球化学异常具有浓集中心分散、规模小、几何浓度内带大面积出露、组分较复杂和水平分带不清等特征,表明洛源金矿化主要为分散矿化,而且具较大的剥蚀深度。金的原生地球化学异常源为小的含金断裂构造带。地球化学异常区的冰川活动,加速了此区的剥蚀作用。洛河砂金矿是在上淤汇水盆地中金的分散矿化被强烈剥蚀下形成的。金的分散矿化范围大,空白水系少,稀释作用受仰制,为洛河砂金矿形成创造了良好的条件。从洛河的淘金历史和现状以及对砂金的总量预测来看,洛河砂金矿是很有远景的。     

基于SB-DInSAR时间序列探测地震形变过程的研究

对2009年4月6日意大利拉奎拉6.3级地震9景ENVISAT重轨单视复数据采用双轨模式进行小基线（SB）干涉处理,获得了此次地震的SB-DInSAR时间序列干涉图、位移场以及位移速度场。结果表明：1)地震位移场形变过程为由震前的缓慢变化到震后的加速运动,速度明显由慢到快;2）震中以西为大面积隆起区,最大隆起量为140 mm;震中以东为大面积下沉区域,但形变集中在破裂区,主要在发震过程及震后形成,最大沉降量达185 mm;3）地震产生的破裂主要沿震中的东南方向传播,集中在约13.4 km×7.6 km、方向约135°的椭圆形区域内。     

THE WET-DRY CHANGES IN RECENT 40YEARS IN TAKLIMAKAN AREA

IINTRODUCTIONAlongwiththePetroleumexploitationofTarimBasin,consideringaboutwaterresources,peoplepaidmuchattentiontothestudyofthemodernclimatechangeinTaklimakanarea.SomePeoplehaddonemanalySeSwiththemeteorologicaldatainthearea(Yang,1992;Jietal.,1992;Zheng,1991;Xue,1989;Li,1989;Wenetal.,1988).BasedontheO~ionaldstaofprecipitationinrecent40years,thisPaperCarefullyanalyZedthewet-drychangeinrecentseveraldeCadesinTaklilnakanareaanditsneighdringareas(thenorthernXinjiang,QinghaiandGansuPro…     

基于D—InSAR的济宁市采煤沉陷监测与分析

对地下采煤造成的地表沉陷进行及时、精确的监测监管是一个亟待解决的课题。该文以济宁市为例,介绍了D-InSAR技术在大范围采煤沉陷区监测及其时序变化特征分析中的应用。介绍了使用D-InSAR技术对采煤沉陷进行监测的基本原理;以5cm下沉线作为采煤沉陷区边界线,选取15期RADARSAT-2 Wide模式数据,采用多基线累积叠加方法分别提取各期采煤沉陷区边界并使用岩移观测数据对监测结果进行了检核。以花园煤矿为例,分析D-InSAR监测采煤沉陷范围及程度的时序变化特征,随着开采的持续进行,沉陷范围及沉陷量逐步增加,受临近工作面开采影响,沉陷中心的沉陷量呈周期性增加。结果表明,D-InSAR可以精确有效地对采煤沉陷区范围及其时序变形过程进行监测,可以应用于大尺度采煤沉陷区的动态监测。     

山东省区域地球物理场

山东省各岩石磁性及密度参数基本可分为高、中、低3类.山东省磁性结晶基底为泰山岩群、荆山群、胶东岩群、胶南岩群.以沂沭断裂带为界,山东省区域地球物理场分为鲁东区和鲁西区.鲁东区进一步划分为3个次级区,即北部区、中部区和南部区.鲁西区进一步划分为5个次级区,即鲁北重磁缓变区、鲁西南重高磁高区、中部弧形重磁场区、鲁西中部重低磁高区、鲁中南条带状重磁场区.各区地球物理场由于地质原因各具特点.山东省莫氏面总体呈东浅西深的缓变带,在该缓变带的背景上,呈周边地区条带状幔隆、中部幔坳的特征.山东省居里面呈近南北向带状分布,有德州聊城菏泽隆起带、滨州淄博滕州深坳带、蓬莱海阳千里岩响水隆起带.鲁中隆起北部碰撞带为山东省特殊的构造地带,其特征为侵入岩体沿该带断续出现、岩体围岩或岩体内铜及多金属矿化较为普遍、地热田沿该带时有分布、地形上为南部山区和北部平原的分界地带.胶莱盆地内,蓼兰-高密缺失莱阳群层位;中生代早期,盆地东北部与西南部是盆地的沉积中心,至晚期盆地中心北移,第三纪沉积中心则为平度蓼兰附近;盆地较深部位位于诸城凹陷、平度凹陷.胶莱盆地的次级构造单元可划分为11个,七级镇构造带控制了胶莱盆地的成生和发展.山东省岩浆岩通常表现为强磁性、低密度,但基性程度高的岩体磁性高、密度亦高,酸性程度高的岩体磁性低、密度低.