Selective grazing by cattle on spatially and seasonally heterogeneous rangeland in Sahel

The selective use by cattle of range resources in the Sahel was assessed in terms of quantity and nutritional quality of the herbage grazed and diet selected. Peak standing crop was 1951 kg ha⁻¹dry matter (DM) with 47·9 kg nitrogen (N) ha⁻¹and 7·4 kg phosphorus (P) ha⁻¹. Esophageally fistulated cattle diets were consistently (p<0·05) higher in N, P and organic matter digestibility (OMD) than the herbage grazed. Cattle consumed 48% of the mean annual herbage production. The results indicate grazing ruminants select a higher quality diet from Sahelian rangelands than can be predicted on the basis of pasture evaluation alone. Therefore, the foraging behaviour of the animals needs to be considered in the evaluation of Sahelian rangelands for animal production.     

Tiller recruitment and mortality in the dryland bunchgrass Eragrostis curvula as affected by defoliation intensity

A greenhouse experiment was conducted to investigate the effects of moderate (14 cm) vs. severe (7 cm) defoliation on tiller recruitment and mortality within the interior and on the perimeter of weeping lovegrass (Eragrostis curvula) tussocks. Tiller number per marked shoot within the interior was significantly greater in plants defoliated at 14-cm stubble height than at 7-cm stubble height (2·37 vs. 0·43, p<0·05) 28 weeks after defoliation; likewise, tiller number per marked shoot was also greater on the perimeter of tussocks in the higher stubble than the lower stubble defoliation treatment (5·10 vs. 2·03, p<0·05). Tiller natality increased significantly 4 weeks after defoliation except for tillers cut to 7-cm stubble height within the interior of tussocks where tiller natality reached a peak at 4·5 weeks after defoliation. During the later stages of canopy development, tiller numbers per marked shoot gradually declined as tiller mortality increased. Tillers began senescing 13 weeks after defoliation, and the senescence rate was 52% greater in the 7-cm stubble height treatment than in the 14-cm stubble height treatment (2·33 vs. 1·53, p<0·0001). Within the interior of tussocks, number of the marked tillers more than doubled under the 14-cm stubble height treatment, whereas the plants lost 57% of the monitored tillers under the 7-cm stubble height treatment. We concluded that the central dieback process in weeping lovegrass is accelerated by severe defoliation.     

Saxicolous and riparian vegetation of a piedmont in central-western Argentina

Four plant communities were determined through floristical and non-metric analysis: a Dolichlasium lagascae community in crevices on sunny rock outcrops; a Cercidium praecox ssp. glaucum community on sunny slopes; an Artemisia mendozana–Adesmia trijuga community on shady slopes; and a Eupatorium buniifolium community on dry riverbanks. The discriminant function analysis showed that the first group corresponds to the sunny rock outcrops discriminated by Na content (r=0·53, p<0·05), the second group is for sunny slopes discriminated by electric conductivity and the third for shady slopes–riparian discriminated by the (Ca+Mg) content (r=0·54, p<0·05). A pattern of primary succession on outcrop surfaces is proposed.     

Effects of indole-3-acetic acid and zinc on the growth, osmotic potential and soluble carbon and nitrogen components of soybean plants growing under water deficit

Three-week old soybean (Glycine max) plants were subjected to a factorial combination of four regimes of soil matric water potential (ψ_m=−0·03, −0·5, −1·0 and −1·5 MPa), two levels of supplementary Zn (O and 20 mgl⁻¹) and two levels of foliar IAA application (O and 10 mgl⁻¹). Under control conditions (no Zn, no IAA), increasing soil drying progressively retarded shoot and root growth (length and dry mass production), reduced leaf relative water content (RWC) and decreased the contents of chlorophyll (Chl) and shoot soluble sugars (SS), but increased soluble sugar content of roots and lowered osmotic water potential of shoots and roots (osmotic adjustment). Total free amino acid (TAA) content increased in shoots but decreased in roots whereas contents of soluble proteins (SP) decreased in shoots and roots. The effect of water stress was statistically significant (p<0·05) and had a major effect (as indicated by η²values) on leaf RWC, shoot and root dry masses and osmotic potential. Supplementary Zn improved root growth at all levels of stress and shoot growth under severe stress. Improvement of growth was positively correlated with the internal tissue Zn concentrations (r=0·91 and 0·86 for shoot and 0·94 and 0·82 for root length and dry mass respectively). Exogenous IAA raised (p<0·05) RWC, Chl, DM (slightly), root SS, and SP, whereas shoot TAA was lowered. Effects on root TAA and shoot SS were more complex: they were lowered at zero stress and raised under severe stress. IAA and Zn in combination had additive effects on Chl, growth and osmotic potential, but their combined effects on SP and TAA were more complex. It is concluded that the treatment of soybean plants grown under conditions of low soil water potentials and Zn deficiency with Zn and IAA solutions counteracted the deleterious effects of stress, especially at high stress levels, and helped stressed plants to grow successfully under these adverse unfavourable conditions.     

Effect of nitrogen amendments on microbial biomass, above-ground biomass and nematode population in the Negev Desert soil

Nitrogen amendments (0, 25, 50 and 100 kg NH₄NO₃/ha) were used to study the responses of primary production, microbial biomass and nematode population in desert soil. The study was conducted in the Israeli Negev Desert, a region characterized by low and randomly distributed rainfall. Over a 1-year study period, nitrogen amendments resulted in a significant (p<0·01) increase in soil microbial biomass. Soil microbial biomass also increased concomitantly with the increase in soil organic matter. The number of free-living nematodes in the soil increased with the increase in soil moisture, ranging from 43,000 individuals per square metre at the end of the summer to 351,000 individuals per square metre during the rainy season. No significant correlation was found between the nitrogen treatments and the nematode population, whereas a significant positive correlation was found between the nitrogen amendments and the above-ground biomass (r²=0·94, p<0·03). The nitrate proportion of the total soluble nitrogen in the soil also increased with the increase in soil moisture. This study provides baseline data for nitrogen amendments on soil microbial status, as well as insights into the importance of nitrogen in fertility in arid environments.     

Extractable and total Fe and Mn contents of three sand dune soils in NW Nigeria

The profile distribution of total, diethylene triamine penta-acetic acid (DTPA)- and 0·1 HCl-extractable Fe and Mn were determined in 12 pedons formed on three contiguous sand dunes in the semi-arid savanna of Nigeria. The total Fe and Mn contents varied from 100 to 3750 and 40 to 11,375 μg g⁻¹, respectively. Values of 0·1 HCl-extractable Fe and Mn varied from 8·0 to 123 and 1·5 to 43·5 μg g⁻¹, respectively. The corresponding values of DTPA-extractable Fe and Mn were 4·5 to 16·0 and 1·0 to 38·8 μg g⁻¹. Total Fe and Mn correlated significantly with clay in nearly all the dunes (p≤ 0·01) but were not significantly correlated with organic matter. The 0·1 HCl-extractable Mn had a positive significant correlation with soil pH (r= 0·58*) in Illela dunes but a negative significant correlation with pH in the Sangiwa dunes (r= −0·75***). The values of extractable Fe and Mn in the sand dune soils are above the critical limits reported in other literature, thus deficiencies of these micronutrients do not pose a problem to crop production in the study area.     

The application of a remotely-sensed diversity index to monitor degradation patterns in a semi-arid, heterogeneous, South African landscape

We present a remotely-sensed degradation assessment technique based on the measurement of landscape heterogeneity (a key determinant of degradation status). The moving standard deviation index (MSDI) is derived by passing a moving standard deviation filter across the Landsat TM band 3 (red band). Degraded/unstable landscapes exhibit higher MSDI values than their undisturbed/stable counterparts. Significant differences in MSDI were detected (p<0·001) across four fence-lines which separated rangeland of contrasting condition. The relationship of the index to normalized difference vegetation index (NDVI) was tested in five different ecosystems and significant correlations were obtained in all cases (p<0·001). We propose the MSDI as a powerful adjunct to vegetation indices.     

Relationship between species richness of small mammals and primary productivity of arid and semi-arid grasslands in north China

We studied the relationship between primary productivity and species richness of small mammals at both large and small spatial scales in the arid and semi-arid grasslands of north China. The productivity (x)–species richness (y) pattern at a large spatial scale can be described by a unimodal quadratic regression curve (y=7·41+0·1^*x−0·0003^*x²,p =0·008, r²=0·788). At a small spatial scale, however, neither linear nor quadratic regression fit the data for 1980 and 1994 (p>0·25). Primary productivity may not be an appropriate predictor of the species richness of small mammals at a small spatial scale. We conclude, therefore, that the primary productivity–species richness pattern of small mammals may be scale-dependent in the arid and semi-arid grasslands of north China. Landscape complexity should be considered in future studies of productivity–richness relationships.     

Relation between semen quality and rangeland diets of mixed-breed male goats

The impact of range plant toxins on the reproductive performance of female cattle is economically important to the livestock industry. Nevertheless, there is little information on the significance of toxicosis from range plants on semen quality in domestic ruminants. A total of 18 adult mixed-breed bucks under range conditions were used to evaluate the effect of diet composition (microhistological analysis of fecal samples) on some semen characteristics, and blood and fecal components in April and August. Forages in the buck diets were grouped into 2 classes: low or high levels of each plant in the buck diets. Bucks with higher proportion of Acacia greggii, Flourensia cernua and Lindleya mespiloides in their diets yielded 23–50% less (p<0.05) semen than bucks with low proportion of these shrubs in their diet. Sperm motility was reduced by 3–8 percent units (p<0.05) by high levels of five rangeland shrubs in the buck diets. Bucks consuming high levels of Acacia greggii showed significantly lower percentages of normal sperm (92±10 vs. 96±3; p<0.05) than bucks with low intake of this forage. High proportions of Rhus virens and Solanum elaeagnifolium in the buck diet increased the percentage of normal sperm (5 units, p<0.05) when compared to bucks with low utilization of these forages. Increased intake of Acacia greggii, Dalea bicolor, Opuntia rastrera, Larrea tridentata and Cowania plicata reduced percentage of live sperm by 5 units (p<0.05). Blood metabolites analysis showed that increased levels of Acacia greggii and Parthenium incanum were related to better nutritional status of bucks. Zn (−), Cu (−) and glucose (+) were significant predictors of semen volume (r²=0.30). Cu (−) and cholesterol (−) accounted for 37% of the variation in percentage of normal sperms. Forty-four percent of the variation in secondary abnormalities was accounted by Zn (+) and cholesterol (+), whereas fecal N, serum total proteins and creatinine positively affected percentage of live sperms (r²=0.37). These results indicate that semen quality and metabolic profiles of grazing bucks were sensitive to the ingestion of some Chihuahuan desert forages.     

Influence of understory removal, thinning and P fertilization on N2fixation in a mature mesquite (Prosopis glandulosavar.glandulosa) stand

The natural abundance¹⁵N/¹⁴N method was used to estimate the influence of silvicultural and P fertilization treatments on N accretion, N₂fixation and N partitioning among tissues in a mature mesquiteProsopis glandulosavar.glandulosastand in Texas. The silvicultural treatments consisted of understory removal, herbicide treatment of brushy resprouts, thinning trees to single stems and 100 kg ha⁻¹P fertilization. The trees had a mean basal diameter of 17·8 cm with 8 to 35 cm range. The stand was slow growing with the increase in dry matter ranging from 0·465 Mg ha⁻¹year⁻¹to 0·701 Mg ha⁻¹year⁻¹for the 8 years after the treatments were applied. N accretion after 8 years ranged from 3·1 kg ha⁻¹year⁻¹to 4·4 kg ha⁻¹year⁻¹.Due to the range in δ¹⁵N of the leaves, twigs, branches and trunk, we used the weighted (by biomass) average δ¹⁵N per tree in calculations of the percent N derived from N₂fixation (%Ndfa). There was considerable variability in δ¹⁵N of the reference plants, i.e. from 3·3 to 5·9. In contrast there was low variability in the background δ¹⁵N of nearby soils (7·0±1·0). As the total above-ground biomass δ¹⁵N of a grass grown outside the influence of mesquite (7·8±0·58) had the same δ¹⁵N as the soil (7·5±1·0), we used the grass outside the influence of mesquite and the weighted tree mean δ¹⁵N to calculate % of N derived from N₂fixation.The decrease in intraspecific competition by thinning multistemed trees to single stemmed trees was the only treatment that significantly (p= 0·0001) increased growth. Interspecific competition, i.e. understory removal, did not increase growth. There were no significant differences in total N production or N fixation among treatment means. The most striking result was the highly positive correlation between tree δ¹⁵N and total N per tree and biomass per tree (R²= 0·90,F= 164·4, df. = 18, mean square error (MSE) = 0·155,p= 0·0001). This implies that the younger trees colonizing infertile soils relied more heavily on N₂fixation than larger trees which accumulated 1200 kg ha⁻¹more N under their canopies. The percentage N derived from N₂fixation ranged from 63 to 73% in the various treatments. Despite the high percentage of N derived from N₂fixation, the N₂fixation of the stand was very low, i.e. 1·98 to 2·80 kg N ha⁻¹year⁻¹, due to the low growth of the stand. We believe that comparisons of the whole tree weighted δ¹⁵N to background soil δ¹⁵N provides a more reasonable approach to estimate % N₂fixation than comparisons of leaves of fixers and reference plants.     

Smith Canyon dune field, Washington, U.S.A: relation to glacial outburst floods, the Mazama eruption, and Holocene paleoclimate

Sedimentary deposits from the Smith Canyon dune field, south-central Columbia Basin, Washington, U.S.A. document climatically-influenced Late Pleistocene and Holocene aeolian and fluvial deposition in a region impacted by glacial outburst floods and tephra falls. The depositional history is summarized by five environmentally distinctive and climatically sensitive sedimentary units (temporal limits estimated): Unit 1 (c. 15·5–8 ka), pedogenically altered glacial outburst flood and minor aeolian silt and clay; Unit 2 (c. 8–6·9 ka), fluvial and minor aeolian sand; Unit 3 (c. 6·9–6·8 ka), flood-induced fluvial sand with gravel-sized tephra clasts; Unit 4 (c. 6·8–3·9 ka), aeolian dune sand; Unit 5 (c. 3·9 ka to present), pedogenically altered, stabilized dune sand. Estimated age ranges are based on stratigraphic position, tephrochronology, and correlation with temporally constrained strata from elsewhere in the region.     

Effects of body mass and temperature on standard metabolic rate of the herbivorous desert lizardUromastyx philbyi

The standard metabolic rate ofUromastyx philbyiwas measured at 20–40 °C, using constant pressure manometric respirometers. Standard metabolic rate was mass-dependent. Values for the mass exponent ‘b’ ranged from 0·75 at 20 °C to 0·82 at 40 °C. Standard metabolic rate increased as temperature increased with high Q₁₀values at low temperatures (20–25 °C). Low standard metabolic rates in this species are probably an adaptation to herbivory in hot desert environments.     

The comparative influence of past management and rainfall on range herbaceous standing crop in east-central Argentina: 14 years of observations

Impacts of six initial treatments and rainfall on maximum above-ground herbaceous standing crop were assessed in 1978 and annually from 1984 to 1992 in rangelands of east-central semi-arid Argentina. Treatments applied in 1977–1978 were: (1) untreated control; (2) burning; (3) herbicide application (shrub control); (4) Old Field 1 or (5) Old Field 2 (areas previously exposed to different degrees of mechanical soil disturbance for 25 years); and (6) overgrazing. Domestic herbivores were excluded thereafter from all treatments until 1993.During 1984–1992, total above-ground herbaceous standing crop often remained greater in both Old Fields than in the other treatments; highest mean values were 2336 and 1640 kg ha⁻¹for Old Field 1 and Old Field 2 treatments, respectively. Lowest total above-ground standing crops in all treatments (M = 296–475 kg ha⁻¹) occurred in 1989, the year with the lowest annual rainfall (257·5 mm). Desirable perennial grasses contributed most (44–100%) to total herbaceous standing crop in all treatments during the study period. Most of this standing crop was made up of the cool-season grassesPiptochaetium napostaense, Poa ligularis, Stipa clarazii, S. papposaandS. tenuis. Annual rainfall was closely related (p< 0·05) to total herbaceous and desirable perennial grass standing crops in most treatments, and accounted for most of the variation in herbage production between years.     

Plant life-form and germination in a Mexican inter-tropical desert: effects of soil water potential and temperature

We investigated the effects of soil water potential (SWP) and temperature on seed germination of six coexisting species of an inter-tropical desert. These species include three life-forms; the shrubs Cercidium praecox andProsopis laevigata ; the columnar succulents Neobuxbaumia tetetzo and Pachycereus hollianus; and the arborescent semi-succulents Beaucarnea gracilis and Yucca periculosa. In the six species germinability increased and germination time and speed of germination (t⁵⁰) decreased as SWP decreased. The SWP treatments were 0 MPa, −0·12 MPa, −0·2 MPa, −0·41 MPa and −0·66 MPa. There was, however, a SWP threshold below which germination time and t⁵⁰increased and germinability decreased. The shrubs had the highest germinability whereas the columnar succulents had the lowest. The shrubs also had shorter germination time andt⁵⁰ than arborescent semi-succulents whereas seeds of the columnar succulents were indeterminate. In all species except P. laevigata germinability increased and the germination time and t⁵⁰decreased as temperature increased. The temperature treatments were 12°C, 20°C and 26°C. The shrubs had the shortest t⁵⁰and germination time and the highest germinability at all temperatures. Arborescent semi-succulents had the lowest germinability and longest germination time and t⁵⁰at the three temperatures treatments. Our results support the hypothesis that in desert environments different plant life-forms utilise different germination strategies to persist.     

Biodegradation of municipal refuse and chicken manure in a winter-wheat ecosystem in Saudi Arabia

Addition and incorporation of organic fertilizers is known to improve the properties of agricultural soils. Therefore, during 22 weeks of field incubation we studied the biodegradation (measured by static absorption of CO₂) of two organic manures viz. municipal refuse and chicken manure incorporated into sandy soils, under sprinker irrigation, at the rates of 0, 16·5, 33·0, 49·5 and 66·0, and 0, 4·1, 8·25, 16·5, and 33·0 tonnes/ha, respectively.The results obtained indicated that the cumulative amount of CO₂ respired was linearly correlated with the rate of organic material applied (p < 0.001). Statistical analysis of the data obtained showed that both the intercept (rate of decomposition) and the slope (rate of reaction with time) increased with increasing rate of application of organic manure. The magnitude of increment was highest at the application rates of 33·0 and 8·25 tonnes/ha for municipal refuse and chicken manure, respectively. The rate of increment, generally, declined thereafter thus indicating that these two rates are the optimum levels of incorporation of the appropriate organic manures under the conditions of this study.The maximum organic-carbon mineralized, accounting for 10·5 and approximately 50%, was obtained at the optimum rates for municipal refuse and chicken manure, respectively. Both simple and multiple regression equations describing the relationship between soil temperature and soil-moisture content each separately and in combination, as independent variables, and the cumulative rate of CO₂ production were computed. A substantially strong relationship with soil temperature but inconsistent and contradicting results with soil moisture were obtained. Inclusion of soil temperature and moisture in a single model as an independent variable in relation to cumulative CO₂ as a dependent variable improved the soil moisture-CO₂ production relationship.     

Leaching requirement and salinity threshold for the yield and agronomic characteristics of halophytes under salt stress

An experiment was carried out at Nahshala Farm, north west of Al-Ain City, U.A.E. during the 1998–2000 growing seasons, using six halophytes (Batis maritima, Distichlis spicata, Juncus roemerianus, Paspalum vaginatum, Salicornia bigelovii and Spartina alterniflora) and two levels of leaching fraction (0·25 and 0·50) under three irrigation salinity levels (10, 20, and 40 gL⁻¹) in a randomized complete block design arranged in split plots. The purpose of the research was to apply the theory of crop salt tolerance on aboveground yields and agronomic characteristics of halophytes to determine their thresholds for salinity and to determine leaching requirements. The results indicated that the halophyte species tested can grow with minimum reduction in the growth potential at <20 gL⁻¹of mean salinity of soil solution. Leaching fraction (LF) of 0·25 at the highest salinity of irrigation water (40 gL⁻¹) was inadequate to attain the steady-state salt balance during the growth period, although the drainage salinity reached more than 90 g L⁻¹. Furthermore, if the same level of LF is used for longer period, soil salinity under this high salt treatment will continue to rise, and plant growth may deteriorate. Leaching fraction of 0·50 is preferable if salinity of irrigation water was more than 20 gL⁻¹and dry matter production is considered, although the amount of water use will be excessive     

Micropropagation of Acacia seyal Del. in vitro

In vitro mass propagation of Acacia seyal Del. was achieved using seedling shoot tip explants on Murashige and Skoog (MS) medium supplemented with 6-benzyladenine (BA) or Thidiazuron (TDZ) with ∝-Naphtalene acetic acid (NAA). The best result was obtained with BA in the presence of NAA. The greatest shoot multiplication with long shoots was observed on media containing (mg l^-1) 2·0 BA with 0·1 or 0·5 NAA and 4·0 BA with 0·1 NAA; with 6·4 and 6·7 mean number of shoots, respectively. TDZ also induced multiple shoots but most of the shoots were stunted. Microshoots were rooted better on half-MS salts supplemented with Indole-3-butyric acid (IBA). The highest rooting percentage (80%) and root number (4·9 roots/microshoot) were promoted by 4·0 mg IBA. The plantlets (90%) successfully survived acclimatization ex vitro.     

Soil seed flora, germination and regeneration pattern of woody species in an Acacia woodland of the Rift Valley in Ethiopia

The objectives of this study were to determine the composition, density and spatial distribution of the soil seed bank of woody species, as well as their regeneration pattern in two different land use systems, controlled (ranch) and open grazing, in an Acacia woodland of the Rift Valley in Ethiopia. We also compared the species composition of the soil seed bank and the above-ground vegetation to find out if differences exist in the soil seed bank and advance regeneration between the two land use systems. The germination requirements of seeds of the woody species were also investigated under laboratory conditions. Acacia senegal, Acacia seyal, Acacia tortilis, Dichrostacys cinerea and Balanites aegyptiaca were encountered in the above-ground vegetation in both systems. Seeds of only A. tortilis (90±32 seeds m⁻²) from the ranch, and A. senegal (5±3 seeds m⁻²) and A. tortilis (72±34 seeds m⁻²) from the open system were found in the soil seed bank along transects with patchy horizontal pattern. The two systems were not significantly different in density of soil seed banks of A. senegal andA. tortilis . Jaccard's similarity index showed that only a few woody species were common in the soil seed flora and above ground vegetation. However, all of the species accumulated seeds (58±43–331±130 seeds m⁻²) in/on the soil under the canopy. Very large numbers of seeds of A. tortilis (19382±9722 seeds m⁻²) and D. cinerea (1278±494 seeds m⁻²) were also found in barns. Most of the seeds recovered from the soil samples (60–80%) were found in the litter layer. Acid and mechanical scarification improved legume germination (36–99% and 60–99%, respectively) over boiling water (0–48%). Treatment means differed significantly for all the legumes (p<0·001) but not for Balanites. Height and diameter class distribution of regeneration of A. tortilis and D. cinerea in both systems and A. senegal in the open system had a negative exponential correlation (r_s=−0·5, −0·25 and −0·86, respectively). A. seyal and B. aegyptiaca showed poor regeneration. Horizontal distribution of advance regeneration of all the species was patchy. Advance regeneration of A. seyal, A. tortilis and B. aegyptiaca were not significantly different, while that of A. senegal and D. cinerea were significantly different between the two systems. Poor representation of species in the soil seed bank along transects and in the different height and diameter classes may be attributed to the low density of mature trees as well as the mode and strategy of seed dispersal. Ungulate and wind dispersed species (e.g. A. senegal, A. tortilis and D. cinerea) were highly favoured. Patchiness in the distribution of seeds and advance regeneration was also a result of endozoochory. Dispersal of non-ungulate dispersed seeds (A. senegal, A. seyal and B. aegyptiaca) was restricted to the canopy zone. Piles of seeds ofA. tortilis and D. cinerea that were found in barns were a result of consumption of their pods by cattle. High concentration of seeds in the litter layer may be due to low soil disturbance and larger size of seeds. The height and diameter class distribution of A. senegal (in the open system), A. tortilis and D. cinerea also indicated that the species have good regeneration. Results from the germination tests indicated that seeds of the legumes require pre-sowing treatments to give a rapid, uniform and improved germination. Intervention through artificial regeneration should be employed to improve the density and regeneration capacity of those species with hampered regeneration at both systems.     

Environmental influences on grafting success of Prosopis ruscifolia (vinal) onto Prosopis alba (algarrobo blanco)

Prosopis ruscifolia, with its 15 cm long spines, has spread onto vast areas of Argentine grazing lands and formed dense impenetrable stands. We examined grafting the highly respected Prosopis alba (algarrobo blanco) onto Prosopis ruscifolia (vinal) rootstock, with the objective of converting undesirable stands of vinal into desirable stands of P. alba. This grafting experiment examined five dates and three light treatments (full-sunlight, half-shade and ‘tent’ treatment 30×40 cm black plastic shade). The full-sun, half-shade and tent treatments gave 2·4±2·02, 6·0±1·22 and 6·6±0·99 (mean±95% confidence interval) successful grafts out of 10, respectively. Future work should evaluate grafting of P. ruscifolia coppice resprouts with P. alba scions, after the P. ruscifolia trees have been harvested for fuelwood or lumber. EliteP. alba clones are now available for use in grafting.     

Size fraction and class composition of phytoplankton in the Antarctic marginal ice zone along the 140°E meridian during February–March 2003

We investigated the size fraction and pigment-derived class compositions of phytoplankton within the euphotic zone of the Antarctic marginal ice zone between 63.3°S and 66.5°S along the 140°E meridian on two consecutive cruises in the late austral summer and early austral autumn of 2003. We observed significant temporal and spatial variations in phytoplankton size and taxonomic composition, although chlorophyll a concentrations were generally below 1 μg l⁻¹ during both periods. Microphytoplankton (>20 μm), mainly diatoms, were prominent in the euphotic zone in the southernmost area around 66.5°S during late summer. In the rest of the study area during both cruises, the phytoplankton community was dominated by pico- and nano-sized populations (<20 μm) throughout the euphotic zone. The small-size populations mostly consisted of diatoms and haptophytes, although chlorophytes were dominant in extremely cold water (−1.5°C) below the overlying warm water around 65.5°S during late summer. From late summer to early autumn, chlorophytes declined in abundance, probably due to increasing temperature within the euphotic zone (−1 to 0°C). These pico- and nano-phytoplankton-dominated populations were often accompanied by relatively high concentrations of ammonium, suggesting the active regeneration of nutrients within the small-size plankton community.