Morphological changes and allometric growth in hatchery-reared Chinese loach <Emphasis Type="Italic">Paramisgurnus dabryanus</Emphasis> (Dabry de Thiersant, 1872)

The length-weight relationship and allometric growth patterns of hatchery-reared Chinese loach, Paramisgurnus dabryanus (Dabry de Thiersant, 1872), were determined from hatching to 60 days after hatching. A best power model was estimated for the length-weight relationship during the early life stages. Positive allometric growth for the head segment, trunk length, tail length and eye diameter was also found in the early life stages, while body depth, tail depth, tail fin length, pectoral fin length and barbel length displayed a negative coefficient. During the subsequent early developmental stage, the growth coefficients showed a clear and common tendency towards isometry for all measured body ratios. The allometric growth changes in Chinese loach during the early stage are possibly the result of selective organogenesis directed towards survival priorities.     

Ontogenetic development in the morphology and behavior of loach （Misgurnus anguillicaudatus） during early life stages

Loach （Misgurnus anguillicaudatus） is a commercially important fish in China and an ideal aquaculture species. However, culturists experience high larval and juvenile mortality during mass production. To provide insight into ways to improve larviculture techniques, we describe the morphological characteristics and behavior of loach during the larval and early juvenile stages. Yolksae larvae ranged from 2.8 to 4.0 mm body length （BL） between days 0 to 4; preflexion larvae ranged from 3.6 to 5.5 mm BL between days 4 to 6; flexion larvae ranged from 4.8 to 8.1 mm BL between days 5 and 14; and post-flexion larvae ranged from 7. l to 15.7 mm BL between days 11 to 27; the minimum length and age of juveniles was 14.1 mm BL and 23 d, respectively. Loach are demersal from hatch through to the early juvenile stages. A suite of morphological characteristics （e.g., external gill filament and ventral mouth opening） and behavioral traits have developed to adapt to demersal living. We observed positive allometric growth in eye diameter, head length, head height, and pectoral fin length during the early larval stages, reflecting the priorities in the development of the organs essential for survival. Our results provide a basis for developing techniques to improve the survival of larval and juvenile loach during mass production.     

池养条件下军曹鱼早期发育阶段的生长和摄食

研究了军曹鱼(Rachycentron canadum)早期阶段的生长和摄食特性。结果表明:1~29日龄间,全长与日龄之间呈指数函数相关:y=3.6922e0.0995x(R=0.9913);体重与日龄间呈指数函数相关:y=0.0002e0.276x(R=0.9830);体重与全长之间为幂函数相关:y=5×10-6x2.786(R=0.9957);口径与日龄的关系为直线函数关系:y=0.1034x-0.0044(R=0.9939)。鱼苗早期阶段的生长速度存在显著性差异。军曹鱼早期鱼苗在3~6日龄时主要摄食原生动物、单轮幼虫和轮虫;7~12日龄时主要摄食桡足类无节幼体;13日龄以后主要摄食桡足类成体。仔、稚鱼阶段,军曹鱼的摄食活动呈现明显的昼夜节律,其饱满度指数均在12时前后出现最高峰,日摄食率分别为57.36%和14.78%。     

高产池塘白鲢鱼苗鱼种生长的研究

本文对高产养鱼池塘中白鲢鱼苗鱼种阶段的生长特性和规律进行了研究。经统计分析表明:1、白鲢鱼苗鱼种阶段的生长过程中,全长和时间的关系均呈直线相关(L=a bt)。鱼种全长以7-8两月生长最快。日增长率、瞬时增长率以鱼苗阶段居高,鱼种阶段次之。2、白鲢鱼苗阶段体重和时间呈指数相关(W=ae~(bt)),体重生长之速度(dw/dt)和加速度(d~2w/dt~2)与时间成正比。3、鱼种阶段体重与时间呈“S”形曲线相关(W=K/1 ae~(-bt)),体重的生长则以7～9月间最快。生长曲线拐点在t=-1n(1/a)/b;W=k/2。体重生长之速度和加速度分别呈“正态”和“正弦”曲线形。日增重率、瞬时增重率和相对增重率亦以鱼苗阶段较大,鱼种阶段次之。     

Length-weight relationships for six freshwater fish species in Iran

The aim of this study is to report the length-weight relationship parameters (a and b) for some economically important fish species from Iranian freshwaters. The length-weight relationships (LWR) are calculated for six freshwater fish species collected during 2006 and 2007 in Iran. The values of the exponent b in the LWR (W=aLb) vary between 2.985 and 3.543. The LWR with high correlation coefficient (r2) is significant for all the species. These parameters are of great importance to evaluate the relative condition of populations, biology, species management and their fisheries and stock assessment. The application of the length-weight relationships presented here should be limited to the observed length ranges.     

Predictive ability of genomic selection models for breeding value estimation on growth traits of Pacific white shrimp Litopenaeus vannamei

Genomic selection(GS)can be used to accelerate genetic improvement by shortening the selection interval.The successful application of GS depends largely on the accuracy of the prediction of genomic estimated breeding value(GEBV).This study is a fi rst attempt to understand the practicality of GS in Litopenaeus vannamei and aims to evaluate models for GS on growth traits.The performance of GS models in L.vannamei was evaluated in a population consisting of 205 individuals,which were genotyped for 6 359 single nucleotide polymorphism(SNP)markers by specifi c length amplifi ed fragment sequencing(SLAF-seq)and phenotyped for body length and body weight.Three GS models(RR-BLUP,Bayes A,and Bayesian LASSO)were used to obtain the GEBV,and their predictive ability was assessed by the reliability of the GEBV and the bias of the predicted phenotypes.The mean reliability of the GEBVs for body length and body weight predicted by the dif ferent models was 0.296 and 0.411,respectively.For each trait,the performances of the three models were very similar to each other with respect to predictability.The regression coeffi cients estimated by the three models were close to one,suggesting near to zero bias for the predictions.Therefore,when GS was applied in a L.vannamei population for the studied scenarios,all three models appeared practicable.Further analyses suggested that improved estimation of the genomic prediction could be realized by increasing the size of the training population as well as the density of SNPs.     

Histological Study on the Digestive System Development of Takifugu rubripes Larvae and Juvenile

The digestive tract of Takifugu rubripes during early life stages was studied with light microscopy. At the beginning of hatching, the digestive tract is represented by a simple and undifferentiated straight tube and does not communicate with the exterior, as the mouth and anus are not open yet. At 2 d after hatching, a constriction between intestine and rectum that will become the intestino-rectal valve is visible. During the endogenous feeding phase, the yolk sac is resorbed and the digestive tract becomes functional and differentiated. The liver and pancreas also become apparent at this time. At onset of exogenous feeding （3 d after hatching）, yolk sac resesves are not completely depleted, suggesting a period of mixed nutrition. The digestive tract differentiates fully into b.uccopharynx, esophagus, stomach, intestine and rectum. At 9 d after hatching, the yolk sac reserves are completely depleted. The most noticeable events occurring from 5 to 17 d after hatching are the transformation of the epithelia type, the differentiation of the pneumatic sac, the epithelial cell, gut convolution, mucosal fold increase, and the growth of liver and pancreas. From 18 to 27 d after hatching, the numbers of intestine folds and mucus cells increase progressively. From then on, morphological changes of digestive features are almost completed. At 27 d after hatching, the morphology and function of digestive system are similar to that of the adult.     

不同条件延缓未受精卵激活对鱼类细胞电融合的影响

泥鳅未受精卵先在合成卵巢液、郝氏液中分别浸泡15、20、30min,然后再受精孵化,统计发育情况,结果表明,合成卵巢液可有效延缓未受精卵遇水后被激活的时间,郝氏液对延缓未受精卵被激活也有一定作用,但效果比合成卵巢液差。用合成卵巢液配制不同浓度胰酶处理受体卵进行细胞电融合实验,胰酶质量浓度为5 mg/mL时,融合卵发育至囊胚期的比例比对照组(郝氏液)高2%,统计分析表明该差异是显著的(P<0.05);但发育至原肠期及孵出期的比例则与对照组无显著差异。胰酶质量浓度为2和1 mg/mL时,融合卵发育到各阶段的比例均低于对照组,统计分析表明两者的差异是显著的(P<0.05)。研究表明,溶液种类、胰酶浓度等对电融合卵的发育有明显的影响,但延缓受体卵的激活时间并不能提高电融合卵的出苗率。用郝氏液配制的2 mg/mL的胰酶溶液处理受体卵,可获得较高比例的融合鱼。     

Age,growth,and mortality rate of the yellow goosefish Lophius litulon(Jordan,1902) in the Yellow Sea

With the decline in the most fisheries resources in the Yellow Sea,the yellow goosefish Lophius litulon has increased in commercial and ecological importance in recent years.We studied the length distribution,length-weight relationship,age composition,growth pattern,mortality,and exploitation rates of the yellow goosefish in the Yellow Sea.Total length(TL) of females and males ranged from 173 to582 mm and 178 to 500 mm,respectively.The length-weight relationships were also estimated for females and males.Age classes from 2 to 4 years predominated in the samples.The von Bertalanffy growth function(VB GF),estimated based on non-linear least-squares methodology,showed significant differences between sexes.Females attained a greater estimated asymptotic total length(765 mm TL) compared to males(579 mm TL).The VBGF did not differ significantly between stocks of the northern Yellow Sea and the southern Yellow Sea.Estimated natural instantaneous mortality rate(M) ranged from 0.25/a to 0.33/abased on four age-and length-based methods.Total instantaneous mortality rate(Z) of total samples calculated by the age-based catch curve method was 0.591/a and the average fishing mortality(F) was 0.30/a.Estimated exploitation rate(E) was approximately 0.5,indicating that the population of L.litulon in the Yellow Sea may be sustainable.These results provide a reference for the present status of L.litulon and information for the management.     

STUDY ON EMBRYONIC DEVELOPMENT AND EARLY GROWTH OF TRIPLOID AND GYNOGENETIC DIPLOID LEFI—EYED FLOUNDER, PARALICHTHYS OLIVACEUS （T. et S. ）

The early effects of chromosomal manipulation of eggs and sperm on the yields of trip-loid and gynogenefic diploid larvae of Paralichthys olivaceus were investigated. Triploidy was achieved by cold shocking fen.ilized eggs at 0 - 2℃ for 45 minutes duration 5 minutes after fen.ilization, and the in-duced triploidy rates were 31.2% - 50% and the relative hatching rates were 53.3% - 99%. Gynnge-aetic diploids were obtained when eggs were inseminated with irradiated sperm and cold shocked at 0 -2℃ for 45 minutes duration 5 minutes after fertihzation. The induced gynogenetic diploid rates and the relative hatching rates were 94 % - 96 % and 48.5 % - 68.5 % respectively. The embryonic development of the triploid experimental group and of the gynogenetic diploid experimental group was delayed at first compared with the control group. But from the gastrula stage, it was not delayed anymore. There were no significant differences in the growth of the triploid experimental group larvae and the control group larvae, and in the growth of the gynogenetic diploid experimental group larvae and the control group larvae ac-cording to Student‘s t-test (α = 0.05). The relationship between the early growth of the triploid experi-mental group larvae and that of gynogenetic diploid experimental group larvae was also studied.     

云南洞穴盲金线鲃一新种(鲤形目:鲤科)

描记采于云南省邱北县一地下洞穴盲金线鲃一新种,以其头上骨质突形状如鹰喙命名鹰喙角金线鲃Sinocyclocheilus aquihornes sp.nov.。新种头形及完全退化的眼与分布于云南泸西阿庐古洞的透明金线鲃S.hyalinus Chen et Yang(1994)相近,但有下列特征可区别:1)角形如鹰喙,后者为一向前的直突起;2)侧线完全,侧线鳞为36,后者侧线不完全,侧线鳞孔9-11;3)背鳍起点位于体中点,至吻端的距离等于至尾鳍基的距离;后者背鳍起点位于体中点之后,至吻端的距离大于至尾鳍基的距离。     

2月龄花鲈形态性状与体质量相关性及通径分析

【目的】探明花鲈(Lateolabrax maculatus)早期主要形态性状对体质量的影响程度,为其良种选育提供参考。【方法】采用数理统计方法对500尾2月龄花鲈的全长(X₁)、体长(X₂)、体高(X₃)、头长(X₄)、头高(X₅)、躯干长(X₆)、吻长(X₇)、眼径(X₈)、尾柄长(X₉)、尾柄高(X₁₀)等10个形态性状和体质量(Y)进行测量分析。【结果】相关性分析结果表明,10个形态性状与体质量的相关性均达到了极显著水平(P<0.01)。通径分析结果发现,体高、全长、眼径、躯干长、吻长、体长等6个性状是影响2月龄花鲈体质量的主要形态性状,其中仅体高对体质量的直接作用大于间接作用,为影响体质量的核心变量。决定系数显示,体高对体质量的单独决定系数最大(0.324),全长和体高对体质量的共同决定系数最大(0.287),6个形态性状对体质量的总决定系数为0.947,表明本研究已将影响2月龄花鲈体质量的主要形态性状全部纳入。经逐步回归分析,拟合得到最优线性回归方程为Y=-1.468+1.314X₃+0.160X₁-0.307X₈+0.149X₆+0.305X₇+0.046X₂。【结论】除体质量外,还须将体高作为花鲈早期选育的目标性状。     

新型城镇化的人地耦合异速增长分析方法

在新型城镇化背景下的城市人口规模与用地面积变化具有复杂性,研究两种要素城镇化水平的相对增长关系,有助于探索人地耦合发展的协同态势及变化动因。本文选取广州市2000—2015年的Landsat时序影像与2000、2010和2015年的人口普查与年鉴数据,在街道（乡镇）尺度上计算人口土地城镇化耦合指数识别4种耦合类型,并在此基础上提出年均增长率异速关系拟合方法。研究结果表明：① 2000—2015年广州市街道（乡镇）人口和土地城镇化呈现明显的圈层结构（核心、内、外圈层）特征,4种人地耦合类型的异速增长分别呈现正异速、负异速、负幂律3种形式,证明人地耦合分类的异速增长建模方法有助于分析人地城镇化的动态演化过程;② 基于人地耦合指数的异速标度分析表明,核心圈层—内圈层—外圈层结构上分别形成了高度集约—集约—相对粗放式扩张的发展格局,结合城市更新改造的空间分析,说明这一特色新型城镇化模式在广州具有一定成效,已初步形成了核心圈层与内圈层协同发展的态势,但需要注意防控外圈层的粗放型增长。研究结果可为新型城镇化理论内涵的认知探索和广州市案例的实证研究提供支持。     

济南—邹平地区侵入岩期次划分之我见

作者根据近年来的研究成果,对济南—邹平地区侵入岩的形成时代和期次关系进行了探讨,并提出以下重要观点: 1.根据已有的钾-氩年龄数据,尤其是根据钐-钕等时线年龄,并结合构造控制和侵入围岩等依据认为济南辉长岩体形成时间应是印支期或印支—燕山早期。将该区侵入岩分为印支—燕山早期和燕山晚期两期。并总结出两期岩浆活动的主要地质特征及其相互区别。 2.认为沙沟闪长岩体不是济南辉长岩体的边缘相,而是独立岩体,晚於济南辉长岩体侵入,是印支—燕山早期第二岩浆阶段的产物。 3.重新厘定了章丘—邹平地区侵入岩的期次顺序。认为王家庄石英二长岩体早于环状二长岩墙,是燕山晚期第二岩浆阶段最早形成的岩体。     

Development Study on Some Digestive Enzymes of Takifugu rubripes Larvae and Juvenile

The activities of some digestive enzymes are studied for Takifugu rubripes larvae and juvenile from the first feeding to 27 d after hatching at selected stages of development. The homogenate of whole larvae body is used for enzymatic determination. Activity of acid protease decreases notably during the beginning days after the commencement of completely exogenous feeding and the days before the beginning of the juvenile stage. Alkaline protease specific activity also decreases at metamorphosis, The activities are associated with the morphology of the developing digestive tract. Amylase activity increases before the first feeding, followed by a decreasing and then a rather constant level. Lipase activity remains low during the larvae and juvenile periods. Alkaline phosphatase activity increases gradually. This reflects the development of brush border membranes of enterocytes.     

Ontogeny of the lymphoid organs of Japanese flounder, Paralichthys olivaceus

Histological study on the ontogeny of the lymphoid organs, kidney, thymus and spleen of Japanese flounder, Paralichthys olivaceus, from hatching to 40 d was carried out. The pronephric kidney duct appeared early in hatching although the primordial haemopoietic stem cells were observed within a week after hatching. The spleen was first seen after 8d of hatching. The thymus appeared after 15d, situated near the pronephric kidney. Small lymphoid cells appeared during the later phase of the post-larval stage in the sequence of thymus, kidney and spleen. During the 40d of observations, there were no distinct inner or outer zones in thymus and no red or white pulp in spleen. These results suggest that the nonspecific defense immune system plays a very important role in the early larval stage of Japanese flounder.     

Yield trait improvement of bay scallops following complete diallel crosses between different scallop stocks

This study describes the complete diaUel hybridization between newly introduced bay scallop stock （W） from Canada and local commercial stock （D） grown under laboratory conditions, in China. Larval survival and growth during all life stages （larvae, spat, and adult） were compared among hybrid （DW, WD） and purebred （DD, WW） populations. Significant heterosis was detected for survival during the larval stage （〉 1% of the mid-parent values）. The mean heterosis （Hm） varied in growth throughout the life span. More than 50% of the Hm values were positive and negative in the DW and WD groups, respectively. The influence of maternal effects and mating types （intrapopulation vs. interpopulation crosses） on growth for all life stages was not consistent. Larval survival did not differ significantly （P〉0.05） with maternal effect or mating type. In the harvest stage, shell length （SL）, shell height （SH）, shell width （SW）, and total weight （TW） were larger in the hybrid compared with the inbred groups. Positive Hm values were observed in SL （1.5%）, SW （5.8%）, and TW （12.3%）, and were more significant in the DW groups （6.1%, 4.5%, 6.8%, and 27.2%）. These results suggest that hybridization between two geographic populations is a good tool for improving bay scallop growth. However, unstable heterosis between the two populations requires further study.     

线纹尖塘鳢仔幼鱼的生长发育与池塘育苗研究

线纹尖塘鳢的胚后发育大致分为仔鱼、稚鱼、幼鱼和成鱼期。初孵仔鱼仅2.875mm。前期仔鱼混合营养期短,仅2～3d。后期仔鱼为器官发育、奇鳍条基本形成期,历时10d,平均全长从4.31mm增至8.97mm。稚鱼为器官分化完毕,奇偶鳍条分节、鳞被形成期,历时27～29d,长至平均全长27.79mm。早期幼鱼鳞被等发育完善,进入生长时期。仔鱼开口饵料为150～200μm轮虫,其摄饵大小与口宽呈正相关。15mm后稚鱼可驯饵摄食人工混合料。对仔、稚、幼鱼的生长测定结果显示,全长与日龄呈线性关系L=0.5289t-0.7354(r2=0.9720),体重与日龄呈指数函数关系,W=0.8236e0.1267t(r2=0.9611),全长与体重呈幂函数关系,曲线回归方程W=0.0045L3.2527,b=3.2527,属匀速生长型。采用池塘培水,添加外源饵料生物系列,稚鱼全长15mm时开始驯饵。2002-2005年共培育全长2.2～3.4cm早期幼鱼523.7万尾,仔鱼培育成15mm稚鱼的成活率为26.8%,培育成早期幼鱼的成活率为75.7%。     

四川盆地川西坳陷须三段砂岩储层致密化过程定量模拟

为了明确川西坳陷须三段砂岩储层致密化过程, 定量恢复致密砂岩储层孔隙度演化, 综合利用常规薄片、铸体薄片、扫描电镜和包裹体等资料, 对须三段成岩作用进行了系统分析, 探讨了成岩作用与孔隙度演化之间的关系。在此基础上, 结合地层埋藏史和热演化史研究, 基于效应模拟原则, 即不对具体成岩作用进行模拟, 而是通过地质参数来模拟各种成岩作用的综合叠加结果, 从而建立须三段砂岩孔隙度演化数学模型, 明确砂岩储层致密化过程。研究结果表明: 须三段主要发育岩屑石英砂岩和岩屑砂岩; 砂岩经历压实、胶结、溶蚀等成岩作用, 其中压实作用和胶结作用是主要的破坏性成岩作用, 且一直存在于砂岩储层演化过程中; 溶蚀作用是主要的建设性成岩作用, 且只出现在特定的60~120℃温度窗口。基于对现今砂岩孔隙度与深度关系的观察, 明确砂岩先后经历了压实、胶结作用和溶蚀作用, 以及溶蚀后的压实、胶结作用。因此, 可将孔隙度演化划分为正常压实与胶结阶段、溶蚀增孔阶段和溶蚀后正常压实与胶结3个阶段。此外, 将孔隙度演化分解为孔隙度减小模型和孔隙度增大模型, 这2种模型在孔隙度演化的3个阶段分别叠加, 可得到模拟孔隙度演化的三段式分段函数模型, 进而实现砂岩储层孔隙度演化过程模拟。通过应用该模型对典型砂岩储层孔隙度演化过程的模拟表明, 川西坳陷须三段因压实作用和早期胶结作用影响, 具有早期致密的特点。      

Age, maturation, and population structure of the Humboldt squid Dosidicus gigas off the Peruvian Exclusive Economic Zones

Age, maturation and population structure of the Humboldt squid Dosidicus gigas were studied based on random sampling of the Chinese jigging fishery off the Peruvian Exclusive Economic Zones (EEZ) during 2008–2010. Estimated ages ranged from 144 to 633 days, confirming that the squid is a short-lived species with longevity no longer than 2 years. Occurrence of mature females and hatching in each month indicated that Humboldt squid spawned year-round. Back-calculated hatching dates for the samples were from January 22^nd, 2008 to April 22^nd, 2010 with a peak between January and March. Two size-based and two hatching date-based populations could be defined from mantle length (ML) at maturity and back-calculated hatching dates, respectively. Females matured at a larger size than males, and there was a significant difference in ML at maturity between the two hatching groups (P <0.05). The waters adjacent to 11°S off the Peruvian EEZ may be a potential spawning ground. This study shows the complexity of the population structure and large variability in key life history parameters in the Humboldt squid off the Peruvian EEZ, which should be considered in the assessment and management of this important resource.