Properties and Anti-Ultraviolet Activity of Gallic Acid-Chitosan-Gelatin Mixed Gel

Hydrogel has high water content and structural similarity with natural extracellular matrix.So it has been widely studied and applied in the field of biomedicine.In order to further develop multifunctional hydrogels,we prepared mixed gels with antiultraviolet properties.This study found that the addition of polysaccharides and polyphenols was beneficial to the rheological,me-chanical properties,and biological activity of the protein.Chitosan(CS)could significantly improve the viscoelasticity,hardness,gel strength,thermal stability and crystallinity of gelatin.Interestingly,the addition of gallic acid(GA)could not only provide significant cross-linking effect,improve gel properties and microstructure,but also improve the UV resistance of the mixed gel.     

Isolation and Characterization of Melanin Pigment from Yesso Scallop Patinopecten yessoensis

Melanin is one of the essential compounds in the pigments of molluscan shells.However,the effects of melanin on color variations in molluscs are largely unknown.Our previous study suggests that Yesso scallop Patinopecten yessoensis might contain melanin pigment in the dark brown shell.We therefore isolated melanin from the pigmented shells using hydrochloric acid method,and characterized the types of melanin pigments by spectrophotometry.The purified melanin,which was verified by spectrophotometry scanning and HPLC analysis,showed the typical characteristics of melanin absorption spectra and HPLC chromatograms.The contents of pheomelanin and eumelanin in pigmented shells,which were determined by the linear standard curve of melanin at 405 nm and 350 nm absorbance,were 48.23 ? 1.350 and 157.65 ? 5.905 mg,respectively.The present results indicate that the brown-pigmented shells of scallops comprise approximately 76.6% of eumelanin and 23.4% of pheomelanin,which supports the presence of eumelanin-rich pigment in scallop shells.Therefore,the combination of hydrochloric acid extraction and spectrophotometric quantification is a rapid and efficient method to isolate and quantify melanin in shells.This will facilitate the melanin studies related to shell color polymorphism and the selective breeding of bivalves with different shell colors.     

Isolation and characterization of melanin pigment from yesso scallop <Emphasis Type="Italic">Patinopecten yessoensis</Emphasis>

Melanin is one of the essential compounds in the pigments of molluscan shells. However, the effects of melanin on color variations in molluscs are largely unknown. Our previous study suggests that Yesso scallop Patinopecten yessoensis might contain melanin pigment in the dark brown shell. We therefore isolated melanin from the pigmented shells using hydrochloric acid method, and characterized the types of melanin pigments by spectrophotometry. The purified melanin, which was verified by spectrophotometry scanning and HPLC analysis, showed the typical characteristics of melanin absorption spectra and HPLC chromatograms. The contents of pheomelanin and eumelanin in pigmented shells, which were determined by the linear standard curve of melanin at 405 nm and 350 nm absorbance, were 48.23 ± 1.350 and 157.65 ± 5.905 mg, respectively. The present results indicate that the brown-pigmented shells of scallops comprise approximately 76.6% of eumelanin and 23.4% of pheomelanin, which supports the presence of eumelanin-rich pigment in scallop shells. Therefore, the combination of hydrochloric acid extraction and spectrophotometric quantification is a rapid and efficient method to isolate and quantify melanin in shells. This will facilitate the melanin studies related to shell color polymorphism and the selective breeding of bivalves with different shell colors.     

珠母贝糖胺聚糖胶囊制备与检测

以马氏珠母贝糖胺聚糖粗品为主要功能成分,以β-环糊精为辅料,制成珠母贝糖胺聚糖胶囊,并进行检测。结果表明:该胶囊中水分、总糖、糖胺聚糖(GAG)和粗蛋白质量分数分别为8.85%、85.23%、3.00%和3.60%;胶囊的装量差异与崩解时限均符合质量标准;胶囊制剂中细菌总数小于1000 g-1,大肠杆菌MPN小于30 g-1,霉菌和酵母菌总数小于25 g-1,符合《保健食品通用卫生要求》规定;致病菌均未检出。     

Investigating the Effects of Greenhouse Vegetable Cultivation on Soil Fertility in Lhasa,Tibetan Plateau

The area of land utilized for growing vegetables in greenhouses has expanded rapidly on the Tibetan Plateau over recent decades. However, the effects of greenhouses on soil fertility as well as variations in these effects between the plateau and plain remain unclear on the Tibetan Plateau. This study assessed the effects of vegetable greenhouses in the vicinity of Lhasa, using open field soil as a control. A total of 92 plough layer(0-20 cm depth) soil samples including 54 from greenhouses and 38 from open fields were taken, and soil pH, electrical conductivity(EC), total soluble salt(TS), soil organic matter(SOM), total nitrogen(TN), available phosphorus(AP), and available potassium(AK) were measured. The results reveal that, soil pH was lower 1.0 units in greenhouses than that in open field. TS was higher 82% and AP was higher 160% overall. Similarly, SOM and TN were higher 32% and 46%, respectively, while AK changed slightly at a higher 1% rate. Results also show that soil properties varied depending on cultivation time and vegetable types. Overall, pH continuously decreased with cultivation time while other soil fertility indicators reached a maximum value after nine years of cultivation before starting to decrease. The effect of leafy vegetable planting on soil was slight overall, while the impact of fruits on soil was more serious. Compared with changes in plain greenhouse soil fertility measured across the eastern China, the effects of greenhouses on soil in Lhasa remain relatively limited; and the change in the degree of soil fertility was lower and the extreme values of soil fertility occurred later in Lhasa.     

Preparation of cationic chitosan-polyacrylamide flocculant and its properties in wastewater treatment

Chitosan derived from crab shells,was used to prepare the graft polymer in aqueous solution with acrylamide (AM) and methacrylatoethyl trimethyl ammonium chloride (DMC) as raw materials and ceric ammonium nitrate (CAN) as initiator.The floc-culation ability of the resulting polymer (PCAD) was studied in waste water treatment experiments.Its properties were determined on the basis of the transmittance of waste water after flocculation.The effects of chitosan and DMC content on PCAD’s flocculation ability wer...     

Advanced glycation endproducts in 35 types of seafood products consumed in eastern China

Advanced glycation endproducts(AGEs) have been recognized as hazards in processed foods that can induce chronic diseases such as cardiovascular disease, diabetes, and diabetic nephropathy. In this study, we investigated the AGEs contents of 35 types of industrial seafood products that are consumed frequently in eastern China. Total fluorescent AGEs level and Nε-carboxymethyl-lysine(CML) content were evaluated by fluorescence spectrophotometry and gas chromatography-mass spectrometry(GC-MS), respectively. The level of total fluorescent AGEs in seafood samples ranged from 39.37 to 1178.3 AU, and was higher in canned and packaged instant aquatic products that were processed at high temperatures. The CML content in seafood samples ranged from 44.8 to 439.1 mg per kg dried sample, and was higher in roasted seafood samples. The total fluorescent AGEs and CML content increased when seafood underwent high-temperature processing, but did not show an obvious correlation. The present study suggested that commonly consumed seafood contains different levels of AGEs, and the seafood processed at high temperatures always displays a high level of either AGEs or CML.     

Evaluation of the Bioavailability of Metals in Sediment from the Southern Coastal Wetland of the Qiantang Estuary by Using Diffusive Gradients in Thin Films Technique

Metal pollution has become an major issue governing the wetland ecosystem health.The southern coastal wetland of the Qiangtang Estuary are facing unusual perturbation due to rapid development along the embayment in recent decades.This study evaluated the bioavailability of metals(Cu,Pb,Cd,Cr and Zn)in the sediment of the southern coastal wetland of the Qiangtang Es-tuary using diffusive gradients in thin films(DGT)techniques and compared with several methods based on total metal content.The results showed that the contents of Cr,Pb,Cd and Cu in sediment,as detected using DGT,were considerably correlated with the exchangeable fraction and the content in Phragmites australis roots,while a weak correlation was observed for Zn.Therefore,DGT analysis could be used to evaluate the bioavailability and potential risk of Cr,Cd,Pb and Cu for P.australis.Quantitative indices,such as DGT concentration,bioaccumulation in P.australis,geoaccumulation index(Igeo)and potential ecological risk index(RI),revealed that Cd was a major potential ecological risk factor along the southern coast wetland of the Qiantang Estuary,especially in the upstream region,which is potentially more vulnerable to the anthropogenic pollution.     

Simultaneous quantification of greenhouse gas and nitric oxide emissions from subtropical conventional vegetable systems: a 2-site field case study in Sichuan Basin

Quantification of greenhouse gases[nitrous oxide(N₂O)and methane(CH₄)]and nitric oxide(NO)emissions from subtropical conventional vegetable systems through multi-site field measurements are needed to obtain accurate regional and global estimates.N2 O,NO and CH4 emissions from subtropical conventional vegetable systems were simultaneously measured at two different sites with hilly topography in the Sichuan basin,southwest China by using the static chamber gas chromatography technique.Results showed that annual soil N₂O and NO fluxes for the treatment receiving N fertilizer ranged from 6.34-7.71 kg N ha^-1 yr^-1 and 0.69-0.85 kg N ha^-1 yr^-1,respectively,while decreased soil CH4 uptakes by 26.4%as compared with no N fertilizer addition across our two sites of experiment.Overall,the average direct N2 O and NO emission factor(EFd)were 0.71%and 0.12%,respectively,which were both lower than the available EFd for subtropical conventional vegetable systems.This finding indicates that current regional and global estimates of N₂O and NO emissions from vegetable fields are likely overestimated.Background N₂O emissions(3.42-3.62 kg N ha^-1 yr^-1)from the subtropical conventional vegetable systems were relatively high as compared with available field measurements worldwide,suggesting that background N₂O emissions cannot be ignored for regional estimate of N₂O emissions in subtropical region.Nevertheless,the significantly intra-and inter-annual variations in N₂O,CH₄ and NO emissions were also observed in the present study,which could be explained by temporal variations of environmental variables(i.e.soil temperature and moisture).The differences in N₂O and NO EFd and CH₄emissions between various vegetable systems in particular under subtropical conditions should be taken into account when compiling regional or global inventories and proposing mitigation practices.     

Effects of water content on the shear behavior and critical state of glacial till in Tianmo Gully of Tibet,China

Glacial tills are widely distributed in Tibet, China, and are highly susceptible to landslides under intense rainfalls. Failures of the slope during rainfall are closely related to the shear behavior of glacial tills at different moisture conditions. This study investigates the shear behavior and critical state of saturated and unsaturated glacial tills through a series of drained direct shear tests. The tests were conducted on two types of compacted glacial tills with different water contents and total normal stresses. A strain softening mode of failure is observed for all water content conditions accompanied by noticeable dilation. Dilatancy is found to decrease with increasing water content. Unsaturated samples showed increased rates of dilation as water content is decreased for all applied normal stresses a behavior which cannot be predicted well by classical stressdilatancy models. Furthermore, it was found that the Critical State Line(CSL), plotted on the(e-ln) plane, can be used to define the shear behavior of unsaturated glacial tills at different water contents.The CSL of saturated glacial tills run parallel to this line. The experimental results in this study are aimed to provide a basic understanding to the underlying failure mechanisms of glacial tills.     

江西省典型县域经济差异影响因子地理探测研究

地理环境因子对区域经济差异的影响一直是国内外学者关注的重点及热点问题,探究各因子对区域经济差异的影响力,科学揭示各因子的作用机制,将为区域经济发展战略的制定提供重要指导依据。本文利用多元回归和地理探测器等方法,综合探测了地形、地貌、土地利用、交通区位等因子对江西典型贫困县及富裕县县域经济差异的影响,深入对比分析了各因子的影响力、交互作用及指示作用,总结主导影响因子及其在2类县域的演变趋势,提出分类精准施策的建议,进一步深化了对该问题的研究。结果表明：① 所选的9个地理环境因子均对贫困县及富裕县县域经济产生影响,不同类型县域的主导影响因子及不同因子对同一类型县域的影响力均存在差异;② 从主导影响因子来看,贫困县主要受自然条件、地理区位的影响,而富裕县主要受资源丰度、交通区位的影响;③ 对比2种县域经济差异的显著影响因子,发现由贫困地区到富裕地区主导影响因素逐步由自然禀赋等不可控因素向交通、技术等可控因素变化;④ 经济发展过程中,应根据区域经济主导影响因子因地制宜,分类精准施策。同时,利用影响因子的强交互作用性,注重对该类因子的改善,对促进经济发展可起到事半功倍的效果。     

西乡盆地膨胀土特性及判别

西乡盆地膨胀上分为三种类型:Ⅰ类为具有弱膨胀潜势的黄褐—褐黄色膨胀土;Ⅱ类为具有中等膨胀潜势的褐黄—浅棕红色膨胀土;Ⅲ类为具有强膨胀潜势的灰绿—灰白色膨胀土。根据西乡盆地膨胀土的基本常规指标(粘粒粒组含量、液限和塑性指数),与西多盆地膨胀土胀缩特性指标的相关性,应用多变量数学分析建立判别函数式,来判别西乡盆地膨胀土。上述研究对西乡地区的建设具有重要的意义。     

Can a dam type of an alpine lake be derived from lake geometry?A negative result

Glacial lake outburst floods(GLOFs)represent one of the most serious hazard and risk in deglaciating high mountain regions worldwide and the need for GLOF hazard and risk assessment is apparent.As a consequence,numerous region-and nation-wide GLOF assessment studies have been published recently.These studies cover large areas and consider hundreds to thousands of lakes,prioritizing the hazard posed by them.Clearly,certain simplification is required for executing such studies,often resulting in neglecting qualitative characteristics which would need manual assignment.Different lake dam types(e.g.,bedrock-dammed,moraine-dammed)are often not distinguished,despite they control GLOF mechanism(dam overtopping/dam breach)and thus GLOF magnitude.In this study,we explore the potential of easily measurable quantitative characteristics and four ratios to approximate the lake dam type.Our dataset of 851 lakes of the Cordillera Blanca suggests that while variances and means of these characteristics of individual lake types differ significantly(F-test,t-test),value distribution of different geometrical properties can’t be used for the originally proposed purpose along the spectra.The only promising results are obtained for extreme values(selected bins)of the ratios.For instance,the low width to length ratio indicates likely morainedammed lake while the high value of ratio indicating round-shape of the lake indicates increased likelihood of bedrock-dammed lake.Overall,we report a negative result of our experiment since there are negligible differences of relative frequencies in most of the bins along the spectra.     

Comparison of lipids in organs of the starfish Asterias amurensis associated with different treatments

Lipids were extracted from organs of the starfish Asterias amurensis associated with different treatments(raw-control,boiling and heating),and then analyzed for lipid content,lipid oxidation index,lipid classes and fatty acid composition.Results showed that boiling softened the hard starfish shells,thus facilitating the collection of starfish organs.As compared with raw organs,the boiled organs had lower water content and higher lipid content,possibly due to the loss of water-holding capacity caused by protein denaturation.Both boiling and heating increased the peroxide value(PV),thiobarbituric acid(TBA) value and carbon value(CV) of lipids.Despite slight increases in the content of complex lipids,associated lipid composition had no substantial variations upon boiling and heating.For simple lipids,the content of 1,2-diglyceride decreased in boiled and heated organs,with free fatty acids observed on thin layer chromatography(TLC).However,neither boiling nor heating significantly changed the fatty acid compositions of simple or complex lipids in starfish organs,suggesting that these two treatments had no significant effects on complex lipids in starfish organs.Together,our results indicated that boiling of starfish soon after capture facilitated the handling and extraction of useful complex lipids consisting of abundant glucosylceramide and eicosapentaenoic acid(EPA)-bounded phospholipids.     

基于交通出行链的就医活动识别理论框架与方法体系

交通是人们实现出行目的的重要工具和载体,也是研究城市居民出行目的的重要手段。本文试图采用交通出行数据来识别就医活动目的的行程,以深化交通大数据研究的应用领域。在合并交通出行链的基础上,构建了就医活动识别的理论框架和方法体系,提出6大准则：邻近性准则、出行链闭合准则、单一出行目的准则、时间耦合性准则、路径偶发准则。以北京市为例,基于公交车刷卡和出租车GPS数据,明确就医出行的关键参数与阈值,最终甄别出以就医为目的的交通出行链,并对识别结果进行分析与验证。基于交通出行链的就医活动识别研究可以弥补传统研究中病例数据和问卷数据样本量小和难获取的不足,为就医活动研究提供了新的方法体系,也为基于其他交通出行目的识别研究提供理论和方法借鉴。     

Protective effects of Ecklonia cava extract on the toxicity and oxidative stress induced by hair dye in in-vitro and in-vivo models

Oxidative hair dyes containing p-phenylenediamine(PPD) are reported to induce an allergic reaction by promoting oxidative stress when absorbed through the skin. Despite the associated risk, these hair dyes remain popular owing to their convenience and sharpness of color. This makes it important to minimize the cytotoxicity and oxidative stress induced by PPD-containing hair dyes. Ecklonia cava extract has been evaluated in different studies for its protective effects against external stress in fibroblasts and keratinocytes. Our study was aimed at using in-vitro and in-vivo models to investigate the extract's effects on cytotoxicity of and oxidative stress induced by PPD-containing hair dyes. Analysis of CIEL*a*b* Color space was first used to determine the range of E. cava extract that would not interfere with the coloring ability of the dye upon addition. Subsequently, the set ranges of E. cava extract(5% and 7%) were added to the hair dye and their toxicity assessed by evaluating the viability of fibroblasts and keratinocytes. The effects on developmental phenotypes and induction of oxidative stress by hair dye were evaluated and compared with those of hair dyes containing different contents of E. cava extract using an in-vivo zebrafish model. Our results showed that E. cava extract in hair dye could significantly decrease the cytotoxicity and levels of oxidative stress caused by hair dyes containing PPD in both in-vitro and in-vivo models.These results suggest that the addition of 7% E. cava extract to 250 μg/mL hair dye does not interfere with the coloring ability of the dye while showing significant protective effects against the hair dye. The study proposes that the use of E. cava extract as an adduct to hair dyes containing PPD reduces the cytotoxicity and oxidative stress induced by these hair dyes.     

广饶县蔬菜基地生态地质环境研究

为了解广饶县蔬菜基地生态地质环境质量,通过对水、土环境质量现状进行调查,重点研究了四大蔬菜基地的生态地质环境特征,结果表明四大蔬菜基地生态环境质量相对较好,蔬菜质量也达到无公害标准。发现在花官乡及稻庄镇存在土壤硒元素富集区,大蒜及芹菜中硒元素含量也较高,对引导当地绿色高效生态农业发展具有重大意义。     

Microanalysis and preliminary pharmacokinetic studies of a sulfated polysaccharide from <Emphasis Type="Italic">Laminaria japonica</Emphasis>

A rapid, sensitive and reproducible high performance liquid chromatography (HPLC) method with post-column fluorescence derivatization has been developed to determine the amount of low-molecular-weight sulfated polysaccharide (GFS) in vivo. The metabolism of GFS has been shown to fit a two component model following its administration by intravenous injection, and its pharmacokinetic parameters were determined to be as follows: half-time of distribution phase (t _1/2α)=11.24±2.93 min, half-time of elimination phase (t _1/2β)=98.20±25.78 min, maximum concentration (C _max)=110.53 μg/mL and peak time (T _max)=5 min. The pharmacokinetic behavior of GFS was also investigated following intragastric administration. However, the concentration of GFS found in serum was too low for detection, and GFS could only be detected for up to 2 h after intragastric administration (200 mg/kg body weight). Thus, the bioavailability of GFS was low following intragastric administration because of the metabolism of GFS. In conclusion, HPLC with postcolumn derivatization could be used for quantitative microanalysis and pharmacokinetic studies to determine the presence of polysaccharides in the serum following intravenous injection.     

雷州半岛大珠母贝贝壳凿孔贝类的初步研究

对雷州半岛海域大珠母贝贝壳凿孔贝类进行了调查研究。结果显示:雷州半岛海域大珠母贝贝壳凿孔贝均为小型的双壳类,褶白蚶Acarplicata(Dillwyn)、偏顶蛤Modiolussp.、翘鳞蛤Irusspp.(两种)、单韧穴蛤Vulsella sp.、杯石蛏Lithophaga calyculata(Carpenter)、蝶铰蛤Trigonothracia sp.、短壳肠蛤Botula silicula(Lamarck)、楔形开腹蛤Gastrochaena cuneiformis Spengler等共9种,其中短壳肠蛤(Botula silicula(Lamarck))的出现频率最高,达50%左右,为雷州半岛大珠母贝贝壳凿孔贝类的主要种类;大珠母贝年龄越大,寄生凿孔贝类数量越多;凿孔贝类可以在孔穴中生活较长时间,最长为6a,生长过程中分泌腐蚀性物质腐蚀贝壳,在贝壳内侧形成斑痕,或凿穿贝壳。     

Optimized water vapor permeability of sodium alginate films using response surface methodology

The water vapor permeability （WVP） of films is important when developing pharmaceutical applications. Films are frequently used as coatings, and as such directly influence the quality of the medicine. The optimization of processing conditions for sodium alginate films was investigated using response surface methodology. Single-factor tests and Box-Behnken experimental design were employed. WVP was selected as the response variable, and the operating parameters for the single-factor tests were sodium alginate concentration, carboxymethyl cellulose （CMC） concentration and CaClz solution immersion time. The coefficient of determination （R2） was 0.97, indicating statistical significance. A minimal WVP of 0.389 8 g-mm/（m^2.h.kPa） was achieved under the optimum conditions. These were found to be a sodium alginate concentration, CMC concentration and CaCl2 solution immersion time at 8.04%, 0.13%, and 12 min, respectively. This provides a reference for potential applications in manufacturing film-coated hard capsule shells.