| 钝顶螺旋藻质粒的电镜观察及杂交研究 |
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| 引用本文： | 秦松,童顺,崔武,李亚菲,王希华,武建秋,吴光耀,张培军,曾呈奎.钝顶螺旋藻质粒的电镜观察及杂交研究[J].海洋与湖沼,1994,25(5):560-563. |
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| 作者姓名： | 秦松 童顺 崔武 李亚菲 王希华 武建秋 吴光耀 张培军 曾呈奎 |
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| 作者单位： | 中国科学院海洋研究所!青岛，266071，中国科学院海洋研究所!青岛，266071，中国科学院海洋研究所!青岛，266071，中国科学院海洋研究所!青岛，266071，中国科学院海洋研究所!青岛，266071，中国科学院海洋研究所!青岛，266071，北京大学生物系!北京，100871，北京大学生物系!北京，100871，中 |
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| 基金项目： | 中国科学院院长基金，中国科学院海洋研究所所长基金 |
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| 摘 要： | 于１９９２年６月－１９９３年４月，对钝顶螺旋藻质粒进行透射电镜观察及分子杂交研究。结果表明，利用层分子膜展开技术，能够观察到超螺旋以及闭合环形的质粒图象；Ｓ６质粒大小的平均值约为２．０ｋｂ，与电泳检测的结果稍有出科。斑点杂交结果表明，不同形态藻株Ｓ６、Ｆ３、Ｆ３弯与Ｆ３直质粒之间具有一定同源性，与染色体ＤＮＡ之间均显示同源性。对钝顶螺旋藻质粒的进一步研究将推动其基因工程育种的开展。
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| 关 键 词： | 蓝藻 钝顶螺旋藻 质粒 电子显微镜 |
| 收稿时间： | 1994/3/16 0:00:00 |
| 修稿时间： | 5/4/1994 12:00:00 AM |
TEM OBSERVATION AND HYBRIDIZATION STUDY OF PLASMID FROM SPIRULINA PLATENSIS |
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| Qin Song,Tong Shun,Cui Wu,Li Yafei,Wang Xihu,Wu Jianqiu,Wu Guangyao,Zhang Peijun and Zeng Chengkui.TEM OBSERVATION AND HYBRIDIZATION STUDY OF PLASMID FROM SPIRULINA PLATENSIS[J].Oceanologia Et Limnologia Sinica,1994,25(5):560-563. |
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| Authors: | Qin Song Tong Shun Cui Wu Li Yafei Wang Xihu Wu Jianqiu Wu Guangyao Zhang Peijun and Zeng Chengkui |
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| Institution: | Institute of Oceanology Academia Sinica, Qingdao 266071;Institute of Oceanology Academia Sinica, Qingdao 266071;Department of Biology, Beijing University, Beijing 100871;Institute for Liver Diseases, PLA, Beijing 100700;Institute of Oceanology Academia Sinica, Qingdao 266071;Institute of Oceanology Academia Sinica, Qingdao 266071;Department of Biology, Beijing University, Beijing 100871;Institute of Oceanology Academia Sinica, Qingdao 266071;Institute of Oceanology Academia Sinica, Qingdao 266071 |
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| Abstract: | Transmission electron microscopic observation and hybridization study of the Plasmid from Spirulina platensis were carried out from June, 1992 to April, 1993.Supercoiled and closed circular patterns were observed under a transmission electron Wicroscope. The size of the plasmid from S6 strain was about 2.0kb, which Was slightly different from the result of electrophoresis. Results of dot blotting show that plasmids from strains of S6, F3, F3 spiral and F3 stfaight differing in form are komologous to each other and are also homologous to chromosomal DNAs. |
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| Keywords: | Cyanophyte Spirulina platensis Plasmid |
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