| 基于多测点LA-ICPMS的耳石核区元素指纹分析技术及其在鱼类群体识别中的实证研究 |
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| 引用本文： | 窦硕增,天野洋典,于 鑫,曹 亮,白井厚太郎,大竹二雄,塚本勝巳. 基于多测点LA-ICPMS的耳石核区元素指纹分析技术及其在鱼类群体识别中的实证研究[J]. 海洋与湖沼, 2011, 42(6): 771-778 |
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| 作者姓名： | 窦硕增 天野洋典 于 鑫 曹 亮 白井厚太郎 大竹二雄 塚本勝巳 |
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| 作者单位： | 中国科学院海洋研究所海洋生态与环境科学重点实验室;东京大学大气海洋研究所 大槌国际沿岸海洋研究中心;中国科学院海洋研究所海洋生态与环境科学重点实验室;中国科学院研究生院;中国科学院海洋研究所海洋生态与环境科学重点实验室;东京大学大气海洋研究所 大槌国际沿岸海洋研究中心;东京大学大气海洋研究所 大槌国际沿岸海洋研究中心;东京大学大气海洋研究所 |
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| 基金项目： | 国家自然科学基金项目资助, 40676086 号, 40976084 号, 41121064 号 |
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| 摘 要： | 构建了多测点激光剥蚀取样（LA）-ICPMS元素分析-剥蚀点显微结构验证的耳石核区元素指纹分析技术,以此进行了五个刀鲚群体（辽东湾、渤海湾、黄河口、胶州湾及长江口群体）识别的实证研究。结果发现,耳石核区Sr：Ca和Ba：Ca比值是有效识别各地理群体的元素指纹,基于二者的各群体判别成功率在46.2%—92.3%之间,总体...
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| 关 键 词： | 耳石 微化学 刀鲚 群体识别 激光剥蚀 质谱分析 |
| 收稿时间： | 2011-09-24 |
| 修稿时间： | 2011-10-25 |
MULTIPLE LASER ABLATIONS ON OTOLITH NUCLEI FOR ICPMS TO ELEMENTALLY FINGERPRINT FISH STOCKS: A CASE STUDY |
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| DOU Shuo-Zeng,AMANO Hironori,YU Xin,CAO Liang,SHIRAI Kotaro,OTAKE Tsuguo and TSUKAMOTO Katsumi. MULTIPLE LASER ABLATIONS ON OTOLITH NUCLEI FOR ICPMS TO ELEMENTALLY FINGERPRINT FISH STOCKS: A CASE STUDY[J]. Oceanologia Et Limnologia Sinica, 2011, 42(6): 771-778 |
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| Authors: | DOU Shuo-Zeng AMANO Hironori YU Xin CAO Liang SHIRAI Kotaro OTAKE Tsuguo TSUKAMOTO Katsumi |
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| Affiliation: | Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences;International Coastal Research Center, Atmosphere and Ocean Research Institute, The University of Tokyo;Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences;Graduate School, Chinese Academy of Sciences;Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences;International Coastal Research Center, Atmosphere and Ocean Research Institute, The University of Tokyo;International Coastal Research Center, Atmosphere and Ocean Research Institute, The University of Tokyo;Atmosphere and Ocean Research Institute, The University of Tokyo |
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| Abstract: | Elemental fingerprinting on otolith nuclei by laser ablation ICPMS (five laser ablations on the nucleus of each otolith) was conducted to delineate tapertail anchovy (Coilia nasus) stocks from five Chinese estuaries. Results showed that otolith Sr : Ca and Ba : Ca ratios were inter-site distinct enough to be used as natal tags for discriminating among geographical stocks with an overall classification accuracy rate of 75.8% (ranging from 61.5% to 84.6% for each stock by discriminant function analysis). Clear images of the concentric rays from the core outwards on the otolith sections could help us precisely locate the otolith core and conduct the five laser ablations. Subsequent microstructure checking confirmed that all the laser ablations were valid, which reduced the errors in elemental fingerprinting caused by potential invalid ablations and elemental data. The anadromous tapertail anchovy showed high fidelity to natal river for spawning and resided in the local estuary for growth, largely decreasing stock mixing throughout their life time. Meanwhile, the less varied environments in their birth rivers in the spawning season could reduce the ambient effects on the elemental uptake onto the otoliths and thus could produce relatively stable microchemistry composition in the otoliths. These ecological and physiological traits of the tapertail anchovy as well as the improvement of laser ablating method increased the accuracy and efficiency of elemental fingerprinting for stock discrimination obtained in the present study. |
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| Keywords: | Otolith Microchemistry Tapertail anchovy Coilia nasus Stock discrimination Laser ablation Mass spectrometry |
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