http://www.sciencedirect.com/science/article/pii/S1674987112000023 |
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Authors: | Liang Shen Tandong Yao Baiqing Xu Hongmei Wang Nianzhi Jiao Shichang Kang Xiaobo Liu Yongqin Liu |
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Institution: | 1. Institute of Tibetan Plateau Research,Chinese Academy of Sciences(CAS),Beijing 100085,China 2. State Key Laboratory of Biogeology and Environmental Geology China University of Geosciences,Wuhan 430074,China 3. State Key Laboratory of Marine Environmental Science,Xiamen University,Xiamen 361005,China |
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Abstract: | Ice melt water from a 22.27 m ice core which was drilled from the East Rongbuk Glacier,
Mt. Everest was incubation in two incubation ways: plate melt water directly and enrichment melt water
prior plate, respectively. The abundance of cultivable bacteria ranged from 0e295 CFU mL 1 to
0e1720 CFU mL 1 in two incubations with a total of 1385 isolates obtained. Comparing to direct cultivation,
enrichment cultivation recovered more bacteria. Pigment-producing bacteria accounted for an
average of 84.9% of total isolates. Such high percentage suggested that pigment production may be an
adaptive physiological feature for the bacteria in ice core to cope with strong ultraviolet radiation on
the glacier. The abundances of cultivable bacteria and pigment-producing isolates varied synchronously
along depth: higher abundance in the middle and lower at the top and bottom. It indicated that the middle
part of the ice core was hospitable for the microbial survival. Based on the physiological properties of
the colonies, eighty-nine isolates were selected for phylogenetic analysis. Obtained 16S rRNA
gene sequences fell into four groups: Firmicutes, Alpha-Proteobacteria, Gamma-Proteobacteria,
and Actinobacteria, with the Firmicutes being dominant. Microbial compositions derived from direct
and enrichment cultivations were not overlapped. We suggest that it is a better way to explore the culturable microbial diversity in ice core by combining the approaches of both direct and enrichment
cultivation. |
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Keywords: | Tibetan Plateau Ice core Cultivable bacteria |
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