Sterols in the western North Atlantic Ocean

The sterol concentrations in fourteen surface and nine deep water samples collected from the continental shelf and slope waters of the western North Atlantic and Sargasso Sea ranged from 0.1 to 1.3μ/l seawater. Isolation and structural elucidation by gas chromatography and combined gas chromatography-mass spectrometry show that cholesterol and β-sitosterol (or clionasterol) are the major free sterols in both the surface and deep water. Fucosterol, brassicasterol, 22-dehydrocholesterol, campesterol (or 22,23-dihydrobrassicasterol), 22-methylenecholesterol, norcholestadienol, and stigmasterol (or poriferasterol) are found in lower concentrations at the surface and in the deep sea. Cholesterol is the major sterol ester in both the surface and deep water, while very low concentrations of other sterol esters were found. The ratio of total free sterols to total esterified sterols is approximately two in both the surface and deep water.Marine sources of sterols in seawater include phytoplankton, yeasts, and marine animals such as Crustacea and molluscs. Terrestrial plants also may contribute. Sterol transport to the deep sea may occur by convective overturn and vertical diffusion or from vertical fluxes of large particles from the surface.