分离自铜矿山尾矿区的铁还原菌nju-T1菌株:菌种鉴定及其还原Fe3+的最适条件

对安徽铜陵狮子山杨山冲尾矿库采集的酸矿水(AMD)及表层尾砂样品,采用FeTo选择性培养基,利用Overlay分离技术,获得了一株具有铁还原功能的细菌nju-Tl.形态观察、生理生化鉴定以及16S rRNA基因序列同源性分析结果表明,该菌株属于Acidithobacillus属.平板培养和液体培养实验证明,nju-Tl...

A Study on the Ferric Reduction Microorganism nju-T1 Isolated from Shizishan Tailings, Tongling, Anhui Province

A new iron reduction strain, named nju-T1, was isolated from acid mine drainage (AMD) and surface tailings samples
from Yangchongshan tailings zone, Shizishan, Tongling, Anhui province, with FeTo selective medium and overlay culture
technology. The morphological, physiological and biochemical research and phylogenetic analysis based on the 16S rRNA gene
sequence showed that the strain belongs to Acidithiobacillus. It was found that nju-T1 could reduce Fe3+ under aerobic conditions
with plate and liquid cultural tests. The pH range for growth was pH 2.0-6.0, with optimum growth at pH 4.5, optimum growth
temperature at 30 ℃ and optimum ferric iron concentration at 5 mmol/L. It was inferred that the reduction sites may be located on
the cell outer membrane. AMD and tailings were controlled by the dynamic balance between the microbial functional groups and
surroundings. The nju-T1 reduced ferric to ferrous, which provided initial energy to ferrous-oxidizing bacteria and maintained the
Fe recycle and stability of acid mine environment. The research on mechanism of ferric reduction with nju-T1 not only broadens
the knowledge about ferric reduction bacteria mainly growing under the anaerobic condition, but also has great significance in understanding the formation of acid mine environment, its development mechanism and its further environmental treatment.