A technique for the molecular genetic analysis of Daphnia resting eggs from sub-recent lake sediments

The Polymerase Chain Reaction (PCR) was employed to facilitategenetic analyses of Daphnia ephippial egg banks fromsediment sequences from lakes Windermere and Esthwaite in the English LakeDistrict, UK. We present a robust DNA extraction technique for resting eggs andan account of genetic characterisation of dated resting eggs from sedimentcores using both mitochondrial and nuclear DNA markers. We also providepreliminary results from genetic analysis of egg banks preserved in shortsediment cores. PCR was employed to facilitategenetic analyses of Daphnia ephippial egg banks collected from both lakes. Thelong-term environmental data available from lake records can provide a backdropagainst which molecular genetic-environmental interactions over time can beexamined, providing a technique for direct assessments of population responseto environmental change in paleolimnological studies of biodiversityhistory.