| 日本绒螫蟹放流群体12S rRNA序列研究 |
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| 引用本文： | 高天翔,王溪宏,张秀梅. 日本绒螫蟹放流群体12S rRNA序列研究[J]. 海洋湖沼通报, 2000, 0(1): 47-51 |
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| 作者姓名： | 高天翔 王溪宏 张秀梅 |
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| 作者单位： | 1. 青岛海洋大学水产学院,青岛,266003
2. 辽宁省海洋渔业开发中心,大连,116015 |
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| 基金项目： | 中国科学院资助项目，39970578， |
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| 摘 要： | 参考果蝇与蚤状溞序列进行了日本绒螯蟹放流群体的线粒体DNA 12S rRNA基因片段的引物设计、PCR扩增及序列测定,得到457bp的碱基序列,其A、T、G、C含量分别为158bp(34.57%)、178bp(38.95%)、51bp(11.16%),70bp(15.32%),与果蝇与蚤状相同基因片段序列含量相似.
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| 关 键 词： | 日本绒螯蟹 放流群体 线粒体DNA 12S rRNA序列 |
| 文章编号： | 1003-6482(2000)01-0047-05 |
SEQUENCE STUDY ON 12S rRNA REGION OF STOCKED POPULATION OF JAPANESE MITTEN CRAB, ERIOCHEIR JAPONICA. DE HAAN |
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| GAO TianxiangWANG Xihong. SEQUENCE STUDY ON 12S rRNA REGION OF STOCKED POPULATION OF JAPANESE MITTEN CRAB, ERIOCHEIR JAPONICA. DE HAAN[J]. Transaction of Oceanology and Limnology, 2000, 0(1): 47-51 |
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| Authors: | GAO TianxiangWANG Xihong |
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| Affiliation: | GAO Tianxiang(Fishery College, Ocean University of Qingdao, Qlngdao, 266003)WANG Xihong(Marine Fisheries Development Center of Liaoning Province, Dalian, 116015) |
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| Abstract: | The primers of mitochondrial DNA 125 rRNA region of stocked population of Japanese mitten crab were designed by using the sequences of Drosophila yaknba and Daphnia pulex. The 457 base pairs that encode the 125 rRNA region were amplified and sequenced. The A, T, G, C contents of the sequences were 158bp(34.57%), 178bp (38.95%), 51bp(11.16%), and 70bp(15.32%) respectively, which are similar with the same region of Drosophila yakuba and Daphnia palex. |
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| Keywords: | Eriocheir japonica stocked population mitochondrlal DNA 12S rRNA sequences |
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