矛尾复虾虎鱼溃疡病病原创伤弧菌的鉴定

2009年8月江苏连云港赣榆县多家虾蟹养殖塘混养的矛尾复虾虎鱼（砂nechogobius hasta）大量死亡．从病鱼深层溃烂组织及肝脏中分离出大量优势生长的细菌，人工感染试验证明分离菌对虾虎鱼的半数致死量（LD，0）为1．63×10^6cfu／g。对分离菌进行了形态特征、理化特性和分子生物学等方面的分析。一方面．分离...

Identification of Vibrio vulnificus from Synechogobius hasta with canker disease

In August 2009, mass mortality of Synechogobius hasta was observed in the shrimp and crabs pond of Ganyu county, Lianyungang city, Jiangsu province. Some pathogenic organisms were isolated from the liver and deep ulceration of the diseased S. hasta. The dominant bacterial strain was Gram-negative, oxidase positive, and fermented glucose to produce acids. In addition, 16S rRNA and gyrB genes analysis showed that isolation was closely related to Vibrio vulnificus than other bacterium. The sequences similarity coefficients were 0.99, on the other hand, and the phylogenetic trees strongly supported isolation were grouped with some reported V. vulnificus. Moreover, the LD₅₀ of the bacteria to S. hasta was 1.63×10⁶ cfu/g. We concluded that V. vulnificus was an important pathogenic bacteria causing canker disease in S. hasta in Ganyu county, Lianyungang city, Jiangsu province.