| 海南地区溶藻弧菌遗传多样性的RAPD分析 |
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| 引用本文： | 欧阳吉隆,周永灿,吴学贵,戴小连,王世锋,谢珍玉.海南地区溶藻弧菌遗传多样性的RAPD分析[J].海洋科学,2011,35(7):30-36. |
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| 作者姓名： | 欧阳吉隆 周永灿 吴学贵 戴小连 王世锋 谢珍玉 |
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| 作者单位： | 海南大学海南省热带水生生物技术重点实验室,海南海口,570228 |
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| 基金项目： | 国家科技支撑计划项目(2009BAB44B02); 国家自然科学基金项目(30760190, 31060360); 霍英东教育基金会高等院校青年教师基金基础性研究课题(121030); 农业科技成果转化项目(2009GB2E200302) |
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| 摘 要： | 以来源于泰国的菌株TG06003作对照，对采自海南地区的27株溶藻孤菌（Vibrio alginolyticus）环境菌株进行了随机扩增多态性DNA标记（RAPD）分析，其中6株为毒力菌株，另外22株为非毒力菌株。实验共得到70个多态性条带，多态性比率（PP13）为98．6％。基因多样度的算术平均数和加权平均数分别为0...
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| 关 键 词： | 溶藻弧菌(Vibrio alginolyticus) RAPD 毒力菌株 遗传多样性 |
| 收稿时间： | 2010/9/12 0:00:00 |
| 修稿时间： | 2011/3/21 0:00:00 |
RAPD analysis of genetic diversity of Vibrio alginolyticus isolated from Hainan province |
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| OU YANG Ji-long,ZHOU Yong-can,WU Xue-Gui,DAI Xiao-lian,WANG Shi-feng,XIE Zhen-yu.RAPD analysis of genetic diversity of Vibrio alginolyticus isolated from Hainan province[J].Marine Sciences,2011,35(7):30-36. |
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| Authors: | OU YANG Ji-long ZHOU Yong-can WU Xue-Gui DAI Xiao-lian WANG Shi-feng XIE Zhen-yu |
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| Institution: | OU YANG Ji-long,ZHOU Yong-can,WU Xue-Gui,DAI Xiao-lian,WANG Shi-feng,XIE Zhen-yu(Hainan University,Key Laboratory of Tropical Aquatic Biotechnology of Hainan Province,Haikou 570228,China) |
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| Abstract: | Taking the Thai strain TG06003 as a control,27 strains of Vibrio alginolyticus collected from Hainan maricultural environment were analyzed using the random amplified polymorphic DNA marker(RAPD) method.Six strains were virulent and the other 22 strains were non-virulent.Seventy polymorphic bands were produced by PCR with eight selected primers,and the PPB was 98.6%.The arithmetic average and the weighted average of gene diversity were 0.922 and 0.454 4,respectively,and the arithmetic average and the weight... |
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| Keywords: | Vibrio alginolyticus RAPD virulent strains genetic diversity |
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