Preliminary analysis of population genetic diversity of cultivated <Emphasis Type="Italic">Laminaria japonica</Emphasis> sporophyte via AFLP technique |
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Authors: | Heng Yi Zhenghong Sui Zhenmin Bao |
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Institution: | 1. Key Laboratory of Marine Genetics, Ministry of Education, Ocean University of China, Qingdao266003, P. R. China;Yunnan Henderson Foreign Language School, Kunming 650000, P. R. China 2. Key Laboratory of Marine Genetics, Ministry of Education, Ocean University of China, Qingdao266003, P. R. China |
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Abstract: | The amplified fragment length polymorphic DNA (AFLP) technique was adopted to estimate the population genetic polymorphism
among 30 sporophytes of Laminaria japonica collected from a cultivating farm in Rongcheng, China. Three methods were used for genomic DNA extraction from Laminaria japonica sporophyte and only the products obtained using the improved genomic DNA extraction kit method proved qualified for AFLP
analysis. The parameters of the method were optimized. Samples of forty milligrams and the cell lysis time of 120 min were
suggested to replace the parameters recommended by the manufacturer. Thirty individuals of Laminaria japonica from the same cultivating site were investigated using one pair of selective primers. A total of 21 loci were obtained and
17 of them were polymorphic. The mean percent age of polymorphic loci of this population was 80.95%. The Nei’s gene diversity
(H) within this population was 0.3028 and the average Shannon’s Information index (I) was 0.4498. A genetic distance matrix
among different individuals was constructed as well. Through this study, an applicable AFLP genetic analysis working system
for Laminaria japonica sporophyte was established. The results of this research also revealed a high level of genetic diversity within the studied
population. |
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Keywords: | sporophyte Laminariajaponica AFLP genetic diversity |
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