Carbon-isotopic analysis of individual pollen grains from C3 and C4 grasses using a spooling-wire microcombustion interface |
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Authors: | David M. Nelson Feng Sheng Hu Jill A. Mikucki Ann Pearson |
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Affiliation: | a Institute for Genomic Biology, University of Illinois, 1206 W. Gregory Drive, Urbana, IL 61801, USA b Department of Earth and Planetary Sciences, Harvard University, 20 Oxford Street, Cambridge, MA 02138, USA c Department of Plant Biology, University of Illinois, 265 Morrill Hall, 505 S. Goodwin Avenue, Urbana, IL 61801, USA d Department of Geology, University of Illinois, 245 Natural History Building, 1301 W. Green Street, Urbana, IL 61801, USA |
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Abstract: | Pollen grains from grasses using the C3 and C4 photosynthetic pathways have distinct ranges of δ13C values that may be used to estimate their relative abundance in paleorecords. We evaluated a spooling-wire microcombustion device interfaced with an isotope-ratio mass spectrometer (SWiM-IRMS) for δ13C analysis of individual grass-pollen grains. Pollen from four C3 and four C4 grass species was isolated through micromanipulation and analyzed as single grains suspended in water. A carbon yield greater than the 2σ range of the carbon content of blanks containing only water was used to distinguish samples containing pollen (“pollen present”) from those not containing pollen. This criterion resulted in the exclusion of ∼45% of the 946 samples applied to the wire. The average δ13C values (±1σ) of the remaining samples were −26.9‰ (±6.3‰) and −11.5‰ (±9.6‰) for C3 grasses and C4 grasses, respectively, after blank-correcting the δ13C data. These results suggest that the SWiM-IRMS system can be used to distinguish C3 from C4 grass pollen. The high variability in measured δ13C values is likely caused by a combination of factors. These include natural isotopic variability among individual pollen grains; the relatively poor precision that can be obtained when determining δ13C values of such small samples; and the uncertainty in the magnitude, isotopic composition, and stability of the analytical blank. Nonetheless, high percentages of individual pollen grains were correctly classified as being of either C3 or C4 origin. On average, 90% (range = 78-100%) of pollen grains from C3 grasses had δ13C values more negative than the cutoff threshold of −19.2‰; while 84% (range = 77-90%) of pollen grains from C4 grasses had δ13C values more positive than −19.2‰. Compared with analysis using an elemental analyzer interfaced with an IRMS (EA-IRMS), the number of pollen grains required for δ13C-based evaluation of C3/C4 grass composition is many times lower with the SWiM-IRMS. Additionally, δ13C data from the SWiM-IRMS does not need to be incorporated into a mixing model to derive estimates of the abundance of C3 and C4 grass pollen. Carbon-isotopic analysis of individual grass-pollen grains using the SWiM-IRMS system may help improve our understanding of the evolutionary and ecological significance of grass taxa in the paleorecord. |
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