Potassium channel in peripheral blood lymphocytes of turbot (Scophthalmus maximus) |
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Authors: | CHAI Yingmei HUANG Xiaohang CONG Bailin LIU Chenlin and LIU Shenghao |
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Institution: | 1.College of Marine Life Sciences, Shandong University at Weihai, Weihai 264209, China;Marine Bioactive Substances Laboratory, the First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China2.Marine Bioactive Substances Laboratory, the First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China |
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Abstract: | ;In order to provide pertinent evidence of ion channel with immune response in the fish, whole cell patch-clamp technique was employed for potassium ion channel study in turbot (Scophthalmus maximus). Lymphocytes were isolated by Percoll density gradient centrifugation from peripheral blood samples, and electrophysiological characters of the channel were analyzed. In the recorded cells, activated voltage of the channels was -42.5±3.7 mV and the average peak current was 313.12±28.2 pA. The channel was identified as voltage dependent, the current was outward and it could be inhibited by 10 mmol/dm~3 TEA or 5 mmol/dm~3 4-AP, a specific potassium channel inhibitor, identifying the existence of potassium channel in peripheral lymphocytes of the turbot. |
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Keywords: | patch-clamp technique potassium channel turbot peripheral blood lymphocyte |
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