| 雨生红球藻紫外诱变及抗铵品系筛选 |
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| 引用本文： | 张丽,范鸣浩,宋益民,张学成.雨生红球藻紫外诱变及抗铵品系筛选[J].中国海洋大学学报(自然科学版),2011,41(6):68-74. |
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| 作者姓名： | 张丽 范鸣浩 宋益民 张学成 |
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| 作者单位： | 1. 青岛科技大学化工学院,山东,青岛,266042;中国海洋大学海洋生命学院,山东,青岛,266003
2. 中国海洋大学海洋生命学院,山东,青岛,266003 |
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| 摘 要： | 经紫外诱变,筛选出在碳酸氢铵浓度为400 mg/L条件下可以生存的突变株。通过培养基中氮、磷、铁、温度、光照及pH值对雨生红球藻诱变株生长的影响进行比较,对以上3种营养盐及3种环境因子分别进行三因素三水平正交实验,以优化培养条件。进而对雨生红球藻野生型及诱变株的生长状况及色素含量进行比较。实验结果表明,雨生红球藻抗铵品系在培养基中NaNO30.1 g/L,KH2PO40.01 g/L,FeCl3.6H2O 1.0 mg/L,在pH值为8.0,光照强度100μmol.m-2.s-1,温度为18℃的培养条件下,雨生红球藻的生物量得到有效提高。
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| 关 键 词： | 雨生红球藻 紫外线 诱变 碳酸氢铵 正交实验 |
UV Mutagenesis and Anti-Ammonium Strain Selection of Haematococcus pluvialis |
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| ZHANG Li,FAN Ming-Hao,SONG Yi-Min,ZHANG Xue-Cheng.UV Mutagenesis and Anti-Ammonium Strain Selection of Haematococcus pluvialis[J].Periodical of Ocean University of China,2011,41(6):68-74. |
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| Authors: | ZHANG Li FAN Ming-Hao SONG Yi-Min ZHANG Xue-Cheng |
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| Institution: | ZHANG Li1,2,FAN Ming-Hao1,SONG Yi-Min1,ZHANG Xue-Cheng2(1.College of Chemical Engineering,Qingdao University of Science and Technology,Qingdao 266042,China,2.College of Marine Life Sciences,Ocean University of China,Qingdao 266003,China) |
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| Abstract: | A Haematococcus pluvialis strain that can stands in 400 mg/L NH4HCO3 was obtained by UV mutagenesis and selection.In order to determine the optimal cultural conditions,effects of nutrition elements(nitrate,phosphate and ferric) and other ecological elements(pH value,temperature and light intensity) on the growth of Heamatococcus pluvialis of mutant strains,and orthogonal tests with three elements and three levels were conducted.Also,the cell growth and photosynthetic pigment content of wild type and mutant ... |
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| Keywords: | Heamatococcus pluvialis ultraviolet mutagenesis strain selection orthogonal tests |
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