NOTE INDUCTION OF OVULATION IN FEMALE MUD SKIPPER (BOLEOPHTHALMUS PECTINIRESTRIS) BY HCG,ANALOGUE OF LHRH AND PIMOZIDE

The effects of two intraperitoneal injections of human chorionic gonadotropin (HCG) or [ D-ALA6, pro9- N- ethylamide]-luteinizing hormone releasing hormone analogue (LHRH-A), respectively, and the combined effect of LHRH-A with pimozide (PIM), a dopamine antagonist, on ovulation in female mud skipper were investigated. A high rate of ovulation (83.3%)was observed after injection with HCG(30 I. U./g). Injection of LHRH-A at a total dosage of 2μg/g was 100% effective in inducing ovulation. No ovulations occurred in the group receiving a low dosage of LHRH-A (0.02μg/g). Injection of LHRH-A(0.2μg/g)with PIM (10 or 20 μg/g)induced greater ovulatory response than injection with LHRH-A (0.2μg/g)alone. PIM at total dosages of 0.2μg/g or 2μg/g did not enhance the potency of LHRH-A to stimulate ovulation.