| 北极黄河站植物根际土壤放线菌分离培养及其鉴定 |
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| 引用本文： | 滕海波,唐学玺,肖慧,俞勇,李会荣.北极黄河站植物根际土壤放线菌分离培养及其鉴定[J].极地研究,2009,21(1):33-42. |
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| 作者姓名： | 滕海波 唐学玺 肖慧 俞勇 李会荣 |
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| 作者单位： | 中国海洋大学海洋生命学院; 国家海洋局极地科学重点实验室; 中国极地研究中心; |
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| 基金项目： | 中国极地战略研究基金 |
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| 摘 要： | 通过稀释涂布平板法,对北极黄河站(挪威新奥尔松地区)7种常见植物根际土壤样品进行放线菌的分离培养。经扩增性rDNA限制性酶切片段分析(ARDRA)及16S rDNA序列分析确定放线菌53株,分别属于8个属,除了优势菌链霉菌属(Streptomyces)和节杆菌属(Arthrobacter)外,还发现了红球菌(Rhodococcus)、糖丝菌(Saccharothrix)、拉塞氏杆菌(Rathayibacter)、微球菌(Micrococcus)、诺卡氏菌(Nocardia)、克里贝拉(Kribbella)等6种稀有菌属。
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| 关 键 词： | 北极 根际土壤 放线菌 16S rDNA 北极 根际土壤 放线菌 16S rDNA |
ISOLATION AND IDENTIFICATION OF ACTINOBACTERIA FROM RHIZOSPHERIC SOIL IN THE ARCTIC YELLOW RIVER STATION |
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| Teng Haibo,Tang Xuexi,Xiao Hui,Yu Yong,Li Huirong.ISOLATION AND IDENTIFICATION OF ACTINOBACTERIA FROM RHIZOSPHERIC SOIL IN THE ARCTIC YELLOW RIVER STATION[J].Chinese Journal of Polar Research,2009,21(1):33-42. |
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| Authors: | Teng Haibo Tang Xuexi Xiao Hui Yu Yong Li Huirong |
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| Institution: | 1.College of Marine Life Science; Ocean University of China; Qingdao 266003; China;2.SOA key laboratory for polar science; Polar Research Institute of China; Shanghai 200136; China |
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| Abstract: | Using spread-plate technique,actinobacterial strains were isolated from rhizospheric soil of 7 plants collected in the Arctic Yellow River Station(Ny-Alesund,the Arctic). 53 actinobacteria were indentified by the analysis of ARDRA and 16S rDNA sequences and were shown to represent strains from 8 genera: Streptomyces and Arthrobacter which is predominant and rare actinobacteria Rhodococcus,Saccharothrix,Rathayibacter,Micrococcus,Nocardia,Kribbella. |
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| Keywords: | 16S rDNA |
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