Marine microgels: Optical and proteomic fingerprints |
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Authors: | Mnica V Orellana Timothy W Petersen Alan H Diercks Samuel Donohoe Pedro Verdugo Ger van den Engh |
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Institution: | aInstitute for Systems Biology, 1441 North 34th Street, Seattle WA 98103, USA;bUniversity of Washington, Department of Bioengineering, Friday Harbor Labs., 620 University Rd., Friday Harbor, WA 98105, USA |
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Abstract: | Dissolved organic matter (DOM) is a major carbon reservoir for the global carbon cycle, and its molecules play a key role in the biogeochemistry of the ocean. Colloidal DOM macromolecules assemble to form polymer hydrogels known as marine microgels. Marine microgels represent one of the most dynamic pools of organic carbon in the ocean. However, their optical characteristics and their contribution to ocean optical properties are largely unknown. In this work, we explore the optical and proteomic properties of spontaneously assembled DOM polymer microgels. Microgels from cultures and from Puget Sound seawater were sorted and counted using a dual-laser (365 nm/365 nm) high-speed cell sorter. This sorter has been adapted to interface with a scanning monochromator to measure the fluorescence emission spectrum of the microgels over the range from 300 to 850 nm. Surprisingly, the microgels show a broad fluorescence emission from 420 to 520 nm when excited with UV light. The microgels were classified according to their blue autofluorescence, and by three criteria that are used to define microgels: 1) staining with chlortetracycline 2) the ability to undergo phase transitions at low pH, and 3) dispersion following calcium chelation by EDTA. |
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Keywords: | Microgels Fluorescence Flow cytometry Proteomics |
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