凡纳滨对虾α-淀粉酶基因PCR-RFLP多态性与生长性状的相关分析

采用PCR-RFLP法对379尾凡纳滨对虾α-淀粉酶基因(AMY)多态性进行检测,并分析AMY基因多态性与生长性状的相关性.结果表明:引物AMY-5与AMY-6扩增片段均具多态性,引物AMY-5扩增产物有CC,CT和TT 3种基因型,AMY-6扩增产物有AA,AB和BB 3种基因型.AMY基因AMY-5引物位点TT基因...

PCR-RFLP polymorphism of alpha-amylase gene and its association with growth traits of Litopenaeus vannamei

The polymorphism of the alpha-amylase (AMY) gene was analyzed by PCR-RFLP method in 379 Litopenaeus vannamei, and association analyses were carried out to evaluate the effects of genotypes of the candidate genes on growth traits in Litopenaeus vannamei. The results showed that both the products amplified by primer AMY-5 and AMY-6 displayed polymorphisms, and there were three genotypes (CC, TT and CT) for AMY-5 and three genotypes (AA, AB and BB) for AMY-6 respectively. The results indicated that there was one single nucleotide mutation (C→T) in the exon5 (123 bp) and one single nucleotide mutation (C→T) in the exon6 (81 bp) of the alpha-amylase gene. The two mutations did not cause any amino acid changes and were silence mutations. Least square was applied to analyze the correlation of different genotypes and the growth traits. The CC and CT genotypes showed significant differences in body mass, body length, carapace length, carapace width, first abdominal segment length and the last abdominal segment length compared with the genotype TT(P<0.05). The AA and BB genotypes showed significant differences in body length, first abdominal segment length and the last abdominal segment length compared with the genotype AB (P<0.05). The AA genotype showed significant differences in body mass, carapace length, carapace width compared with the genotype AB (P<0.05). It implied that the AMY gene could have a major effect on the growth traits of Litopenaeus vannamei, and could be used as the gene that significantly affect the growth traits in Litopenaeus vannamei, and these molecular genetic markers may be applied for Litopenaeus vannamei breeding.