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| 大头鳕和半滑舌鳎RAPD分析条件的优化 | |
| 引用本文： | 鹿志创,高天翔,李玉晖,庄志猛,樱井泰宪.大头鳕和半滑舌鳎RAPD分析条件的优化[J].海洋湖沼通报,2007,44(3):96-103. |
| 作者姓名： | 鹿志创 高天翔 李玉晖 庄志猛 樱井泰宪 |
| 作者单位： | 1. 中国海洋大学水产学院,青岛,266003 2. 中国水产科学研究院黄海水产研究所,青岛,266071 3. 北海道大学水产学部,涵馆,041-8611 |
| 基金项目： | 日本学术振兴会科学研究费辅助金(特别研究员奖励费) |
| 摘 要： | 大头鳕和半滑舌鳎是我国重要的两种经济鱼类,本论文以大头鳕和半滑舌鳎基因组DNA为模板,对RAPD分析中的DNA模板浓度进行了优化,确定最适浓度。并分别筛选出20条和18条扩增结果稳定性好、多态性强的随机引物用于大头鳕以及半滑舌鳎的RAPD遗传变异分析。 |
| 关 键 词： | 大头鳕 半滑舌鳎 RAPD 条件优化 引物筛选 |
| 文章编号： | 1003-6482（2007）03-0096-08 |
| 修稿时间： | 2006-05-22 |
OPTIMIZATION FOR THE RAPD REACTION SYSTEM IN GADUS MACROCEPHALUS TILESIUS AND CYNOGLOSSUS SEMILAEVIS GUNTUER |
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| LU Zhichuang,GAO Tianxiang,LI Yuhui,ZHUANG Zhimeng,SAKURAI Yasunori.OPTIMIZATION FOR THE RAPD REACTION SYSTEM IN GADUS MACROCEPHALUS TILESIUS AND CYNOGLOSSUS SEMILAEVIS GUNTUER[J].Transaction of Oceanology and Limnology,2007,44(3):96-103. | |
| Authors: | LU Zhichuang GAO Tianxiang LI Yuhui ZHUANG Zhimeng SAKURAI Yasunori |
| Institution: | 1. Fishery College, Ocean University of China, Qingdao 266003, China; Yellow Sea Fisheries Researeh Institute, Chinese Aeademy of Fishery Seienees, Qingdao 266071, China; 3. Faeulty of Fisheries, Hokkaido University, Hakodate 041-8611, Japan |
| Abstract: | Gadus rnacrocephalus Tilesius and Cynoglossu sernilaevis Gtintuer are two important economic fishery species of China. The influence of DNA concentration on the results of RAPD amplification was analyzed by using the materials of Gadus maeroeephalus and Cynoglossus sernilaevis Gtintuer DNA. Twenty primers were selected from forty sangon primers for Gadus rnacrocephalUs, and eighteen primers were selected for Cynoglossus semilaevi all of them can generate genetic variation. |
| Keywords: | Gadus macrocephalus Tilesius Cynoglossus semilaevis Gtintuer RAPD optimization selection of polymorphic primers |
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