Explant exenisation for tissue culture in marine macroalgae |
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| Authors: | Liu Xuewu Bernard Kloareg |
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| Institution: | (1) Centre d'Etudes Oceanologiques et de Biologie Marine, CNRS UPR 4601 et Universite Paris VI, BP 74, F-29682 Roscoff Cedex, France |
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| Abstract: | Unialgal explants fromLaminaria digitata, and from a variety of red algae, were obtained by hand removing the visible epiphytes, and stirring the tissue in the presence
of glass beads. Two antibiotic mixtures were found to be efficient in removing the contaminating fungi and bacteria from the
algae. The procedure proved suitable as a primary step in the tissue culture of the investigated species. |
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| Keywords: | unialgal axenic antibiotics disinfectants callus regeneration |
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