| 长耳珠母贝核rRNA基因ITS-2序列分析 |
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| 引用本文： | 何毛贤,黄良民.长耳珠母贝核rRNA基因ITS-2序列分析[J].热带海洋学报,2004,23(5):81-84. |
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| 作者姓名： | 何毛贤 黄良民 |
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| 作者单位： | 中国科学院南海海洋研究所,广东,广州,510301 |
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| 基金项目： | 国家"863"计划项目(2002AA603022),博士后基金资助项目 |
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| 摘 要： | 以相应引物聚合酶链式反应(PCR)扩增长耳珠母贝(Pinctada chemnitzi)核核糖体RNA基因第2转录间隔区(ITS-2),PCR产物大小约500bp,经克隆、测序,所得序列包含5．8S和28SrRNA基因部分序列和ITS-2全序列,4种碱基含量分别为27．11％(A)、24．95％(G)、21．26％(T)和26．68％(C)。5个克隆的DNA序列差异为0．0％-0．4％,不存在个体内变异。对其潜在应用进行了讨论。
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| 关 键 词： | 长耳珠母贝 ITS-2 序列分析 |
| 文章编号： | 1009-5470(2004)05-0081-04 |
| 修稿时间： | 2002年3月17日 |
SEQUENCING OF RIBOSOMAL INTERNAL TRANSCRIBED SPACER 2 OF PINCTADA CHEMNITZI |
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| HE Mao-xian,HUANG Liang-min.SEQUENCING OF RIBOSOMAL INTERNAL TRANSCRIBED SPACER 2 OF PINCTADA CHEMNITZI[J].Journal of Tropical Oceanography,2004,23(5):81-84. |
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| Authors: | HE Mao-xian HUANG Liang-min |
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| Abstract: | The ribosomal internal transcribed spacer 2 (ITS-2) of wild pearl oyster, Pinctada chemnitzi, was amplified via PCR using relevant primers. The size of PCR product was about 500bp DNA. The DNA products were cloned and sequenced. The sequence contained a partial sequence of 5.8S rRNA gene, a complete sequence of ITS-2 and a partial sequence of 28S rRNA gene. The contents of A, G, T and C in ITS-2 sequence were 27.11%, 24.95%, 21.26% and 26.68%, respectively. The alignment comparison indicated that the divergence of ITS-2 sequences form five clones of an individual was 0.0%-0.4%, showing no variability at individual level. The potential application of ITS-2 to phylogenetic research of pearl oysters was discussed, too. |
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| Keywords: | Pinctada chemnitzi internal transcribed spacer 2 (ITS-2) sequencing |
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