双齿围沙蚕的胚胎发育与纤毛虫病害

双齿围沙蚕(Perinereis aibuhitensis)亲体为2010 年半人工育苗的苗种, 人工培育至异沙蚕体后进行集中催产、人工授精, 受精卵经过消毒后, 孵化至囊胚期, 收集入水泥池进行中间培育, 经3 d 孵化至3 刚节幼体再次收集, 放入土池进行幼虫培育。卵子受精后, 定期(早期每隔1 h, 囊胚期后每隔3 h)取受精卵, 置于OLYMPUS－CX31 显微镜进行活体观察并进行拍照。结果表明, 在双齿围沙蚕的胚胎发育过程中, 出现了与已有结论不符的特殊现象, 例如油球与胚胎发育的关系, 胚胎的高温孵化。而且,在孵化期, 一种寄生纤毛虫导致沙蚕胚胎及出膜3 刚节疣足幼体的大量死亡, 其病害机理有待进一步研究。

Embryonic development of and disease of infusorium in Perinereis aibuhitensis

The mature Perinereis aibuhitensis came from the semi-artificial cultured populations in 2010.During July and August,2011,some of the cultured populations was matured into heteronereis.Though the heteronereis can reproduce under artificial conditions when both male and female individuals(30~50) are pooled into one palstic basket,we used artifical fertilizing methods in order to obtain synchronously ontogenitic embryoes,then to faciliate the following larvae-culturing regimes.After disinfection,the fertilizing eggs were transferred into cement pond for middle culture.When the embryo developed into the ontogentic stage of 3-chaetae nectochaete on day 3 post fertilization,the larvae were collected from cement pond,and put into mud pond for last cultivation.The early ontogeny of P.aibuhitensis was observed under Olympus –cx31 microscope.The typical characteristic images were taken using charge-coupled device(CCD) and SONY H-50 camera.There were some phenomena different from previous reports,including numbers of oil globules vs embryonic development,temperature vs embryonic development.One of pathogenic ciliate species emerged during the early development and resulted in mass death of embryo and larvae of P.aibuhitensis.