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| Subunit composition and chromophore content of R-phycocyanin and allophycocyanin from Porphyra haitanensis | |
| 引用本文： | Gao Hongfeng,Cao Wen,Ji Minghou. Subunit composition and chromophore content of R-phycocyanin and allophycocyanin from Porphyra haitanensis[J]. 海洋学报(英文版), 1994, 13(2): 253-259 |
| 作者姓名： | Gao Hongfeng Cao Wen Ji Minghou |
| 作者单位： | Institute of Oceanology, Academia Sinica, Qingdao 266071, China;Institute of Oceanology, Academia Sinica, Qingdao 266071, China;Institute of Oceanology, Academia Sinica, Qingdao 266071, China |
| 摘 要： | ＳｕｂｕｎｉｔｃｏｍｐｏｓｉｔｉｏｎａｎｄｃｈｒｏｍｏｐｈｏｒｅｃｏｎｔｅｎｔｏｆＲ－ｐｈｙｃｏｃｙａｎｉｎａｎｄａｌｌｏｐｈｙｃｏｃｙａｎｉｎｆｒｏｍＰｏｒｐｈｙｒａｈａｉｔａｎｅｎｓｉｓ￥ＧａｏＨｏｎｇｆｅｎｇ；ＣａｏＷｅｎｄａａｎｄＪｉＭｉｎｇ... |
| 关 键 词： | Porphyra haitanensis R-phycocyanin allophycocyanin subunit and chromophore |
| 收稿时间： | 1993-02-26 |
| 修稿时间： | 1994-01-08 |
Subunit composition and chromophore content of R-phycocyanin and allophycocyanin from Porphyra haitanensis |
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| Gao Hongfeng,Cao Wenda and Ji Minghou. Subunit composition and chromophore content of R-phycocyanin and allophycocyanin from Porphyra haitanensis[J]. Acta Oceanologica Sinica, 1994, 13(2): 253-259 | |
| Authors: | Gao Hongfeng Cao Wenda Ji Minghou |
| Affiliation: | Institute of Oceanology, Academia Sinica, Qingdao 266071, China |
| Abstract: | R-phycocyarnin (RPC) of Porphyra haitmmsis (T.J.Chang et B.F.Zheng) was chromatographed on Bio-Rex 70 column with urea solution (pH 3.0) as an eluent, and α and β two subunits were isolated.Their molecular weights were determined on SDS-PAGE at 18 400 and 20 500, respectively, while those of a and β subunits of allophycocyanin (APC) at 18 800 and 19 700, respeMively, and those of RPC and APC were at 117 000 and 122 000, respectively. Both the molar ratio of α and β subunits of RPC and APC were 1:1, and the subunit composition was conf firmed to be(αβ)3. It was ascertained that in RPC a subunit contains one chromophore phycocyanobilin (PCB) and β subunit has one chromophore PCB and one phycoerythrobilin (PEB),while in APC both α and β subunits contain one PCB. |
| Keywords: | Porphyra haitanensis R-phycocyanin allophycocyanin subunit and chromophore. |
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