Simplified technique for the rapid determination of phytoplankton pigments by reverse-phase high-performance liquid chromatography |
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| Authors: | Reiko Suzuki Masayuki Takahashi Ken Furuya Takashi Ishimaru |
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| Affiliation: | (1) Ocean Research Institute, University of Tokyo, Minamidai, 164 Nakano, Tokyo, Japan;(2) Botany Department, Faculty of Science, University of Tokyo, Hongo, Bunkyo, 113 Tokyo, Japan;(3) Faculty of Bioresources, Mie University, Kamihama, 514 Tsu, Japan;(4) Tokyo University of Fisheries, Konan, 108 Minato, Tokyo, Japan |
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| Abstract: | A simplified technique for the rapid analysis of photosynthetic pigments in marine phytoplankton is described. The pigments extracted in N,N-dimethylformamide (DMF) are injected directly into the reverse-phase high-performance liquid chromatograph fitted with a photodiode array detector. We developed a new stepwise program; that is methanol-water (75 25 v/v), methanol, methanol-acetone (8020) and methanol-acetone (6535). This program gives a good separation of at least 13 carotenoids, and 10 chlorophylls and their derivatives, completed within 20 min. The quantitative precision is high; the maximum standard error was less than 6%. The stability of pigments in DMF were also considered for application of this technique to the lab work on board the ship. |
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