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Sequence Comparison of Partial Cytochrome b Genes of Two Coilia species
作者姓名:LIU Jinxian    GAO Tianxiang  WANG Yujiang  and ZHANG Yaping The Key Laboratory of Mariculture  Ministry of Education  Ocean University of China  Qingdao  P.R.China Laboratory of Molecular Evolution and Genome Diversity  Kunming Institute of Zoology  Chinese Academy of Sciences  Kunming  P. R. China
作者单位:LIU Jinxian1),2),GAO Tianxiang1),WANG Yujiang1),and ZHANG Yaping2) 1) The Key Laboratory of Mariculture,Ministry of Education,Ocean University of China,Qingdao 266003,P.R.China 2) Laboratory of Molecular Evolution and Genome Diversity,Kunming Institute of Zoology,Chinese Academy of Sciences,Kunming 650223,P. R. China
基金项目:国家重点基础研究发展计划(973计划)
摘    要:1Introduction Japanese grenadier anchovy,Coilia ectenes Jordanet Seale(1905),is distributed in the northwest Pacif-ic.The congener,Osbeck's grenadier anchovy,Coil-ia mystus(Linnaeus,1758),is widely distributed inthe west Pacific.Both species inhabit coastal watersand estuaries and can reach up to rivers.The twospecies are important contributors to the coastal fish-eries of China(Zheng et al.,2003).Many studies on the fishery biology of the twospecies have been conducted(Zheng et al.,2003)…

关 键 词:细胞色素  b基因  基因转化  线粒体DNA  凤尾鱼

Sequence Comparison of Partial Cytochrome b Genes of Two Coilia species
LIU Jinxian,,GAO Tianxiang,WANG Yujiang,and ZHANG Yaping The Key Laboratory of Mariculture,Ministry of Education,Ocean University of China,Qingdao ,P.R.China Laboratory of Molecular Evolution and Genome Diversity,Kunming Institute of Zoology,Chinese Academy of Sciences,Kunming ,P. R. China.Sequence Comparison of Partial Cytochrome b Genes of Two Coilia species[J].Journal of Ocean University of China,2005,4(1):85-88.
Authors:Liu Jinxian  GAO Tianxiang  WANG Yujiang  ZHANG Yaping
Institution:1. The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, P. R. China;Laboratory of Molecular Evolution and Genome Diversity, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, P. R. China
2. The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, P. R. China
3. Laboratory of Molecular Evolution and Genome Diversity, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, P. R. China
Abstract:Sequence variation of partial cytochrome b genes between two Coilia species, C. ectenes and C. mystus, was in- vestigated. Of the 402 nucleotides, twenty-seven (6.72%) are polymorphic and all are synonymous substitutions. At the third positions of genetic condon of cytochrome b gene, the two species show an extreme anti-G bias (<4%) and a pronounced bias towards A and C (>68%). There is no amino acid sequence divergence between the partial cytochrome b genes of the two species, indicating a close genetic relationship between them. The k-2p genetic distance of partial cytochrome b segment of the two species is 0.072, suggesting that the species were separated 3.6 Ma ago, in the middle Pliocene. Our result reveals that the cytochrome b gene is an appropriate marker for studies of population genetic structures and phylogeographic pat- terns of the two species.
Keywords:cytochrome b gene  genetic variation  mitochondrial DNA  Coilia ectenes  Coilia mystus
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