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| 运用PCR-DGGE技术对3种原甲藻18S rDNA部分序列的差异性分析 | |
| 引用本文： | 孙静,于志刚,甄毓,张涛,赵丽媛,袁健,米铁柱.运用PCR-DGGE技术对3种原甲藻18S rDNA部分序列的差异性分析[J].中国海洋大学学报(自然科学版),2010,40(2). |
| 作者姓名： | 孙静 于志刚 甄毓 张涛 赵丽媛 袁健 米铁柱 |
| 作者单位： | 1. 中国海洋大学,环境科学与工程学院海洋环境与生态教育部重点实验室,山东,青岛,266003;中国海洋大学,海洋化学化工学院化学理论与工程技术教育部重点实验室,山东,青岛,266003;中国海洋大学,海洋生命学院,山东,青岛,2660 2. 中国海洋大学,海洋化学化工学院化学理论与工程技术教育部重点实验室,山东,青岛,266003 3. 中国海洋大学,环境科学与工程学院海洋环境与生态教育部重点实验室,山东,青岛,266003 |
| 基金项目： | 国家高技术研究发展计划(863计划)，国家自然科学基金 |
| 摘 要： | 采用3对特异性引物,对中国近海常见3种原甲藻的18S rDNA的部分序列进行PCR扩增,运用变性梯度凝胶电泳技术(Denaturing Gradient Gel Electrophoresis,DGGE)对PCR产物进行了差异性分析。结果表明,各对引物具有不同的区分效果,选择包含较多差异碱基的短片段进行扩增,可以对藻种进行更好的区分;PCR-DGGE技术具有较高的灵敏性,不同藻种间即使1个碱基的差异也可以区分开;PCR-DGGE技术可以对3种原甲藻进行区分。 |
| 关 键 词： | 原甲藻 |
Difference Analysis of 18S rDNA Partial Sequences of Three Species of Prorocentrum Using PCR-DGGE |
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| SUN Jing,YU Zhi-Gang,ZHEN Yu,ZHANG Tao,ZHAO Li-Yuan,YUAN Jian,MI Tie-Zhu.Difference Analysis of 18S rDNA Partial Sequences of Three Species of Prorocentrum Using PCR-DGGE[J].Periodical of Ocean University of China,2010,40(2). | |
| Authors: | SUN Jing YU Zhi-Gang ZHEN Yu ZHANG Tao ZHAO Li-Yuan YUAN Jian MI Tie-Zhu |
| Institution: | SUN+Jing1,2,3,YU+Zhi-Gang2,ZHEN+Yu1,ZHANG+Tao1,ZHAO+Li-Yuan1,YUAN+Jian1,MI+Tie-Zhu1(Ocean+University+of+China,1.Key+Laboratory+of+Marine+Environment+,+Ecology,Ministry+of+Education,College+of+Environmental+Science+,+Engineering,2.Key+Laboratory+of+Marine+Chemistry+Theory+,+Technology,College+of+Chemistry+,+Chemical+Engineering,3.College+of+Marine+Life+Sciences,Qingdao+266003,China) |
| Abstract: | Three pairs of primers were applied for the amplification of 18S rDNA partial sequences of three species of Prorocentrum.PCR Products were analyzed by DGGE.Primers developed in this study showed different distinguishing effects.Short PCR amplicons with multiple species-specific differences in base pair composition could be separated by DGGE effectively.PCR-DGGE was a sensitive technique,even only a one-base difference between various species could be discerned.The results demonstrated that PCR-DGGE could be... |
| Keywords: | 18S rDNA PCR-DGGE Prorocentrum 18S rDNA PCR-DGGE |
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