牙鲆外周血细胞显微及亚显微结构的研究

实验研究牙鲆外周血细胞显微及亚显微结构 。光镜观察显示，牙鲆外周血中包括六种类型的血细胞：红细胞、淋巴细胞、血栓细胞、单 核细胞、嗜中性粒细胞和嗜酸性粒细胞，未见嗜碱性粒细胞；淋巴细胞的体积最小，单核细 胞的体积最大；在白细胞中，淋巴细胞数量最多，嗜酸性粒细胞数量最少；此外，还可见到 正在分裂的血栓细胞，说明血栓细胞可在外周血液中经直接分裂产生。电镜研究结果表明， 牙鲆红细胞的细胞质均匀，有少量线粒体和囊泡以及大量的极细颗粒；淋巴细胞核大且中位 、胞质中含较多的游离核糖体，胞体具有短伪足；单核细胞的核偏位且形态不规则，胞质中 有内吞泡和较多的细胞器；颗粒白细胞可根据颗粒的不同形态分为Ⅰ型和Ⅱ型两种类型。同时，对牙鲆血细胞结构与功能的关系进行了较系统的探讨。

The Peripheral Blood Cells of the Bastard Halibut (Paralichthys olivaceus): a Light and Electron Microscope Study

The micro and ultra-structure of peripheral blood cells of the Bastard halibut (paralichthys olivaceus Temminck & Schlegel) were st udied. Under the light microscopy, six main types of blood cells can be recogniz ed: erythrocyte, monocyte, thrombocyte, lymphocyte, neutrophil and eosinophil. B asophil was not found. Among these cells, lymphocyte is the smallest, while mono cyte is the biggest; Lymphocyte is the most abundant leucocytes and eosinophil i s the least. We also found thrombocyte's direct division, which means it can be formed in the peripheral blood. The transmission electron microscopy showed that erythrocyte cytoplasm is even and only has a few mitochondria,vacuoles and many small black granules. Lymphocyte is characterized by the presence of short pesu dopodia, the big center nucleus and many free ribosomes, while monocyte is c haracterized by eccentric nucleus, vacuoles and many organelle. Two distinctive types of granules leucocytes were also described as typeⅠand typeⅡ. We also di scuss the relationships between blood cells structure and its functions.