Estimating periphyton standing crop in streams: a comparison of chlorophyll a sampling and visual assessments

Periphyton standing crop is often measured as chlorophyll a but there is increasing interest in using visual assessments of periphyton cover. Visual methods are rapid and require no sample analysis, but can be regarded as subjective with high inter-operator variability. We investigated variability in periphyton standing crop across operators, rivers and time, as determined visually and from chlorophyll a measurements. We found that visual assessments (comprising percentage cover estimates of up to eight periphyton categories) distinguished sites and occasions as effectively as chlorophyll a. Furthermore, an estimate of chlorophyll a could be derived from the visual assessments. Because our surveys were conducted in only three rivers, general applicability of the derivation of estimated chlorophyll a from the visual assessments warrants further investigation. The current recommendation of 20 views was sufficient for realistic visual assessments of average cover. Overall, our results indicated that inter-operator variability in visual assessments need not be a major concern, given adequate training.