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Genomic cloning and expression of vitellogenin gene from the self-fertilizing fish Rivulus marmoratus (Cyprinodontiformes, Rivulidae)
Authors:Il-Chan Kim  Sung Yeoul Chang  Tim D Williams  Young Ja Kim  Yong-Dal Yoon  Yong-Sung Lee  Eun-Ho Park  Jae-Seong Lee  
Abstract:We cloned the vitellogenin gene from the self-fertilizing fish Rivulus marmoratus, and sequenced 12,326 bp. The number of exons of R. marmoratus and rainbow trout vitellogenin genes were different, and also the splicing junctions are different throughout most of the exons and introns but the amino acid similarity of R. marmoratus vitellogenin gene to other species was rather high. In promoter region of R. marmoratus vitellogenin gene, there were several E2 binding sites and the estrogen response element (ERE). We discuss here the gene structure and expression of R. marmoratus vitellogenin gene.
Keywords:Rivulus marmoratus  Vitellogenin gene  Cloning  Genomic clone  Fish
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