发光细菌法在环渤海污水样品遗传毒性检测中的应用

建立了以经过遗传改造的发光细菌Acinetobacter sp.Rec A为受试物种的环境污染物遗传毒性快速检测方法,该方法最快可在3h内得到毒性评价结果。采用该方法评价了环渤海排污口12份污水样品的遗传毒性。环渤海12个排污口的污水样品均表现出了不同水平的遗传毒性,并呈现出一定的分布特征。其中,高毒水质集中出现在山东半岛污水样品中,中毒水质集中出现在辽东半岛地区,而低毒水质主要集中于京津冀地区。综上,在环渤海的污水样品遗传毒性检测中,这种新型发光细菌法具有快速、灵敏、简便等优点,可为以后近海环境的水质生物毒性检测提供参考依据,具有应用于近海环境水质快速监测与评价的潜力。

USING LUMINESCENT BACTERIA TO DETECT GENOTOXICITY OF SEWAGE IN OUTFALL OF THE BOHAI SEA

We established a fast 3-hour genotoxicity detection method to environmental pollutants using geneticallymodified bioluminescent bacteria Acinetobacter sp. RecA. The method was applied to evaluate the genotoxicity of sewage at 12 outfalls in the Bohai Sea rim. Results show that the genetic toxicity of samples at each sewage outfall was different. The genotoxicity of the northern side of the Shandong Peninsula was high as reflected by the outfalls into Laizhou Bay, followed by the Liaodong Peninsula (outfalls into Liaodong Bay), the intermediate, and the Beijing-Tianjin-Hebei area (outfall into Bohai Bay), the least. The genetic toxicity testing with the luminescent bacteria in water sample in this study has been approved a rapid, sensitive, and simple method; it shall provide a reference in biological toxicity testing for potential applications in monitoring and evaluation of water quality on coastal environment.