| Heat-shock protein 70 expression in shrimp Fenneropenaeus chinensis during thermal and immune-challenged stress |
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| 作者姓名： | 郭振宇 焦传珍 相建海 |
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| 作者单位： | LaboratoryofExperimentalMarineBiology,InstituteofOceanology,CAS,Qingdao266071,China |
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| 基金项目： | Supported by the National Key Fundamental Research Program tudy of the Major Diseases and Resistance Mechanism of Mariculture Organisms?，NSFC，No. 30140017，and the international cooperative program mmunaqua?by the European Commisssion，No. ICA4 |
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| 摘 要： | Using western immunoblotting, we obtained heat-shock protein 70 (HSP70) induction data and distribution in different tissues from shrimp Fenneropenaeus chinensis during thermal and immune-challenged stresses. This is probably the first report of the effects of various stressors on the expression of HSP70 in shrimp.HSP70 was prominently induced in hepatopancreas and gills, but not in muscle, eyestalk and hemolymph, when the shrimp were exposed to heat shock and Vibrio anguillavium-challenged stresses. Cold shock and WSSV treatment had no significant effects on the levels of HSP70 expression in all tissues examined. HSP70 induction was greatest after 2 h exposure to heat shock stress, which was elevated after acute heat shock exposure of 10℃ above ambient temperature.
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| 关 键 词： | HSP70 西部免疫学 热压 免疫挑战性 生物组织 |
Heat-shock protein 70 expression in shrimp Fenneropenaeus chinensis during thermal and immune-challenged stress |
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| GUO Zhenyu,JIAO Chuanzhen XIANG Jianhai.Heat-shock protein 70 expression in shrimp Fenneropenaeus chinensis during thermal and immune-challenged stress[J].Chinese Journal of Oceanology and Limnology,2004,22(4):386-391. |
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| Authors: | GUO Zhenyu JIAO Chuanzhen XIANG Jianhai |
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| Abstract: | |
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| Keywords: | HSP70 shrimp Fenneropenaeus chinensis western immunoblotting thermal stress immune- challenged tissues |
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