Enzymatic determination of glucose in marine environments: Improvement and note of caution |
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Authors: | Roger B. Hanson James Snyder |
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Affiliation: | Skidaway Institute of Oceanography, Savannah, GA 31406 U.S.A. |
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Abstract: | Microheterotrophic glucose uptake kinetics (Vmax and [K + Sn] value) and uptake velocities (v) based on natural glucose concentrations (Sn) suggested that glucose concentrations in the Sapelo Island estuary were overestimated by published enzymatic techniques. Glucose oxidase treatment of water samples before glucose measurement yielded results as much as 50% lower than when this step was omitted. Thin layer chromatography shows that glucose must be separated from other carbohydrates (e.g., fructose) which interfere with the glucose assay. |
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