Enzymatic determination of glucose in marine environments: Improvement and note of caution

Microheterotrophic glucose uptake kinetics (V_{max and K + Sn] value)} and uptake velocities (v) based on natural glucose concentrations (S_n) suggested that glucose concentrations in the Sapelo Island estuary were overestimated by published enzymatic techniques. Glucose oxidase treatment of water samples before glucose measurement yielded results as much as 50% lower than when this step was omitted. Thin layer chromatography shows that glucose must be separated from other carbohydrates (e.g., fructose) which interfere with the glucose assay.