| fSSR分析技术的建立及在大黄鱼(Larimichthys crocea)亲子鉴定中的应用 |
| |
| 引用本文： | 武祥伟,刘贤德,王志勇. fSSR分析技术的建立及在大黄鱼(Larimichthys crocea)亲子鉴定中的应用[J]. 海洋通报, 2011, 30(4): 419-424 |
| |
| 作者姓名： | 武祥伟 刘贤德 王志勇 |
| |
| 作者单位： | 集美大学 水产学院 农业部东海海水健康养殖重点实验室,福建厦门,361021 |
| |
| 基金项目： | 公益性行业（农业）科研专项(200903029-4)；国家自然科学基金项目(30771663)；集美大学优秀青年骨干教师基金（2009C002） |
| |
| 摘 要： | 以大黄鱼(Larimichthys crocea)为材料,对荧光标记微卫星(fSSR)分析体系进行了优化,建立了适合大黄鱼fSSR分析的最佳条件.与常规的银染法相比,该方法成本略高,但其分辨率高,可重复性强,检测效率为银染法的6～10倍.利用优化的fSSR 技术进行了大黄鱼亲于鉴定的研究,结果显示在使用6对引物的情况下...
|
| 关 键 词： | fSSR 大黄鱼 亲子鉴定 Gel-Scan 3000 |
| 收稿时间： | 2010-11-25 |
Condition exploration and optimization for fSSR system and its application in large yellow croaker Larimichthys crocea for parentage assignment |
| |
| WU Xiang-wei,LIU Xian-de and WANG Zhi-yong. Condition exploration and optimization for fSSR system and its application in large yellow croaker Larimichthys crocea for parentage assignment[J]. Marine Science Bulletin, 2011, 30(4): 419-424 |
| |
| Authors: | WU Xiang-wei LIU Xian-de WANG Zhi-yong |
| |
| Affiliation: | Key Laboratory of Healthy Mariculture for East China Sea, Ministry of Agriculture of China, Fisheries College, Jimei University, Xiamen 361021, China;Key Laboratory of Healthy Mariculture for East China Sea, Ministry of Agriculture of China, Fisheries College, Jimei University, Xiamen 361021, China;Key Laboratory of Healthy Mariculture for East China Sea, Ministry of Agriculture of China, Fisheries College, Jimei University, Xiamen 361021, China |
| |
| Abstract: | In this study, the fluorescent labeling microsatellite (fSSR) analysis system was constructed and optimized in larger yellow croaker Larimichthys crocea. Although the cost of the fluorescent method was a little higher than that of the silver-staining method, it was 6~10 times efficient compared with the latter with high resolution and repetition . Furthermore, the parentage assignment rates for two groups of large yellow croaker were both more than 99% using optimized fSSR with six microsatellite loci, which means this technology is very usefull. |
| |
| Keywords: | fSSR Larimichthys crocea paretage assignment Gel-Scan 3000 |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《海洋通报》浏览原始摘要信息 |
|
点击此处可从《海洋通报》下载全文 |
|
