Intercolony variability of skeletal oxygen and carbon isotope signatures of cultured Porites corals: Temperature-controlled experiments

The skeletal oxygen isotope ratio of Porites corals is the most frequently used proxy of past seawater temperature and composition for tropical and subtropical oceans. However, field calibration of the proxy signals is often difficult owing to the dual dependence of skeletal oxygen isotope ratio on temperature and the oxygen isotope composition of water. We conducted tank experiments in which we grew Porites spp. colonies for 142 d in thermostated seawater at five temperature settings between 21°C and 29°C under moderate light intensity of 250 μmol m⁻² s⁻¹ with a 12:12 light:dark photoperiod. A skeletal isotope microprofiling technique applied along the major growth axis of each colony revealed that the oxygen isotope ratios of newly deposited skeleton in most colonies remained almost constant during tank incubation, thus providing an ideal situation for precise calibration of oxygen isotope ratio proxy signals. However, the oxygen isotope ratios displayed an unusually large intercolony variability (∼1‰) at each temperature setting although the mean slope (∼0.15‰ °C⁻¹) obtained for the temperature-skeletal oxygen isotope ratio relationship was close to previous results. The intercolony variations in the oxygen isotope ratios were apparently caused by kinetic isotope effects related to variations in the skeletal growth rate rather than by species-specific variability or genetic differences within species. No correlation was found between skeletal carbon isotope ratios and temperature. The carbon isotope ratios showed significantly inverse correlation with linear growth rates, suggesting a kinetic isotope control at low growth rates. Observed intercolony variability in skeletal carbon isotope ratios (∼5‰) can be partly attributed to growth-rate-related kinetic isotope effects.