| Studies on pathogen of explosive epidemic disease of shrimp Ⅱ.Purification of pathogen |
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| 作者姓名： | Wang Jinxing Liu Changbin Zhang Hongwei Zhao Shuangyi Zhang Yanjun Zhao Jing |
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| 作者单位： | College of Life Sciences，Shandong University，Jinan 250100，China |
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| 基金项目： | This work is funded by a grant from the National Key Task Projects of 8th and 9th Five-year plan program. |
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| 摘 要： | ThisworkisfundedbyagrantfromtheNationalKeyTaskProjectsof8thand9thFive-yearplanprogram.INTRODUCTIONInthepastyears,studiesonthevirusesofpenaeidshrimpshavemadegreatprogressinvari-ouscountries.Rightnow,aboutl4virusesofpenaeidshrimpswerefound,i.e.BP(Baculoviruspenaei,Couch,1974),MBV(Penaeusmonndonbaculovirus,Lightneretal.,1983)orPBV(Penaeusplebejusbaculovirus,Lesteretal.,1987),BMN(Baculovirusmidgutglandnecrosisvirus,Sanaetal.,1981),PHRV(Penaeushybridrod-shapedvirus,Owensetal.,199l),…
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| 关 键 词： | Baculovirus explosive epidemic disease Penaeus chinensis |
| 收稿时间： | 1997/7/10 0:00:00 |
| 修稿时间： | 1997/10/3 0:00:00 |
Studies on pathogen of explosive epidemic disease of shrimp Ⅱ.Purification of pathogen |
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| Wang Jinxing,Liu Changbin,Zhang Hongwei,Zhao Shuangyi,Zhang Yanjun,Zhao Jing.Studies on pathogen of explosive epidemic disease of shrimp Ⅱ.Purification of pathogen[J].Acta Oceanologica Sinica,1998,17(4):531-536. |
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| Authors: | Wang Jinxing Liu Changbin Zhang Hongwei Zhao Shuangyi Zhang Yanjun and Zhao Jing |
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| Institution: | College of Life Sciences, Shandong University, Jinan 250100, China |
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| Abstract: | The pathogen of explosive epidemio disease of farmed Chinese shrimp(Penaeus chinensis)was isolated and purified from the cephalothorax and hepatopancreas of diseased shrimps by means of different speeds of centrifugation and sucrose gradient ultracentrifugation.The results showed that same virus isolated from the diseased shrimps in various regions of China was a kind of baculovirus covered with envelope,which was 95-125 nm in diameter and 360-410 nm in length.The size of the capsid was 80-90 nm×330-350 nm.The nucleic acid of the virus was demonstrated as DNA.The result of artificial infection demonstrated that the virus was the pathogen of explosive epidemic disease of farmed Chinese shrimp. |
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| Keywords: | Baculovirus explosive epidemic disease Penaeus chinensis |
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