| 青藏高原土壤中一株低温原油降解菌的分离鉴定及其原油降解特性 |
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| 引用本文： | 王艺霖,艾雪,李师翁,龙昊知,章高森,张威,刘光琇,陈拓. 青藏高原土壤中一株低温原油降解菌的分离鉴定及其原油降解特性[J]. 冰川冻土, 2015, 37(2): 528-537. DOI: 10.7522/j.issn.1000-0240.2015.0060 |
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| 作者姓名： | 王艺霖 艾雪 李师翁 龙昊知 章高森 张威 刘光琇 陈拓 |
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| 作者单位： | 1. 兰州交通大学 化学与生物工程学院, 甘肃 兰州 730070;2. 甘肃省极端环境微生物资源与工程重点实验室/中国科学院 寒区旱区环境与工程研究所 沙漠与沙漠化重点实验室, 甘肃 兰州 730000;3. 江西农业大学 生物科学与工程学院, 江西 南昌 330045 |
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| 基金项目： | 国家国际科技合作专项项目，甘肃省国际科技合作计划项目，国家自然科学基金 |
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| 摘 要： | 从青藏高原班戈桥地区土壤中分离到一株能利用原油为碳源生长的细菌(BGQ-6). 通过16S rRNA基因序列比对及Biolog GEN Ⅲ鉴定板确定该菌株为Rhodococcus qingshengii. 将生长至对数期的菌株接入MM培养基, 10 ℃、150 rpm条件下培养15 d后, 通过GC法检测到该菌对原油的总降解率为74.14%, 且对直链烷烃、支链烷烃、环烷烃和芳香烃等60种烃类有较高的降解率. 通过特异性基因扩增检测到该菌株基因组中具有4个alkB和1个almA两种烷烃羟化酶基因.
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| 关 键 词： | 原油降解菌 红球菌属 alkB almA 青藏高原 |
| 收稿时间： | 2014-10-19 |
| 修稿时间： | 2015-02-03 |
Isolation,identification and degradation characteristics of a low temperature crude oil degrading bacteria strain from the soil of Tibetan Plateau |
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| WANG Yilin,AI Xue,LI Shiweng,LONG Haozhi,ZHANG Gaosen,ZHANG Wei,LIU Guangxiu,CHEN Tuo. Isolation,identification and degradation characteristics of a low temperature crude oil degrading bacteria strain from the soil of Tibetan Plateau[J]. Journal of Glaciology and Geocryology, 2015, 37(2): 528-537. DOI: 10.7522/j.issn.1000-0240.2015.0060 |
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| Authors: | WANG Yilin AI Xue LI Shiweng LONG Haozhi ZHANG Gaosen ZHANG Wei LIU Guangxiu CHEN Tuo |
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| Affiliation: | 1. School of Chemical and Biological Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China;2. Key Laboratory of Extreme Environmental Microbial Resources and Engineering of Gansu Province/Key Laboratory of Desert and Desertification, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China;3. College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang 330045, China |
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| Abstract: | A bacteria strain (BGQ-6) was isolated from the soil sample in the Bangeqiao area of the Tibetan Plateau. The BGQ-6 can grow well within the medium with crude oil as carbon source. The 16S rRNA gene sequence and Biolog GEN III analysis showed that this strain has a high similarity with Rhodococcus qingshengii, and it is identified as Rhodococcus qingshengii BGQ-6. The strain at the logarithmic growth phase was incubated to the MM medium and cultured at 10 ℃ and 150 rpm for 15 d, and the results of gas chromatograph (GC) detection showed that BGQ-6 strain can degraded by 74.14% of crude oil added in the medium. Further analysis indicates that BGQ-6 is able to degrade 60 kinds of hydrocarbon with a high degradation rate, including alkanes, alkane, cyclones, and aromatic hydrocarbon. Using the method of degenerate PCR, we identified two kinds of oil degrading genes, alkB and almA, which encode hydroxylases, and alkB has 4 members and almA has 1 copy in the genome of BGQ-6. |
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| Keywords: | crude oil degradation bacteria genus Rhodococcus alkB almA Tibetan Plateau |
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