山东近海牙鲆同工酶的生化遗传分析

１９９６年５月，１９９７年１月、１２月和１９９８年４月在山东青荣成近海采集了１３１尾活牙鲆共７１０个生化样品，采用水平淀粉胶和垂直聚丙烯酰胺凝胶电泳的方法，对牙鲆的２５种同工酶进行了分析。结果检测出ＬＤＨ、ＭＤＨ、ＭＥＰ、ＩＤＨＰ、Ｇ３ＰＤＨ、ＡＫ、ＣＫ、ＡＣＰ、ＰＧＭ、ＳＤＨ、ＡＤＨ、ＣＡＴ、ＳＯＤ、ＧＤＨ和ＧＰＩ等１５处同工酶在牙鲆眼睛、肌肉、心脏、肝脏、胸鳍和肾脏等６种组织或器官听表达情况，

BIOCHEMICAL GENETIC ANALYSIS OF ISOZYMES ON THE LEFT-EYED FLOUNDER, PARALICHTHYS OLIVACEUS (T. ET S.) IN THE COASTAL WATERS OF SHANDONG

131 live left-eyed flounders, Paralichthys olivaceus (T. ET S.), were collected from the coastal waters of Qingdao and a fishing farm in Rongcheng City, Shandong Province from May in 1996 to April in 1998. In total, 710 biochemical samples from 6 tissues and organs (eye, muscle, heart, liver, pectoral fin and kidney) were fetched and stored immediately in liquid nitrogen (-196°C). The supernatant of samples to be used for the experiments were extracted by homogenizing and centrifuging. 15 isozymes (LDH, MDH, MEP, IDHP, G3PDH, AK, CK, ACP, PGM, SDH, ADH, CAT, SOD, GDH, GPI) in 3 buffer systems (TC, pH=6.9; EBT, pH=8.9; TG, pH=8.3) were tested by horizontal starch gel and vertical polyacrylamide gel electrophoresis, and their expression in 6 tissues and organs-tissues specify was also tested. The biochemical genetics were analyzed in terms of their locus number, structure, alleles, etc., on the basis of which the basic electrophoretic patterns of these 15 isozymes were gained. The electrophoretic patterns of the rest isozymes were not recorded. Altogether 29 gene loci were recorded, among which 9 gene loci including Ldh-A(alleles, 100, 167), Ldh-C(alleles, 98, 100), Idhp-1(alleles, 78, 100), Acp-1(alleles, 78, 100), Pgm(alleles, 100, 200), Sdh(alleles, 100, 125), Adh(alleles, 70, 100, 150), Cat(alleles, 93, 100, 140) and Gdh(alleles, 100, 130) are polymorph, and the mean proportion of polymorph loci was 31.03%. in the meantime, the expression of Ldh-A and Ldh-B during the biochemical genetic analysis of LDH on Paralichthys olivaceus (T. ET S.), the interrelationships between the activities of isozymes protein and stored condition of samples, and testing of effective genetic markers were also discussed.