| 灰树花糖蛋白中总糖含量的测定 |
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| 引用本文： | 杨晓华,于广利,赵峡,宋乐天,高昊东. 灰树花糖蛋白中总糖含量的测定[J]. 中国海洋大学学报(自然科学版), 2006, 36(6): 929-931 |
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| 作者姓名： | 杨晓华 于广利 赵峡 宋乐天 高昊东 |
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| 作者单位： | 中国海洋大学海洋药物与食品研究所,山东,青岛,266003;中国海洋大学海洋药物与食品研究所,山东,青岛,266003;中国海洋大学海洋药物与食品研究所,山东,青岛,266003;中国海洋大学海洋药物与食品研究所,山东,青岛,266003;中国海洋大学海洋药物与食品研究所,山东,青岛,266003 |
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| 摘 要： | 探讨糖蛋白中总糖含量测定的简捷方法,以2种灰树花糖蛋白(GF6A,GF6B)为原料,分别以硫酸-苯酚法和次亚碘酸钠法对其中的总糖含量进行了比较分析。结果显示,应用前者方法测定多糖含量分别为85.45%和38.46%,采用后者方法测定结果分别为87.03%,36.51%,2种方法测定的结果相近,其中以硫酸-苯酚法因不需对样品进行预水解,方法简便、可靠,更适合于糖蛋白中总糖含量测定。
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| 关 键 词： | 灰树花糖蛋白 硫酸-苯酚 次亚碘酸钠 总糖 |
| 文章编号： | 1672-5174(2006)06-929-03 |
| 收稿时间： | 2005-10-22 |
| 修稿时间： | 2005-12-05 |
Determination of Total Sugar in Glycoprotein of Grifola frondosa |
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| YANG Xiao-Hua,YU Guang-Li,ZHAO Xia,SONG Le-Tian,GAO Hao-Dong. Determination of Total Sugar in Glycoprotein of Grifola frondosa[J]. Periodical of Ocean University of China, 2006, 36(6): 929-931 |
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| Authors: | YANG Xiao-Hua YU Guang-Li ZHAO Xia SONG Le-Tian GAO Hao-Dong |
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| Affiliation: | Institute of Marine Drug and Food, Ocean University of China, Qingdao 266003, China |
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| Abstract: | In this study,the total sugar contents of two glycoproteins(GF6A and GF6B) purified from Grifola frondosa were compared and analyzed by the sulfuric acid-phenol colorimetric method and the NaIO titration method.The experimental results showed that the total sugar contents of GF6A and GF6B were 85.45% and 38.46%,respectively by the sulfuric acid-phenol method,and 87.03% and 36.51%,respectively by the NaIO method.The reliability and stability of the two methods were similar;however,compared to the NaIO method;the sulfuric acid-phenol method was faster and convenient,therefore it is more suitable for the total sugar analysis in glycoprotein. |
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| Keywords: | Grifola frondosa glycoprotein sulfuric acid-phenol NaIO total sugar |
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