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Construction of cDNA Library of Pyrocystis lunula(Pyrophyta)
作者姓名:SUIZhenghong  KlausV.Kowallik
作者单位:Institute of
摘    要:1 Introduction Photosyntheticdinoflagellatesareimportantprima ryproducersandcausesoftoxic‘redtide’ .Theyarefoundinmostaquaticenvironmentsandformamajorpartofthemodernplankton .Dinoflagellatesmaybeimportantindicatorsofenvironmentalstatus (Dale ,1996 ) .…

关 键 词:脱氧核糖核酸  数据库  表达序列  腰鞭毛虫  光合作用
收稿时间:14 April 2004
修稿时间:29 June 2004

Construction of cDNA library of <Emphasis Type="Italic">Pyrocystis lunula</Emphasis> (Pyrophyta)
SUIZhenghong KlausV.Kowallik.Construction of cDNA library of Pyrocystis lunula (Pyrophyta)[J].Journal of Ocean University of China,2004,3(2):141-144.
Authors:Email author" target="_blank">Sui?Zhenghong?Email author  Klaus?V?Kowallik
Institution:1. Department of Bioengineering,Division of Life Science and Technology,Ocean University of China,Qingdao 266003,P.R.China
2. Institute of Botany,Heinrich-Heine-University,Duesseldorf,D-40225,FRG
Abstract:Complementary DNA library of a dinoflagellate Pyrocystis lunula was constructed for the purpose of expression sequence tags analysis. The RNA isolated from this alga was about 20μgg−1 net cells, and the band intensity ratio of 28S/18 S in electrophoresis pattern was nearly 1 to 1. Different cDNA/vector molar ratios were exploited in the ligating reaction to be optimized. The clones produced by cDNA/vector molar ratio of 3.75 to 1 were desirable, most of whose inserts were longer than 300 bp. The recombinants insert length of the unfractionation cDNA library was largely shorter than 500 bp. However, in the fractionation library made from high molecule weight cDNA parts, over seventy percent of the recombinants contained inserts longer than 1 kb, some of which were even longer than 3 kb. Operating concerns were discussed at the end.
Keywords:cDNA library  expression sequence tags  Pyrocystis lunula
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