青弹涂鱼卵巢发育的组织学研究

目前关于青弹涂鱼（Scartelaos virids）的研究多数集中在胚胎和仔鱼发育等个体生物学研究上。本文研究广东省湛江海域中青弹涂鱼在性周期内卵母细胞的发育时相及卵巢结构的周年变化，揭示卵巢发育和卵母细胞的组织学发育规律。结果表明：湛江海域的青弹涂鱼卵母细胞在3月进入大生长期发育阶段，4—6月为繁殖盛期，7—8月为繁殖末期，10月卵巢基本修整完毕，进入Ⅱ恢复期。青弹涂鱼属于一次性产卵类型。在小生长期，卵核的体积较核质的体积增大快，核质比大；大生长期，随着卵黄物质的积累，卵母细胞体积快速增加，核质比下降。最后讨论青弹涂鱼卵膜结构及其生理机能，围核透明层，繁殖习性等问题。

Observation on the ovary development of Scartelaos virids

At present, most studies concerning Scartelaos virids are focused on the development of embryo and young fish. Observation on the ovary sections in different stages of Scartelaos virids from Zhanjing sea area was undertaken. The ovaries began to develop greatly in March, 2007. Most of the ovaries reached Stage Ⅳ and Stage V respectively from April to June, the spawning seasons of Scartelaos virids with highest monthly average of mature coefficient. In October the ovaries developed into Stage Ⅱ and remained in this stage from October to the following February. It is found that they spawn once in each reproductive season. In the course of fast development of oocyte of Scartelaos virids, the nucleus increase in volume more markedly than the cytoplasm during a small growth stage. With the accumulation of yolk substances, the volume of the cytoplasm increases rapidly with a correspondingly sharp drop in the nuclear-plasma ratio, and it reaches to a minimum ratio as the ooeyte becomes full maturity. The structure of the egg choline surface, translucency and reproductive behaviors of Scartelaos virids were also discussed in this paper.