| 3个花鳗鲡地理种群的AFLP分析 |
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| 引用本文： | 朱友芳,林凤钦,王艺磊,谢芳靖.3个花鳗鲡地理种群的AFLP分析[J].海洋科学,2011,35(8):83-88. |
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| 作者姓名： | 朱友芳 林凤钦 王艺磊 谢芳靖 |
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| 作者单位： | 福建省莆田市水产科学研究所;集美大学水产学院 福建省高校水产科学技术与食品安全重点实验室;集美大学水产学院 福建省高校水产科学技术与食品安全重点实验室;集美大学水产学院 福建省高校水产科学技术与食品安全重点实验室 |
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| 基金项目： | 福建省高校水产科学技术与食品安全重点实验室基金资助项目(2008J201) |
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| 摘 要： | 应用AFLP分子标记技术首次对澳大利亚、海南岛和菲律宾3个：仲群的共60尾花鳗鲡（Anguillamarmorata）幼鱼样品进行了遗传多样性分析。4对选择性扩增引物共扩增得到180个位点，平均每对引物扩增出45个位点。澳大利亚、海南岛和菲律宾3个种群的多态位点分别为78．33％、79．44％和84．44％，Nei’s...
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| 关 键 词： | 花鳗鲡似nguilla marmorata) 种群 AFLP 遗传变异 |
| 收稿时间： | 2010/12/8 0:00:00 |
| 修稿时间： | 2011/6/13 0:00:00 |
AFLP analysis of three Anguilla marmorata geographic populations |
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| ZHU You-fang,LIN Feng-qin,WANG Yi-lei,XIE Fang-jing.AFLP analysis of three Anguilla marmorata geographic populations[J].Marine Sciences,2011,35(8):83-88. |
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| Authors: | ZHU You-fang LIN Feng-qin WANG Yi-lei XIE Fang-jing |
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| Institution: | ZHU You-fang1,LIN Feng-qin2,WANG Yi-lei2,XIE Fang-jing2(1.Putian Municipal Institute of Fishery Science,Putian 351100,China,2.The Key Laboratory of Science and Technology for Aquaculture and Food Safety,Fisheries College,Jimei University,Xiamen 361021,China) |
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| Abstract: | This study is the first of its kind to document genetic diversity of giant mottled eel (Anguilla marmorata) among three geographic populations of Australia, Hainan Island, and Philippines. This study was performed on 60 young eels by using AFLP molecular marker technique. A total of 180 AFLP loci, of which 160 loci were polymorphic, were detected by four primer combinations. The percentage of polymorphic loci from Australia, Hainan Island, and Philippines was 78.33%, 79.44%, and 84.44%, respectively; Shannon's diversity index was 0.477 3±0.264 7, 0.484 4±0.270 2, and 0.514 6±0.243 3, respectively. The Gst and AMOVA analysis showed that there were 89.25% and 96.07% genetic variation within the three populations, and 10.75% and 3.93% genetic variation among populations. Our results indicate that genetic differentiation has occurred and has a positive correlation with geographic distant. Meanwhile, gene flows exist among all three populations. These data will be important for the farming management of A. marmorata. |
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| Keywords: | Anguilla marmorata population AFLP genetic variation |
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