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Screening on DHA-producing Antarctic bacteria N- 6 and its cultural conditions
作者姓名:张波涛  缪锦来  回寒星  王卿
作者单位:[1]Life Sciences and Technology College, Ocean University of China, Qingdao 266003, China [2]Key Laboratory of Marine Bio-active Substances, State Ocean Administration, Qingdao 266061, China [3]Shandong Institute and Laboratory of Geological Sciences , Jinan 250013, China
摘    要:1 IntroductionPolyunsaturated fatty acids (PUFAs) have remained as a natural product due to thesynthetic difficulty of reproducing the methylene interrupted double bond sequence by in-dustrial chemistry. Their significance to animals and humans lies in th…

关 键 词:南极  细菌  气候变化  极地
收稿时间:2006-05-01

Screening on DHA-producing Antarctic bacteria N- 6 and its cultural conditions
Zhang Botao,Miao Jin-lai,Hui Hanxing,Wang Qing.Screening on DHA-producing Antarctic bacteria N- 6 and its cultural conditions[J].Chinese Journal of Polar Science,2006,17(1):55-60.
Authors:Zhang Botao  Miao Jin-lai  Hui Hanxing  Wang Qing
Abstract:In Antarctic, the geography and climate differs from those in other places, and the baeteria there have adapted well to the environment there. Two hundred strains of bacteria were isolated from the sea ice in Antarctica. The bacteria were sereened for DHA by means of GC, with fish oil as the standard. Seven strains eontaining DHA or EPA were obtained, among whieh the strain of No. N-6 was outstanding. And the eomponent of DHA was identified by CC-MS. The relative eontent of DHA in N-6 was 8.72%, and total lipids in dry bacteria was 22.54%. The effects of some faetors, ineluding temperature, salinity and pH value, on the growth and DHA-content of strain N-6 were studied. The results show that the DHA-content is relatively high when in low temperature and high pH, and the bacterium is psyehrophilic, alkalophilie.
Keywords:Antarctic  bacteria  GC-MS  unsaturated fatty acids
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